Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01040 and MMP_RS01045 are separated by 89 nucleotides MMP_RS01045 and MMP_RS01050 are separated by 84 nucleotides MMP_RS01050 and MMP_RS01055 are separated by 334 nucleotides
MMP_RS01040: MMP_RS01040 - phosphomethylpyrimidine synthase, at 195,229 to 196,509
_RS01040
MMP_RS01045: MMP_RS01045 - diphthamide biosynthesis enzyme Dph2, at 196,599 to 197,528
_RS01045
MMP_RS01050: MMP_RS01050 - tRNA-Leu, at 197,613 to 197,697
_RS01050
MMP_RS01055: MMP_RS01055 - hypothetical protein, at 198,032 to 198,409
_RS01055
Position (kb) 196
197
198 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 196.329 kb on - strand at 196.330 kb on + strand at 196.540 kb on - strand at 196.542 kb on - strand at 196.554 kb on - strand at 196.558 kb on + strand at 196.560 kb on - strand at 196.560 kb on - strand at 196.560 kb on - strand at 196.564 kb on - strand at 196.564 kb on - strand at 196.565 kb on + strand at 196.565 kb on + strand at 196.599 kb on + strand at 196.781 kb on + strand, within MMP_RS01045 at 196.817 kb on + strand, within MMP_RS01045 at 196.892 kb on - strand, within MMP_RS01045 at 196.959 kb on + strand, within MMP_RS01045 at 196.998 kb on + strand, within MMP_RS01045 at 196.998 kb on + strand, within MMP_RS01045 at 197.096 kb on - strand, within MMP_RS01045 at 197.209 kb on - strand, within MMP_RS01045 at 197.209 kb on - strand, within MMP_RS01045 at 197.322 kb on - strand, within MMP_RS01045 at 197.323 kb on + strand, within MMP_RS01045 at 197.325 kb on + strand, within MMP_RS01045 at 197.443 kb on - strand at 197.443 kb on - strand at 197.470 kb on + strand at 197.516 kb on - strand at 197.524 kb on + strand at 197.811 kb on - strand at 197.856 kb on - strand at 197.894 kb on - strand at 198.086 kb on - strand, within MMP_RS01055 at 198.086 kb on - strand, within MMP_RS01055 at 198.170 kb on + strand, within MMP_RS01055 at 198.406 kb on + strand at 198.406 kb on + strand at 198.411 kb on - strand at 198.411 kb on - strand at 198.412 kb on + strand at 198.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 196,329 - -0.3 196,330 + -1.0 196,540 - -4.4 196,542 - -2.1 196,554 - -1.2 196,558 + -0.6 196,560 - -0.6 196,560 - -3.4 196,560 - -0.1 196,564 - +0.7 196,564 - +0.1 196,565 + -0.3 196,565 + -2.8 196,599 + +1.0 196,781 + MMP_RS01045 0.20 -1.2 196,817 + MMP_RS01045 0.23 +1.7 196,892 - MMP_RS01045 0.32 -0.4 196,959 + MMP_RS01045 0.39 +1.1 196,998 + MMP_RS01045 0.43 +1.1 196,998 + MMP_RS01045 0.43 +0.1 197,096 - MMP_RS01045 0.53 +1.1 197,209 - MMP_RS01045 0.66 +0.8 197,209 - MMP_RS01045 0.66 -2.1 197,322 - MMP_RS01045 0.78 +1.0 197,323 + MMP_RS01045 0.78 -0.3 197,325 + MMP_RS01045 0.78 -3.1 197,443 - +1.6 197,443 - -1.2 197,470 + -0.9 197,516 - -1.4 197,524 + +0.5 197,811 - +1.2 197,856 - -4.4 197,894 - +1.4 198,086 - MMP_RS01055 0.14 -0.1 198,086 - MMP_RS01055 0.14 +1.5 198,170 + MMP_RS01055 0.37 +0.5 198,406 + +2.0 198,406 + +1.2 198,411 - +0.1 198,411 - -1.2 198,412 + -0.9 198,444 - -0.0
Or see this region's nucleotide sequence