Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01535 and MMP_RS01540 are separated by 9 nucleotides MMP_RS01540 and MMP_RS01545 are separated by 5 nucleotides MMP_RS01545 and MMP_RS01550 are separated by 56 nucleotides MMP_RS01550 and MMP_RS01555 are separated by 23 nucleotides
MMP_RS01535: MMP_RS01535 - DHH family phosphoesterase, at 286,576 to 288,036
_RS01535
MMP_RS01540: MMP_RS01540 - DUF504 domain-containing protein, at 288,046 to 288,276
_RS01540
MMP_RS01545: MMP_RS01545 - CBS domain-containing protein, at 288,282 to 289,076
_RS01545
MMP_RS01550: MMP_RS01550 - DUF1624 domain-containing protein, at 289,133 to 289,852
_RS01550
MMP_RS01555: MMP_RS01555 - hydrogenase formation protein HypD, at 289,876 to 290,958
_RS01555
Position (kb) 288
289
290 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 287.494 kb on - strand at 287.494 kb on - strand at 287.768 kb on - strand at 287.779 kb on - strand at 287.781 kb on - strand at 287.782 kb on + strand at 287.813 kb on - strand at 287.841 kb on + strand at 287.841 kb on + strand at 288.044 kb on - strand at 288.104 kb on + strand, within MMP_RS01540 at 288.111 kb on + strand, within MMP_RS01540 at 288.590 kb on - strand, within MMP_RS01545 at 289.001 kb on - strand at 289.082 kb on + strand at 289.167 kb on - strand at 289.178 kb on + strand at 289.206 kb on + strand, within MMP_RS01550 at 289.279 kb on - strand, within MMP_RS01550 at 289.297 kb on - strand, within MMP_RS01550 at 289.298 kb on + strand, within MMP_RS01550 at 289.340 kb on - strand, within MMP_RS01550 at 289.340 kb on - strand, within MMP_RS01550 at 289.341 kb on + strand, within MMP_RS01550 at 289.390 kb on - strand, within MMP_RS01550 at 289.391 kb on + strand, within MMP_RS01550 at 289.480 kb on - strand, within MMP_RS01550 at 289.611 kb on + strand, within MMP_RS01550 at 289.773 kb on - strand, within MMP_RS01550 at 289.782 kb on - strand at 289.851 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 287,494 - -0.3 287,494 - -2.2 287,768 - -1.1 287,779 - -1.2 287,781 - -1.6 287,782 + -1.6 287,813 - -2.3 287,841 + -1.2 287,841 + -1.5 288,044 - -0.4 288,104 + MMP_RS01540 0.25 -0.1 288,111 + MMP_RS01540 0.28 -0.9 288,590 - MMP_RS01545 0.39 -0.9 289,001 - +0.9 289,082 + -2.7 289,167 - +0.8 289,178 + +0.4 289,206 + MMP_RS01550 0.10 -4.4 289,279 - MMP_RS01550 0.20 +0.5 289,297 - MMP_RS01550 0.23 -0.3 289,298 + MMP_RS01550 0.23 +0.6 289,340 - MMP_RS01550 0.29 -1.3 289,340 - MMP_RS01550 0.29 +1.8 289,341 + MMP_RS01550 0.29 -0.3 289,390 - MMP_RS01550 0.36 -0.3 289,391 + MMP_RS01550 0.36 -1.5 289,480 - MMP_RS01550 0.48 -0.8 289,611 + MMP_RS01550 0.66 -1.5 289,773 - MMP_RS01550 0.89 +0.4 289,782 - -1.0 289,851 + -2.6
Or see this region's nucleotide sequence