Experiment: MinMedia 20C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trmD and rplS are separated by 34 nucleotides rplS and Shew_1067 are separated by 404 nucleotides Shew_1067 and tyrA overlap by 1 nucleotides
Shew_1065: trmD - tRNA (guanine-N(1)-)-methyltransferase (RefSeq), at 1,226,498 to 1,227,244
trmD
Shew_1066: rplS - 50S ribosomal protein L19 (RefSeq), at 1,227,279 to 1,227,629
rplS
Shew_1067: Shew_1067 - phospho-2-dehydro-3-deoxyheptonate aldolase (RefSeq), at 1,228,034 to 1,229,125
_1067
Shew_1068: tyrA - bifunctional chorismate mutase/prephenate dehydrogenase (RefSeq), at 1,229,125 to 1,230,279
tyrA
Position (kb)
1228
1229
1230 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1227.791 kb on + strand at 1227.822 kb on - strand at 1228.023 kb on - strand at 1228.032 kb on - strand at 1228.163 kb on + strand, within Shew_1067 at 1228.261 kb on + strand, within Shew_1067 at 1228.282 kb on + strand, within Shew_1067 at 1228.337 kb on + strand, within Shew_1067 at 1228.779 kb on + strand, within Shew_1067 at 1228.779 kb on + strand, within Shew_1067 at 1228.956 kb on + strand, within Shew_1067 at 1229.665 kb on - strand, within tyrA
Per-strain Table
Position Strand Gene LocusTag Fraction MinMedia 20C remove 1,227,791 + -0.3 1,227,822 - +0.4 1,228,023 - -1.8 1,228,032 - -2.2 1,228,163 + Shew_1067 0.12 -1.3 1,228,261 + Shew_1067 0.21 -1.7 1,228,282 + Shew_1067 0.23 -1.8 1,228,337 + Shew_1067 0.28 -0.9 1,228,779 + Shew_1067 0.68 -1.9 1,228,779 + Shew_1067 0.68 -2.0 1,228,956 + Shew_1067 0.84 -2.2 1,229,665 - tyrA Shew_1068 0.47 -2.9
Or see this region's nucleotide sequence