Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00820 and MMP_RS00825 are separated by 27 nucleotides MMP_RS00825 and MMP_RS00830 are separated by 15 nucleotides MMP_RS00830 and MMP_RS00835 are separated by 31 nucleotides
MMP_RS00820: MMP_RS00820 - DUF2117 domain-containing protein, at 153,055 to 154,170
_RS00820
MMP_RS00825: MMP_RS00825 - fumarylacetoacetate hydrolase family protein, at 154,198 to 154,806
_RS00825
MMP_RS00830: MMP_RS00830 - purine phosphoribosyltransferase family protein, at 154,822 to 155,379
_RS00830
MMP_RS00835: MMP_RS00835 - FAD-dependent oxidoreductase, at 155,411 to 156,817
_RS00835
Position (kb) 154
155
156 Strain fitness (log2 ratio) -2
-1
0
1 at 154.681 kb on + strand, within MMP_RS00825 at 154.684 kb on - strand, within MMP_RS00825 at 154.684 kb on - strand, within MMP_RS00825 at 154.711 kb on - strand, within MMP_RS00825 at 154.740 kb on - strand, within MMP_RS00825 at 154.798 kb on - strand at 155.145 kb on + strand, within MMP_RS00830 at 155.309 kb on + strand, within MMP_RS00830 at 155.443 kb on + strand at 155.616 kb on - strand at 155.638 kb on - strand at 155.663 kb on + strand at 155.777 kb on + strand at 156.052 kb on + strand at 156.133 kb on - strand at 156.168 kb on + strand at 156.194 kb on + strand at 156.256 kb on - strand at 156.260 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 154,681 + MMP_RS00825 0.79 +0.3 154,684 - MMP_RS00825 0.80 +1.0 154,684 - MMP_RS00825 0.80 +1.4 154,711 - MMP_RS00825 0.84 -1.9 154,740 - MMP_RS00825 0.89 -0.9 154,798 - -0.2 155,145 + MMP_RS00830 0.58 +0.5 155,309 + MMP_RS00830 0.87 -1.7 155,443 + +0.9 155,616 - -0.0 155,638 - +0.0 155,663 + +0.9 155,777 + +0.5 156,052 + -0.2 156,133 - -2.4 156,168 + -0.7 156,194 + -1.7 156,256 - -2.2 156,260 - -2.4
Or see this region's nucleotide sequence