Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_01105 and AO356_01110 are separated by 300 nucleotides AO356_01110 and AO356_01115 are separated by 610 nucleotides AO356_01115 and AO356_01120 are separated by 65 nucleotides
AO356_01105: AO356_01105 - putative hydrolase, required for lysine catabolism (from data) , at 251,984 to 253,360
_01105
AO356_01110: AO356_01110 - hypothetical protein, at 253,661 to 254,179
_01110
AO356_01115: AO356_01115 - hypothetical protein, at 254,790 to 255,032
_01115
AO356_01120: AO356_01120 - iron ABC transporter permease, at 255,098 to 256,168
_01120
Position (kb)
253
254
255 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 252.712 kb on + strand, within AO356_01105 at 252.713 kb on - strand, within AO356_01105 at 252.713 kb on - strand, within AO356_01105 at 252.903 kb on - strand, within AO356_01105 at 252.903 kb on - strand, within AO356_01105 at 252.903 kb on - strand, within AO356_01105 at 252.971 kb on + strand, within AO356_01105 at 253.046 kb on + strand, within AO356_01105 at 253.136 kb on + strand, within AO356_01105 at 253.136 kb on + strand, within AO356_01105 at 253.137 kb on - strand, within AO356_01105 at 253.161 kb on - strand, within AO356_01105 at 253.161 kb on - strand, within AO356_01105 at 253.161 kb on - strand, within AO356_01105 at 253.305 kb on - strand at 253.305 kb on - strand at 253.359 kb on - strand at 253.359 kb on - strand at 253.387 kb on + strand at 253.387 kb on + strand at 253.388 kb on - strand at 253.388 kb on - strand at 253.608 kb on - strand at 253.608 kb on - strand at 253.633 kb on + strand at 253.633 kb on + strand at 253.676 kb on + strand at 253.676 kb on + strand at 253.676 kb on + strand at 253.677 kb on - strand at 253.677 kb on - strand at 253.677 kb on - strand at 253.752 kb on - strand, within AO356_01110 at 253.752 kb on - strand, within AO356_01110 at 253.852 kb on - strand, within AO356_01110 at 253.852 kb on - strand, within AO356_01110 at 253.852 kb on - strand, within AO356_01110 at 253.878 kb on + strand, within AO356_01110 at 253.878 kb on + strand, within AO356_01110 at 253.988 kb on + strand, within AO356_01110 at 253.989 kb on - strand, within AO356_01110 at 253.989 kb on - strand, within AO356_01110 at 253.991 kb on + strand, within AO356_01110 at 254.000 kb on - strand, within AO356_01110 at 254.036 kb on + strand, within AO356_01110 at 254.036 kb on + strand, within AO356_01110 at 254.036 kb on + strand, within AO356_01110 at 254.037 kb on - strand, within AO356_01110 at 254.093 kb on + strand, within AO356_01110 at 254.094 kb on - strand, within AO356_01110 at 254.094 kb on - strand, within AO356_01110 at 254.094 kb on - strand, within AO356_01110 at 254.094 kb on - strand, within AO356_01110 at 254.237 kb on - strand at 254.237 kb on - strand at 254.248 kb on - strand at 254.248 kb on - strand at 254.248 kb on - strand at 254.304 kb on - strand at 254.335 kb on + strand at 254.377 kb on + strand at 254.377 kb on + strand at 254.385 kb on + strand at 254.423 kb on + strand at 254.423 kb on + strand at 254.423 kb on + strand at 254.424 kb on - strand at 254.424 kb on - strand at 254.431 kb on + strand at 254.437 kb on + strand at 254.437 kb on + strand at 254.437 kb on + strand at 254.438 kb on - strand at 254.504 kb on - strand at 254.505 kb on + strand at 254.522 kb on + strand at 254.522 kb on + strand at 254.523 kb on - strand at 254.550 kb on - strand at 254.968 kb on + strand, within AO356_01115 at 255.061 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 252,712 + AO356_01105 0.53 +0.2 252,713 - AO356_01105 0.53 -0.6 252,713 - AO356_01105 0.53 -1.6 252,903 - AO356_01105 0.67 -0.4 252,903 - AO356_01105 0.67 +0.3 252,903 - AO356_01105 0.67 +0.1 252,971 + AO356_01105 0.72 +1.2 253,046 + AO356_01105 0.77 -0.8 253,136 + AO356_01105 0.84 +0.0 253,136 + AO356_01105 0.84 -0.5 253,137 - AO356_01105 0.84 +0.2 253,161 - AO356_01105 0.85 -0.4 253,161 - AO356_01105 0.85 -1.3 253,161 - AO356_01105 0.85 +0.6 253,305 - -2.9 253,305 - -0.1 253,359 - -0.8 253,359 - -0.8 253,387 + -0.6 253,387 + -0.4 253,388 - -0.8 253,388 - -0.0 253,608 - -0.6 253,608 - +0.3 253,633 + -0.3 253,633 + -0.8 253,676 + -0.0 253,676 + -1.9 253,676 + +0.5 253,677 - -0.1 253,677 - -0.5 253,677 - -0.7 253,752 - AO356_01110 0.18 +0.2 253,752 - AO356_01110 0.18 -1.0 253,852 - AO356_01110 0.37 -0.3 253,852 - AO356_01110 0.37 +0.4 253,852 - AO356_01110 0.37 +0.3 253,878 + AO356_01110 0.42 +0.3 253,878 + AO356_01110 0.42 -0.1 253,988 + AO356_01110 0.63 -0.9 253,989 - AO356_01110 0.63 -0.2 253,989 - AO356_01110 0.63 -0.7 253,991 + AO356_01110 0.64 -2.1 254,000 - AO356_01110 0.65 -1.9 254,036 + AO356_01110 0.72 -1.0 254,036 + AO356_01110 0.72 -0.2 254,036 + AO356_01110 0.72 -1.2 254,037 - AO356_01110 0.72 +0.8 254,093 + AO356_01110 0.83 -0.4 254,094 - AO356_01110 0.83 -0.1 254,094 - AO356_01110 0.83 -0.9 254,094 - AO356_01110 0.83 -0.5 254,094 - AO356_01110 0.83 +0.2 254,237 - +0.0 254,237 - -0.4 254,248 - -0.3 254,248 - +0.9 254,248 - +0.9 254,304 - -0.3 254,335 + -0.1 254,377 + -0.7 254,377 + -0.7 254,385 + -0.4 254,423 + -0.3 254,423 + -0.7 254,423 + -1.1 254,424 - +1.3 254,424 - +0.8 254,431 + +1.5 254,437 + +0.6 254,437 + -0.1 254,437 + -0.2 254,438 - +1.1 254,504 - +1.1 254,505 + -0.2 254,522 + +0.3 254,522 + -0.8 254,523 - +1.0 254,550 - +0.4 254,968 + AO356_01115 0.73 -0.2 255,061 + -0.3
Or see this region's nucleotide sequence