Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01515 and MMP_RS01520 are separated by 1 nucleotides MMP_RS01520 and MMP_RS01525 are separated by 188 nucleotides MMP_RS01525 and MMP_RS01530 are separated by 150 nucleotides
MMP_RS01515: MMP_RS01515 - PINc/VapC family ATPase, at 281,507 to 283,399
_RS01515
MMP_RS01520: MMP_RS01520 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 283,401 to 283,877
_RS01520
MMP_RS01525: MMP_RS01525 - nucleoside-diphosphate kinase, at 284,066 to 284,485
_RS01525
MMP_RS01530: MMP_RS01530 - translation initiation factor IF-2, at 284,636 to 286,441
_RS01530
Position (kb)
284
285 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 283.111 kb on + strand, within MMP_RS01515 at 283.317 kb on - strand at 283.929 kb on - strand at 283.933 kb on - strand at 283.933 kb on - strand at 283.934 kb on + strand at 283.960 kb on + strand at 283.995 kb on - strand at 283.995 kb on - strand at 283.995 kb on - strand at 283.996 kb on + strand at 284.000 kb on - strand at 284.006 kb on + strand at 284.062 kb on - strand at 284.226 kb on + strand, within MMP_RS01525 at 284.538 kb on - strand at 284.539 kb on + strand at 284.545 kb on - strand at 284.549 kb on - strand at 284.557 kb on - strand at 284.557 kb on - strand at 284.709 kb on - strand at 284.709 kb on - strand at 284.988 kb on - strand at 285.166 kb on + strand at 285.274 kb on + strand at 285.283 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 283,111 + MMP_RS01515 0.85 -0.8 283,317 - -4.4 283,929 - -1.4 283,933 - -4.2 283,933 - +0.9 283,934 + -0.8 283,960 + -3.6 283,995 - -0.2 283,995 - +0.2 283,995 - -3.4 283,996 + +0.2 284,000 - +0.8 284,006 + +0.5 284,062 - -2.7 284,226 + MMP_RS01525 0.38 -2.5 284,538 - +0.4 284,539 + -1.6 284,545 - -0.2 284,549 - -1.3 284,557 - +2.6 284,557 - -1.3 284,709 - -2.1 284,709 - -0.2 284,988 - -2.5 285,166 + -0.5 285,274 + -1.1 285,283 + +0.9
Or see this region's nucleotide sequence