Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3894 and Pf6N2E2_3895 are separated by 107 nucleotides Pf6N2E2_3895 and Pf6N2E2_3896 are separated by 250 nucleotides
Pf6N2E2_3894 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 4,526,361 to 4,527,587
_3894
Pf6N2E2_3895 - ATP-dependent DNA helicase, RecQ family, at 4,527,695 to 4,529,623
_3895
Pf6N2E2_3896 - possible serine protease homolog, at 4,529,874 to 4,530,938
_3896
Position (kb)
4527
4528
4529
4530 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4526.743 kb on + strand, within Pf6N2E2_3894 at 4526.744 kb on - strand, within Pf6N2E2_3894 at 4526.749 kb on + strand, within Pf6N2E2_3894 at 4526.749 kb on + strand, within Pf6N2E2_3894 at 4526.749 kb on + strand, within Pf6N2E2_3894 at 4526.749 kb on + strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.750 kb on - strand, within Pf6N2E2_3894 at 4526.806 kb on - strand, within Pf6N2E2_3894 at 4526.884 kb on + strand, within Pf6N2E2_3894 at 4526.885 kb on - strand, within Pf6N2E2_3894 at 4526.986 kb on + strand, within Pf6N2E2_3894 at 4526.986 kb on + strand, within Pf6N2E2_3894 at 4526.986 kb on + strand, within Pf6N2E2_3894 at 4526.986 kb on + strand, within Pf6N2E2_3894 at 4526.986 kb on + strand, within Pf6N2E2_3894 at 4526.987 kb on - strand, within Pf6N2E2_3894 at 4526.987 kb on - strand, within Pf6N2E2_3894 at 4526.987 kb on - strand, within Pf6N2E2_3894 at 4526.987 kb on - strand, within Pf6N2E2_3894 at 4526.987 kb on - strand, within Pf6N2E2_3894 at 4526.987 kb on - strand, within Pf6N2E2_3894 at 4526.987 kb on - strand, within Pf6N2E2_3894 at 4527.343 kb on + strand, within Pf6N2E2_3894 at 4527.424 kb on + strand, within Pf6N2E2_3894 at 4527.424 kb on + strand, within Pf6N2E2_3894 at 4527.424 kb on + strand, within Pf6N2E2_3894 at 4527.424 kb on + strand, within Pf6N2E2_3894 at 4527.424 kb on + strand, within Pf6N2E2_3894 at 4527.424 kb on + strand, within Pf6N2E2_3894 at 4527.424 kb on + strand, within Pf6N2E2_3894 at 4527.425 kb on - strand, within Pf6N2E2_3894 at 4527.425 kb on - strand, within Pf6N2E2_3894 at 4527.425 kb on - strand, within Pf6N2E2_3894 at 4527.425 kb on - strand, within Pf6N2E2_3894 at 4527.425 kb on - strand, within Pf6N2E2_3894 at 4527.657 kb on - strand at 4527.739 kb on - strand at 4527.756 kb on + strand at 4527.757 kb on - strand at 4527.757 kb on - strand at 4528.008 kb on + strand, within Pf6N2E2_3895 at 4528.009 kb on - strand, within Pf6N2E2_3895 at 4528.104 kb on + strand, within Pf6N2E2_3895 at 4528.104 kb on + strand, within Pf6N2E2_3895 at 4528.104 kb on + strand, within Pf6N2E2_3895 at 4528.104 kb on + strand, within Pf6N2E2_3895 at 4528.104 kb on + strand, within Pf6N2E2_3895 at 4528.105 kb on - strand, within Pf6N2E2_3895 at 4528.105 kb on - strand, within Pf6N2E2_3895 at 4528.105 kb on - strand, within Pf6N2E2_3895 at 4528.105 kb on - strand, within Pf6N2E2_3895 at 4528.105 kb on - strand, within Pf6N2E2_3895 at 4528.105 kb on - strand, within Pf6N2E2_3895 at 4528.105 kb on - strand, within Pf6N2E2_3895 at 4528.135 kb on + strand, within Pf6N2E2_3895 at 4528.161 kb on + strand, within Pf6N2E2_3895 at 4528.161 kb on + strand, within Pf6N2E2_3895 at 4528.161 kb on + strand, within Pf6N2E2_3895 at 4528.161 kb on + strand, within Pf6N2E2_3895 at 4528.162 kb on - strand, within Pf6N2E2_3895 at 4528.162 kb on - strand, within Pf6N2E2_3895 at 4528.215 kb on + strand, within Pf6N2E2_3895 at 4528.215 kb on + strand, within Pf6N2E2_3895 at 4528.215 kb on + strand, within Pf6N2E2_3895 at 4528.216 kb on - strand, within Pf6N2E2_3895 at 4528.216 kb on - strand, within Pf6N2E2_3895 at 4528.216 kb on - strand, within Pf6N2E2_3895 at 4528.216 kb on - strand, within Pf6N2E2_3895 at 4528.254 kb on + strand, within Pf6N2E2_3895 at 4528.263 kb on + strand, within Pf6N2E2_3895 at 4528.263 kb on + strand, within Pf6N2E2_3895 at 4528.264 kb on - strand, within Pf6N2E2_3895 at 4528.410 kb on + strand, within Pf6N2E2_3895 at 4528.410 kb on + strand, within Pf6N2E2_3895 at 4528.410 kb on + strand, within Pf6N2E2_3895 at 4528.411 kb on - strand, within Pf6N2E2_3895 at 4528.416 kb on + strand, within Pf6N2E2_3895 at 4528.597 kb on - strand, within Pf6N2E2_3895 at 4528.641 kb on - strand, within Pf6N2E2_3895 at 4528.695 kb on + strand, within Pf6N2E2_3895 at 4528.695 kb on + strand, within Pf6N2E2_3895 at 4528.858 kb on - strand, within Pf6N2E2_3895 at 4528.858 kb on - strand, within Pf6N2E2_3895 at 4528.929 kb on + strand, within Pf6N2E2_3895 at 4528.929 kb on + strand, within Pf6N2E2_3895 at 4528.929 kb on + strand, within Pf6N2E2_3895 at 4528.930 kb on - strand, within Pf6N2E2_3895 at 4528.930 kb on - strand, within Pf6N2E2_3895 at 4528.930 kb on - strand, within Pf6N2E2_3895 at 4528.930 kb on - strand, within Pf6N2E2_3895 at 4529.034 kb on + strand, within Pf6N2E2_3895 at 4529.157 kb on + strand, within Pf6N2E2_3895 at 4529.175 kb on + strand, within Pf6N2E2_3895 at 4529.175 kb on + strand, within Pf6N2E2_3895 at 4529.191 