Experiment: MinMedia 20C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1081 and Shew_1082 are separated by 358 nucleotides Shew_1082 and Shew_1083 overlap by 4 nucleotides Shew_1083 and Shew_1084 are separated by 120 nucleotides
Shew_1081: Shew_1081 - threonine synthase (RefSeq), at 1,245,127 to 1,246,410
_1081
Shew_1082: Shew_1082 - hypothetical protein (RefSeq), at 1,246,769 to 1,247,065
_1082
Shew_1083: Shew_1083 - apurinic endonuclease Apn1 (RefSeq), at 1,247,062 to 1,247,913
_1083
Shew_1084: Shew_1084 - PAS/PAC sensor hybrid histidine kinase (RefSeq), at 1,248,034 to 1,250,070
_1084
Position (kb) 1246
1247
1248 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 1246.027 kb on - strand, within Shew_1081 at 1246.332 kb on + strand at 1246.340 kb on - strand at 1246.377 kb on + strand at 1246.377 kb on + strand at 1246.377 kb on + strand at 1246.385 kb on - strand at 1246.950 kb on - strand, within Shew_1082 at 1247.053 kb on - strand at 1247.445 kb on + strand, within Shew_1083 at 1247.717 kb on + strand, within Shew_1083 at 1247.731 kb on - strand, within Shew_1083 at 1247.831 kb on + strand at 1248.046 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MinMedia 20C remove 1,246,027 - Shew_1081 0.70 -7.6 1,246,332 + -6.6 1,246,340 - -7.4 1,246,377 + +0.9 1,246,377 + +0.0 1,246,377 + -0.5 1,246,385 - -0.4 1,246,950 - Shew_1082 0.61 +0.3 1,247,053 - -0.1 1,247,445 + Shew_1083 0.45 +0.4 1,247,717 + Shew_1083 0.77 -0.4 1,247,731 - Shew_1083 0.79 -0.1 1,247,831 + -0.1 1,248,046 - -0.0
Or see this region's nucleotide sequence