Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00735 and MMP_RS00740 are separated by 306 nucleotides MMP_RS00740 and MMP_RS00745 are separated by 77 nucleotides MMP_RS00745 and MMP_RS00750 are separated by 15 nucleotides
MMP_RS00735: MMP_RS00735 - 5 10-methenyltetrahydromethanopterin hydrogenase, at 133,625 to 134,689
_RS00735
MMP_RS00740: MMP_RS00740 - cyclase family protein, at 134,996 to 135,565
_RS00740
MMP_RS00745: MMP_RS00745 - histidinol phosphate phosphatase domain-containing protein, at 135,643 to 136,299
_RS00745
MMP_RS00750: MMP_RS00750 - fumarate hydratase, at 136,315 to 137,160
_RS00750
Position (kb) 134
135
136 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 134.255 kb on - strand, within MMP_RS00735 at 134.255 kb on - strand, within MMP_RS00735 at 134.424 kb on + strand, within MMP_RS00735 at 134.713 kb on - strand at 134.713 kb on - strand at 134.723 kb on - strand at 134.757 kb on + strand at 134.760 kb on - strand at 134.760 kb on - strand at 134.782 kb on + strand at 134.863 kb on - strand at 134.900 kb on + strand at 134.982 kb on + strand at 135.287 kb on - strand, within MMP_RS00740 at 135.338 kb on + strand, within MMP_RS00740 at 135.421 kb on - strand, within MMP_RS00740 at 135.422 kb on + strand, within MMP_RS00740 at 135.565 kb on - strand at 135.600 kb on + strand at 135.606 kb on + strand at 135.613 kb on - strand at 135.613 kb on - strand at 135.619 kb on + strand at 135.675 kb on - strand at 135.800 kb on - strand at 136.395 kb on + strand at 136.454 kb on - strand, within MMP_RS00750
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 134,255 - MMP_RS00735 0.59 +0.9 134,255 - MMP_RS00735 0.59 -0.2 134,424 + MMP_RS00735 0.75 -1.6 134,713 - -3.3 134,713 - +0.2 134,723 - -1.4 134,757 + -4.3 134,760 - +0.4 134,760 - +0.8 134,782 + -4.4 134,863 - -0.2 134,900 + +1.8 134,982 + -0.5 135,287 - MMP_RS00740 0.51 -1.0 135,338 + MMP_RS00740 0.60 -0.1 135,421 - MMP_RS00740 0.75 +0.1 135,422 + MMP_RS00740 0.75 -0.0 135,565 - +0.8 135,600 + -2.4 135,606 + -2.2 135,613 - -1.2 135,613 - -2.3 135,619 + -0.2 135,675 - -0.3 135,800 - +1.2 136,395 + -0.5 136,454 - MMP_RS00750 0.16 +0.8
Or see this region's nucleotide sequence