kb on + strand, within Pf6N2E2_3895 at 4529.191 kb on + strand, within Pf6N2E2_3895 at 4529.209 kb on + strand, within Pf6N2E2_3895 at 4529.210 kb on - strand, within Pf6N2E2_3895 at 4529.377 kb on + strand, within Pf6N2E2_3895 at 4529.429 kb on - strand, within Pf6N2E2_3895 at 4529.450 kb on + strand at 4529.490 kb on + strand at 4529.490 kb on + strand at 4529.491 kb on - strand at 4529.491 kb on - strand at 4529.719 kb on + strand at 4529.719 kb on + strand at 4529.720 kb on - strand at 4529.720 kb on - strand at 4529.757 kb on - strand at 4529.804 kb on - strand at 4529.818 kb on - strand at 4529.897 kb on - strand at 4529.994 kb on + strand, within Pf6N2E2_3896 at 4529.994 kb on + strand, within Pf6N2E2_3896 at 4530.037 kb on + strand, within Pf6N2E2_3896 at 4530.038 kb on - strand, within Pf6N2E2_3896 at 4530.038 kb on - strand, within Pf6N2E2_3896 at 4530.038 kb on - strand, within Pf6N2E2_3896 at 4530.038 kb on - strand, within Pf6N2E2_3896 at 4530.038 kb on - strand, within Pf6N2E2_3896 at 4530.038 kb on - strand, within Pf6N2E2_3896 at 4530.038 kb on - strand, within Pf6N2E2_3896 at 4530.038 kb on - strand, within Pf6N2E2_3896 at 4530.038 kb on - strand, within Pf6N2E2_3896 at 4530.039 kb on + strand, within Pf6N2E2_3896 at 4530.039 kb on + strand, within Pf6N2E2_3896 at 4530.039 kb on + strand, within Pf6N2E2_3896 at 4530.039 kb on + strand, within Pf6N2E2_3896 at 4530.039 kb on + strand, within Pf6N2E2_3896 at 4530.040 kb on - strand, within Pf6N2E2_3896 at 4530.040 kb on - strand, within Pf6N2E2_3896 at 4530.040 kb on - strand, within Pf6N2E2_3896 at 4530.040 kb on - strand, within Pf6N2E2_3896 at 4530.040 kb on - strand, within Pf6N2E2_3896 at 4530.040 kb on - strand, within Pf6N2E2_3896 at 4530.040 kb on - strand, within Pf6N2E2_3896 at 4530.040 kb on - strand, within Pf6N2E2_3896 at 4530.040 kb on - strand, within Pf6N2E2_3896 at 4530.040 kb on - strand, within Pf6N2E2_3896 at 4530.147 kb on + strand, within Pf6N2E2_3896 at 4530.147 kb on + strand, within Pf6N2E2_3896 at 4530.208 kb on + strand, within Pf6N2E2_3896 at 4530.209 kb on - strand, within Pf6N2E2_3896 at 4530.236 kb on + strand, within Pf6N2E2_3896 at 4530.236 kb on + strand, within Pf6N2E2_3896 at 4530.417 kb on + strand, within Pf6N2E2_3896 at 4530.497 kb on + strand, within Pf6N2E2_3896
