Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1014 and Pf6N2E2_1015 are separated by 552 nucleotides Pf6N2E2_1015 and Pf6N2E2_1016 are separated by 48 nucleotides
Pf6N2E2_1014 - Nitrate/nitrite transporter, at 1,184,190 to 1,184,381
_1014
Pf6N2E2_1015 - Inositol transport system sugar-binding protein, at 1,184,934 to 1,185,857
_1015
Pf6N2E2_1016 - Protein involved in biosynthesis of mitomycin antibiotics/polyketide fumonisin, at 1,185,906 to 1,187,060
_1016
Position (kb)
1184
1185
1186 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1183.996 kb on + strand at 1183.996 kb on + strand at 1183.997 kb on - strand at 1184.195 kb on + strand at 1184.254 kb on - strand, within Pf6N2E2_1014 at 1184.254 kb on - strand, within Pf6N2E2_1014 at 1184.280 kb on - strand, within Pf6N2E2_1014 at 1184.600 kb on - strand at 1184.600 kb on - strand at 1184.611 kb on + strand at 1184.622 kb on + strand at 1184.700 kb on - strand at 1184.709 kb on + strand at 1184.799 kb on + strand at 1184.799 kb on + strand at 1184.799 kb on + strand at 1184.800 kb on - strand at 1184.801 kb on + strand at 1184.801 kb on + strand at 1184.801 kb on + strand at 1184.801 kb on + strand at 1184.802 kb on - strand at 1184.816 kb on - strand at 1184.845 kb on - strand at 1184.845 kb on - strand at 1184.847 kb on + strand at 1184.847 kb on + strand at 1184.847 kb on + strand at 1184.847 kb on + strand at 1184.847 kb on + strand at 1184.848 kb on - strand at 1184.848 kb on - strand at 1184.848 kb on - strand at 1184.866 kb on - strand at 1184.956 kb on + strand at 1184.957 kb on - strand at 1184.980 kb on + strand at 1185.260 kb on + strand, within Pf6N2E2_1015 at 1185.260 kb on + strand, within Pf6N2E2_1015 at 1185.261 kb on - strand, within Pf6N2E2_1015 at 1185.343 kb on - strand, within Pf6N2E2_1015 at 1185.535 kb on + strand, within Pf6N2E2_1015 at 1185.535 kb on + strand, within Pf6N2E2_1015 at 1185.535 kb on + strand, within Pf6N2E2_1015 at 1185.666 kb on + strand, within Pf6N2E2_1015 at 1185.733 kb on + strand, within Pf6N2E2_1015 at 1185.734 kb on - strand, within Pf6N2E2_1015 at 1185.835 kb on + strand at 1185.901 kb on + strand at 1185.902 kb on - strand at 1185.927 kb on + strand at 1185.958 kb on + strand at 1185.958 kb on + strand at 1186.110 kb on + strand, within Pf6N2E2_1016 at 1186.145 kb on - strand, within Pf6N2E2_1016 at 1186.336 kb on + strand, within Pf6N2E2_1016 at 1186.377 kb on + strand, within Pf6N2E2_1016 at 1186.377 kb on + strand, within Pf6N2E2_1016 at 1186.378 kb on - strand, within Pf6N2E2_1016 at 1186.378 kb on - strand, within Pf6N2E2_1016 at 1186.381 kb on + strand, within Pf6N2E2_1016 