Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_26115 and AO356_26120 overlap by 4 nucleotides AO356_26120 and AO356_26125 are separated by 144 nucleotides AO356_26125 and AO356_26130 are separated by 69 nucleotides AO356_26130 and AO356_26135 are separated by 236 nucleotides
AO356_26115: AO356_26115 - (2Fe-2S)-binding protein, at 5,912,214 to 5,913,587
_26115
AO356_26120: AO356_26120 - cytochrome C2, at 5,913,584 to 5,913,934
_26120
AO356_26125: AO356_26125 - ABC transporter substrate-binding protein, at 5,914,079 to 5,914,861
_26125
AO356_26130: AO356_26130 - hypothetical protein, at 5,914,931 to 5,915,356
_26130
AO356_26135: AO356_26135 - AraC family transcriptional regulator, at 5,915,593 to 5,916,429
_26135
Position (kb)
5914
5915 Strain fitness (log2 ratio)
-1
0
1
2 at 5913.226 kb on - strand, within AO356_26115 at 5913.232 kb on + strand, within AO356_26115 at 5913.233 kb on - strand, within AO356_26115 at 5913.296 kb on + strand, within AO356_26115 at 5913.297 kb on - strand, within AO356_26115 at 5913.504 kb on + strand at 5913.507 kb on + strand at 5913.590 kb on - strand at 5913.590 kb on - strand at 5913.590 kb on - strand at 5913.590 kb on - strand at 5914.055 kb on + strand at 5914.055 kb on + strand at 5914.056 kb on - strand at 5914.238 kb on + strand, within AO356_26125 at 5914.238 kb on + strand, within AO356_26125 at 5914.239 kb on - strand, within AO356_26125 at 5914.450 kb on + strand, within AO356_26125 at 5914.508 kb on - strand, within AO356_26125 at 5914.508 kb on - strand, within AO356_26125 at 5914.520 kb on + strand, within AO356_26125 at 5914.520 kb on + strand, within AO356_26125 at 5914.565 kb on + strand, within AO356_26125 at 5914.715 kb on + strand, within AO356_26125 at 5914.715 kb on + strand, within AO356_26125 at 5914.715 kb on + strand, within AO356_26125 at 5914.716 kb on - strand, within AO356_26125 at 5914.743 kb on + strand, within AO356_26125 at 5914.811 kb on + strand at 5914.812 kb on - strand at 5914.812 kb on - strand at 5914.812 kb on - strand at 5914.832 kb on + strand at 5914.832 kb on + strand at 5914.833 kb on - strand at 5914.847 kb on - strand at 5914.851 kb on - strand at 5914.851 kb on - strand at 5914.947 kb on + strand at 5915.213 kb on - strand, within AO356_26130 at 5915.453 kb on - strand at 5915.470 kb on + strand at 5915.471 kb on - strand at 5915.474 kb on + strand at 5915.475 kb on - strand at 5915.475 kb on - strand at 5915.475 kb on - strand at 5915.677 kb on + strand, within AO356_26135 at 5915.677 kb on + strand, within AO356_26135 at 5915.678 kb on - strand, within AO356_26135 at 5915.678 kb on - strand, within AO356_26135 at 5915.678 kb on - strand, within AO356_26135 at 5915.854 kb on + strand, within AO356_26135 at 5915.854 kb on + strand, within AO356_26135 at 5915.855 kb on - strand, within AO356_26135
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 5,913,226 - AO356_26115 0.74 +0.2 5,913,232 + AO356_26115 0.74 +0.2 5,913,233 - AO356_26115 0.74 +0.2 5,913,296 + AO356_26115 0.79 +0.8 5,913,297 - AO356_26115 0.79 +0.6 5,913,504 + -0.4 5,913,507 + +0.3 5,913,590 - +1.2 5,913,590 - -0.5 5,913,590 - -0.1 5,913,590 - -0.8 5,914,055 + -0.8 5,914,055 + -1.1 5,914,056 - -0.4 5,914,238 + AO356_26125 0.20 -0.1 5,914,238 + AO356_26125 0.20 +0.6 5,914,239 - AO356_26125 0.20 +1.2 5,914,450 + AO356_26125 0.47 -0.4 5,914,508 - AO356_26125 0.55 +0.1 5,914,508 - AO356_26125 0.55 -0.7 5,914,520 + AO356_26125 0.56 -0.4 5,914,520 + AO356_26125 0.56 -0.2 5,914,565 + AO356_26125 0.62 +0.5 5,914,715 + AO356_26125 0.81 +0.1 5,914,715 + AO356_26125 0.81 +0.3 5,914,715 + AO356_26125 0.81 +0.5 5,914,716 - AO356_26125 0.81 -0.1 5,914,743 + AO356_26125 0.85 +1.0 5,914,811 + +0.5 5,914,812 - -0.3 5,914,812 - +0.8 5,914,812 - +1.1 5,914,832 + +0.2 5,914,832 + +0.3 5,914,833 - -0.1 5,914,847 - +0.9 5,914,851 - +0.3 5,914,851 - -1.5 5,914,947 + +0.1 5,915,213 - AO356_26130 0.66 -0.2 5,915,453 - +0.0 5,915,470 + +2.2 5,915,471 - -0.8 5,915,474 + +0.3 5,915,475 - +0.4 5,915,475 - +1.1 5,915,475 - -0.9 5,915,677 + AO356_26135 0.10 +1.3 5,915,677 + AO356_26135 0.10 +2.0 5,915,678 - AO356_26135 0.10 +1.0 5,915,678 - AO356_26135 0.10 +1.8 5,915,678 - AO356_26135 0.10 +1.9 5,915,854 + AO356_26135 0.31 +2.5 5,915,854 + AO356_26135 0.31 +2.6 5,915,855 - AO356_26135 0.31 +2.7
Or see this region's nucleotide sequence