Strain Fitness in Methanococcus maripaludis S2 around MMP_RS01525

Experiment: Formate McCas no stress control 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS01515 and MMP_RS01520 are separated by 1 nucleotidesMMP_RS01520 and MMP_RS01525 are separated by 188 nucleotidesMMP_RS01525 and MMP_RS01530 are separated by 150 nucleotides MMP_RS01515: MMP_RS01515 - PINc/VapC family ATPase, at 281,507 to 283,399 _RS01515 MMP_RS01520: MMP_RS01520 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 283,401 to 283,877 _RS01520 MMP_RS01525: MMP_RS01525 - nucleoside-diphosphate kinase, at 284,066 to 284,485 _RS01525 MMP_RS01530: MMP_RS01530 - translation initiation factor IF-2, at 284,636 to 286,441 _RS01530 Position (kb) 284 285Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 283.111 kb on + strand, within MMP_RS01515at 283.317 kb on - strandat 283.929 kb on - strandat 283.933 kb on - strandat 283.933 kb on - strandat 283.934 kb on + strandat 283.960 kb on + strandat 283.995 kb on - strandat 283.995 kb on - strandat 283.995 kb on - strandat 283.996 kb on + strandat 284.000 kb on - strandat 284.006 kb on + strandat 284.062 kb on - strandat 284.226 kb on + strand, within MMP_RS01525at 284.538 kb on - strandat 284.539 kb on + strandat 284.545 kb on - strandat 284.549 kb on - strandat 284.557 kb on - strandat 284.557 kb on - strandat 284.709 kb on - strandat 284.709 kb on - strandat 284.988 kb on - strandat 285.166 kb on + strandat 285.274 kb on + strandat 285.283 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas no stress control 2
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283,111 + MMP_RS01515 0.85 -0.8
283,317 - -4.4
283,929 - -1.4
283,933 - -4.2
283,933 - +0.9
283,934 + -0.8
283,960 + -3.6
283,995 - -0.2
283,995 - +0.2
283,995 - -3.4
283,996 + +0.2
284,000 - +0.8
284,006 + +0.5
284,062 - -2.7
284,226 + MMP_RS01525 0.38 -2.5
284,538 - +0.4
284,539 + -1.6
284,545 - -0.2
284,549 - -1.3
284,557 - +2.6
284,557 - -1.3
284,709 - -2.1
284,709 - -0.2
284,988 - -2.5
285,166 + -0.5
285,274 + -1.1
285,283 + +0.9

Or see this region's nucleotide sequence