Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00695 and MMP_RS00700 are separated by 11 nucleotides MMP_RS00700 and MMP_RS00705 are separated by 12 nucleotides MMP_RS00705 and MMP_RS00710 are separated by 3 nucleotides
MMP_RS00695: MMP_RS00695 - biotin--[acetyl-CoA-carboxylase] ligase, at 126,554 to 127,249
_RS00695
MMP_RS00700: MMP_RS00700 - OB-fold nucleic acid binding domain-containing protein, at 127,261 to 127,647
_RS00700
MMP_RS00705: MMP_RS00705 - hypothetical protein, at 127,660 to 127,977
_RS00705
MMP_RS00710: MMP_RS00710 - hypothetical protein, at 127,981 to 128,889
_RS00710
Position (kb) 127
128 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 126.671 kb on - strand, within MMP_RS00695 at 126.821 kb on - strand, within MMP_RS00695 at 127.200 kb on - strand at 127.262 kb on - strand at 127.276 kb on - strand at 127.283 kb on + strand at 127.327 kb on - strand, within MMP_RS00700 at 127.332 kb on + strand, within MMP_RS00700 at 127.356 kb on - strand, within MMP_RS00700 at 127.496 kb on - strand, within MMP_RS00700 at 127.517 kb on - strand, within MMP_RS00700 at 127.563 kb on + strand, within MMP_RS00700 at 127.831 kb on + strand, within MMP_RS00705 at 127.841 kb on + strand, within MMP_RS00705 at 128.064 kb on + strand at 128.205 kb on - strand, within MMP_RS00710 at 128.546 kb on + strand, within MMP_RS00710 at 128.546 kb on + strand, within MMP_RS00710 at 128.777 kb on + strand, within MMP_RS00710 at 128.800 kb on - strand at 128.800 kb on - strand at 128.829 kb on - strand at 128.858 kb on + strand at 128.858 kb on + strand at 128.859 kb on - strand at 128.859 kb on - strand at 128.967 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 126,671 - MMP_RS00695 0.17 +0.3 126,821 - MMP_RS00695 0.38 -2.1 127,200 - -2.2 127,262 - +0.6 127,276 - -0.8 127,283 + +1.7 127,327 - MMP_RS00700 0.17 +0.3 127,332 + MMP_RS00700 0.18 -0.4 127,356 - MMP_RS00700 0.25 -1.6 127,496 - MMP_RS00700 0.61 +1.1 127,517 - MMP_RS00700 0.66 +0.6 127,563 + MMP_RS00700 0.78 +0.7 127,831 + MMP_RS00705 0.54 -0.2 127,841 + MMP_RS00705 0.57 -4.4 128,064 + -2.8 128,205 - MMP_RS00710 0.25 +0.5 128,546 + MMP_RS00710 0.62 -1.7 128,546 + MMP_RS00710 0.62 +1.9 128,777 + MMP_RS00710 0.88 -0.7 128,800 - +1.1 128,800 - +0.4 128,829 - +0.5 128,858 + -0.6 128,858 + -3.4 128,859 - +1.0 128,859 - -0.0 128,967 - +1.7
Or see this region's nucleotide sequence