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 4,526,743 + Pf6N2E2_3894 0.31 +1.0 4,526,744 - Pf6N2E2_3894 0.31 +0.1 4,526,749 + Pf6N2E2_3894 0.32 +1.2 4,526,749 + Pf6N2E2_3894 0.32 -1.3 4,526,749 + Pf6N2E2_3894 0.32 -0.9 4,526,749 + Pf6N2E2_3894 0.32 +0.2 4,526,750 - Pf6N2E2_3894 0.32 +0.5 4,526,750 - Pf6N2E2_3894 0.32 +0.7 4,526,750 - Pf6N2E2_3894 0.32 -0.3 4,526,750 - Pf6N2E2_3894 0.32 -0.4 4,526,750 - Pf6N2E2_3894 0.32 -0.3 4,526,750 - Pf6N2E2_3894 0.32 -0.6 4,526,806 - Pf6N2E2_3894 0.36 +1.3 4,526,884 + Pf6N2E2_3894 0.43 -0.2 4,526,885 - Pf6N2E2_3894 0.43 +0.3 4,526,986 + Pf6N2E2_3894 0.51 +0.8 4,526,986 + Pf6N2E2_3894 0.51 +0.2 4,526,986 + Pf6N2E2_3894 0.51 +0.7 4,526,986 + Pf6N2E2_3894 0.51 -0.5 4,526,986 + Pf6N2E2_3894 0.51 +0.7 4,526,987 - Pf6N2E2_3894 0.51 +0.3 4,526,987 - Pf6N2E2_3894 0.51 -1.2 4,526,987 - Pf6N2E2_3894 0.51 +0.5 4,526,987 - Pf6N2E2_3894 0.51 +0.1 4,526,987 - Pf6N2E2_3894 0.51 -0.5 4,526,987 - Pf6N2E2_3894 0.51 +0.1 4,526,987 - Pf6N2E2_3894 0.51 -0.7 4,527,343 + Pf6N2E2_3894 0.80 -0.2 4,527,424 + Pf6N2E2_3894 0.87 -1.3 4,527,424 + Pf6N2E2_3894 0.87 +0.2 4,527,424 + Pf6N2E2_3894 0.87 -0.7 4,527,424 + Pf6N2E2_3894 0.87 +0.9 4,527,424 + Pf6N2E2_3894 0.87 -0.3 4,527,424 + Pf6N2E2_3894 0.87 +0.2 4,527,424 + Pf6N2E2_3894 0.87 +1.1 4,527,425 - Pf6N2E2_3894 0.87 -0.8 4,527,425 - Pf6N2E2_3894 0.87 -0.7 4,527,425 - Pf6N2E2_3894 0.87 +0.1 4,527,425 - Pf6N2E2_3894 0.87 +0.1 4,527,425 - Pf6N2E2_3894 0.87 +0.2 4,527,657 - +0.3 4,527,739 - -0.2 4,527,756 + +0.3 4,527,757 - +1.6 4,527,757 - -0.0 4,528,008 + Pf6N2E2_3895 0.16 -1.4 4,528,009 - Pf6N2E2_3895 0.16 -2.1 4,528,104 + Pf6N2E2_3895 0.21 -0.5 4,528,104 + Pf6N2E2_3895 0.21 -0.1 4,528,104 + Pf6N2E2_3895 0.21 +0.1 4,528,104 + Pf6N2E2_3895 0.21 +1.2 4,528,104 + Pf6N2E2_3895 0.21 +0.6 4,528,105 - Pf6N2E2_3895 0.21 -0.2 4,528,105 - Pf6N2E2_3895 0.21 -0.1 4,528,105 - Pf6N2E2_3895 0.21 +2.3 4,528,105 - Pf6N2E2_3895 0.21 +0.8 4,528,105 - Pf6N2E2_3895 0.21 -0.3 4,528,105 - Pf6N2E2_3895 0.21 -0.6 4,528,105 - Pf6N2E2_3895 0.21 +1.4 4,528,135 + Pf6N2E2_3895 0.23 +0.8 4,528,161 + Pf6N2E2_3895 0.24 +0.5 4,528,161 + Pf6N2E2_3895 0.24 +0.1 4,528,161 + Pf6N2E2_3895 0.24 -0.7 4,528,161 + Pf6N2E2_3895 0.24 -0.2 4,528,162 - Pf6N2E2_3895 0.24 -0.1 4,528,162 - Pf6N2E2_3895 0.24 -0.3 4,528,215 + Pf6N2E2_3895 0.27 -0.4 4,528,215 + Pf6N2E2_3895 0.27 -0.6 4,528,215 + Pf6N2E2_3895 0.27 -0.5 4,528,216 - Pf6N2E2_3895 0.27 -0.6 4,528,216 - Pf6N2E2_3895 0.27 -0.9 4,528,216 - Pf6N2E2_3895 0.27 -0.1 4,528,216 - Pf6N2E2_3895 0.27 +0.2 4,528,254 + Pf6N2E2_3895 0.29 -0.2 4,528,263 + Pf6N2E2_3895 0.29 +0.6 4,528,263 + Pf6N2E2_3895 0.29 -0.8 4,528,264 - Pf6N2E2_3895 