Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01465 and MMP_RS01470 are separated by 25 nucleotides MMP_RS01470 and MMP_RS01475 are separated by 18 nucleotides MMP_RS01475 and MMP_RS01480 are separated by 211 nucleotides
MMP_RS01465: MMP_RS01465 - hypothetical protein, at 270,743 to 271,441
_RS01465
MMP_RS01470: MMP_RS01470 - ATP-binding cassette domain-containing protein, at 271,467 to 272,258
_RS01470
MMP_RS01475: MMP_RS01475 - phosphosulfolactate synthase, at 272,277 to 273,032
_RS01475
MMP_RS01480: MMP_RS01480 - hydrogenase expression/formation protein HypE, at 273,244 to 274,248
_RS01480
Position (kb) 272
273
274 Strain fitness (log2 ratio) -2
-1
0
1
2 at 271.396 kb on - strand at 271.686 kb on - strand, within MMP_RS01470 at 271.767 kb on - strand, within MMP_RS01470 at 271.839 kb on - strand, within MMP_RS01470 at 271.890 kb on - strand, within MMP_RS01470 at 271.974 kb on + strand, within MMP_RS01470 at 272.187 kb on - strand at 272.382 kb on - strand, within MMP_RS01475 at 272.388 kb on + strand, within MMP_RS01475 at 272.427 kb on - strand, within MMP_RS01475 at 272.428 kb on + strand, within MMP_RS01475 at 272.428 kb on + strand, within MMP_RS01475 at 272.750 kb on - strand, within MMP_RS01475 at 272.764 kb on - strand, within MMP_RS01475 at 272.794 kb on + strand, within MMP_RS01475 at 272.794 kb on + strand, within MMP_RS01475 at 272.819 kb on + strand, within MMP_RS01475 at 272.819 kb on + strand, within MMP_RS01475 at 272.931 kb on - strand, within MMP_RS01475 at 272.937 kb on - strand, within MMP_RS01475 at 273.052 kb on + strand at 273.102 kb on - strand at 273.103 kb on + strand at 273.105 kb on + strand at 273.119 kb on + strand at 273.121 kb on + strand at 273.122 kb on - strand at 273.210 kb on - strand at 273.225 kb on - strand at 273.293 kb on - strand at 273.294 kb on + strand at 273.423 kb on - strand at 273.684 kb on + strand at 273.965 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 271,396 - +2.4 271,686 - MMP_RS01470 0.28 -2.7 271,767 - MMP_RS01470 0.38 +1.5 271,839 - MMP_RS01470 0.47 -1.3 271,890 - MMP_RS01470 0.53 -2.6 271,974 + MMP_RS01470 0.64 +0.5 272,187 - -0.3 272,382 - MMP_RS01475 0.14 +1.6 272,388 + MMP_RS01475 0.15 -1.9 272,427 - MMP_RS01475 0.20 -1.5 272,428 + MMP_RS01475 0.20 -0.0 272,428 + MMP_RS01475 0.20 +0.6 272,750 - MMP_RS01475 0.63 -0.5 272,764 - MMP_RS01475 0.64 -1.1 272,794 + MMP_RS01475 0.68 -2.5 272,794 + MMP_RS01475 0.68 +0.4 272,819 + MMP_RS01475 0.72 -0.6 272,819 + MMP_RS01475 0.72 -1.8 272,931 - MMP_RS01475 0.87 -2.0 272,937 - MMP_RS01475 0.87 -0.7 273,052 + +0.3 273,102 - -2.1 273,103 + -2.4 273,105 + +1.8 273,119 + +0.1 273,121 + +1.2 273,122 - +1.2 273,210 - +0.3 273,225 - -2.4 273,293 - -1.8 273,294 + -0.3 273,423 - -1.5 273,684 + +0.1 273,965 - -0.3
Or see this region's nucleotide sequence