at 1186.381 kb on + strand, within Pf6N2E2_1016 at 1186.381 kb on + strand, within Pf6N2E2_1016 at 1186.382 kb on - strand at 1186.382 kb on - strand, within Pf6N2E2_1016 at 1186.382 kb on - strand, within Pf6N2E2_1016 at 1186.466 kb on - strand, within Pf6N2E2_1016 at 1186.534 kb on + strand, within Pf6N2E2_1016 at 1186.651 kb on + strand, within Pf6N2E2_1016 at 1186.651 kb on + strand, within Pf6N2E2_1016 at 1186.652 kb on - strand, within Pf6N2E2_1016 at 1186.652 kb on - strand, within Pf6N2E2_1016 at 1186.654 kb on + strand, within Pf6N2E2_1016 at 1186.655 kb on - strand, within Pf6N2E2_1016 at 1186.655 kb on - strand, within Pf6N2E2_1016
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 1,183,996 + -0.1 1,183,996 + -0.1 1,183,997 - -1.1 1,184,195 + -0.3 1,184,254 - Pf6N2E2_1014 0.33 +1.6 1,184,254 - Pf6N2E2_1014 0.33 +0.7 1,184,280 - Pf6N2E2_1014 0.47 +0.9 1,184,600 - -1.4 1,184,600 - -0.0 1,184,611 + +0.2 1,184,622 + -0.0 1,184,700 - -1.1 1,184,709 + -0.1 1,184,799 + +1.0 1,184,799 + -0.6 1,184,799 + -0.6 1,184,800 - -0.4 1,184,801 + +2.4 1,184,801 + -1.1 1,184,801 + -0.2 1,184,801 + +1.1 1,184,802 - -0.1 1,184,816 - -0.0 1,184,845 - -0.9 1,184,845 - -0.0 1,184,847 + +0.6 1,184,847 + +0.3 1,184,847 + +0.2 1,184,847 + +0.2 1,184,847 + +0.8 1,184,848 - +0.7 1,184,848 - +0.8 1,184,848 - -0.7 1,184,866 - +1.7 1,184,956 + +0.4 1,184,957 - +1.4 1,184,980 + -0.3 1,185,260 + Pf6N2E2_1015 0.35 -3.1 1,185,260 + Pf6N2E2_1015 0.35 -1.4 1,185,261 - Pf6N2E2_1015 0.35 -2.4 1,185,343 - Pf6N2E2_1015 0.44 -0.6 1,185,535 + Pf6N2E2_1015 0.65 -0.0 1,185,535 + Pf6N2E2_1015 0.65 +1.9 1,185,535 + Pf6N2E2_1015 0.65 -0.3 1,185,666 + Pf6N2E2_1015 0.79 -0.3 1,185,733 + Pf6N2E2_1015 0.86 -0.3 1,185,734 - Pf6N2E2_1015 0.87 +1.4 1,185,835 + -2.1 1,185,901 + +0.8 1,185,902 - -0.6 1,185,927 + -0.3 1,185,958 + -0.4 1,185,958 + +0.1 1,186,110 + Pf6N2E2_1016 0.18 -0.8 1,186,145 - Pf6N2E2_1016 0.21 -0.4 1,186,336 + Pf6N2E2_1016 0.37 -0.2 1,186,377 + Pf6N2E2_1016 0.41 +0.4 1,186,377 + Pf6N2E2_1016 0.41 -0.5 1,186,378 - Pf6N2E2_1016 0.41 -0.8 1,186,378 - Pf6N2E2_1016 0.41 +0.1 1,186,381 + Pf6N2E2_1016 0.41 -0.8 1,186,381 + Pf6N2E2_1016 0.41 -0.1 1,186,381 + Pf6N2E2_1016 0.41 -1.0 1,186,382 - -0.1 1,186,382 - Pf6N2E2_1016 0.41 +1.7 1,186,382 - Pf6N2E2_1016 0.41 -0.5 1,186,466 - Pf6N2E2_1016 0.48 +0.4 1,186,534 + Pf6N2E2_1016 0.54 -0.4 1,186,651 + Pf6N2E2_1016 0.65 -0.1 1,186,651 + Pf6N2E2_1016 0.65 +0.5 1,186,652 - Pf6N2E2_1016 0.65 -1.0 1,186,652 - Pf6N2E2_1016 0.65 -0.6 1,186,654 + Pf6N2E2_1016 0.65 -0.1 1,186,655 - Pf6N2E2_1016 0.65 +0.3 1,186,655 - Pf6N2E2_1016 0.65 +0.8
Or see this region's nucleotide sequence