0.29 +0.3 4,528,410 + Pf6N2E2_3895 0.37 +0.7 4,528,410 + Pf6N2E2_3895 0.37 -1.2 4,528,410 + Pf6N2E2_3895 0.37 +0.7 4,528,411 - Pf6N2E2_3895 0.37 -0.7 4,528,416 + Pf6N2E2_3895 0.37 -0.8 4,528,597 - Pf6N2E2_3895 0.47 -0.4 4,528,641 - Pf6N2E2_3895 0.49 +0.9 4,528,695 + Pf6N2E2_3895 0.52 +0.5 4,528,695 + Pf6N2E2_3895 0.52 +0.5 4,528,858 - Pf6N2E2_3895 0.60 -0.2 4,528,858 - Pf6N2E2_3895 0.60 +0.4 4,528,929 + Pf6N2E2_3895 0.64 -0.7 4,528,929 + Pf6N2E2_3895 0.64 +0.5 4,528,929 + Pf6N2E2_3895 0.64 -0.5 4,528,930 - Pf6N2E2_3895 0.64 -0.7 4,528,930 - Pf6N2E2_3895 0.64 -0.7 4,528,930 - Pf6N2E2_3895 0.64 -0.0 4,528,930 - Pf6N2E2_3895 0.64 -1.6 4,529,034 + Pf6N2E2_3895 0.69 -0.0 4,529,157 + Pf6N2E2_3895 0.76 -0.3 4,529,175 + Pf6N2E2_3895 0.77 +0.7 4,529,175 + Pf6N2E2_3895 0.77 -0.8 4,529,191 + Pf6N2E2_3895 0.78 -0.9 4,529,191 + Pf6N2E2_3895 0.78 -0.2 4,529,209 + Pf6N2E2_3895 0.78 -0.6 4,529,210 - Pf6N2E2_3895 0.79 +0.1 4,529,377 + Pf6N2E2_3895 0.87 -1.4 4,529,429 - Pf6N2E2_3895 0.90 +0.0 4,529,450 + +0.5 4,529,490 + -0.0 4,529,490 + -0.3 4,529,491 - -0.8 4,529,491 - +1.1 4,529,719 + +1.0 4,529,719 + -0.7 4,529,720 - -0.3 4,529,720 - -0.5 4,529,757 - -0.6 4,529,804 - -0.1 4,529,818 - -0.6 4,529,897 - -0.3 4,529,994 + Pf6N2E2_3896 0.11 +0.5 4,529,994 + Pf6N2E2_3896 0.11 +0.2 4,530,037 + Pf6N2E2_3896 0.15 -0.0 4,530,038 - Pf6N2E2_3896 0.15 -0.1 4,530,038 - Pf6N2E2_3896 0.15 -0.4 4,530,038 - Pf6N2E2_3896 0.15 -1.3 4,530,038 - Pf6N2E2_3896 0.15 +0.2 4,530,038 - Pf6N2E2_3896 0.15 +0.0 4,530,038 - Pf6N2E2_3896 0.15 -0.5 4,530,038 - Pf6N2E2_3896 0.15 +1.6 4,530,038 - Pf6N2E2_3896 0.15 +0.8 4,530,038 - Pf6N2E2_3896 0.15 -0.1 4,530,039 + Pf6N2E2_3896 0.15 -1.2 4,530,039 + Pf6N2E2_3896 0.15 +0.2 4,530,039 + Pf6N2E2_3896 0.15 +0.7 4,530,039 + Pf6N2E2_3896 0.15 -0.5 4,530,039 + Pf6N2E2_3896 0.15 +0.6 4,530,040 - Pf6N2E2_3896 0.16 -0.1 4,530,040 - Pf6N2E2_3896 0.16 +0.5 4,530,040 - Pf6N2E2_3896 0.16 -1.6 4,530,040 - Pf6N2E2_3896 0.16 -0.1 4,530,040 - Pf6N2E2_3896 0.16 -0.1 4,530,040 - Pf6N2E2_3896 0.16 +0.1 4,530,040 - Pf6N2E2_3896 0.16 +1.1 4,530,040 - Pf6N2E2_3896 0.16 +0.3 4,530,040 - Pf6N2E2_3896 0.16 +2.6 4,530,040 - Pf6N2E2_3896 0.16 +0.3 4,530,147 + Pf6N2E2_3896 0.26 -0.4 4,530,147 + Pf6N2E2_3896 0.26 -1.2 4,530,208 + Pf6N2E2_3896 0.31 -0.5 4,530,209 - Pf6N2E2_3896 0.31 -0.5 4,530,236 + Pf6N2E2_3896 0.34 -0.5 4,530,236 + Pf6N2E2_3896 0.34 +1.0 4,530,417 + Pf6N2E2_3896 0.51 -0.2 4,530,497 + Pf6N2E2_3896 0.58 -0.2
Or see this region's nucleotide sequence