Experiment: Survival; Treatment=UGA synthetic gw pH5.4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_18970 and IAI47_18975 are separated by 115 nucleotides IAI47_18975 and IAI47_18980 are separated by 43 nucleotides IAI47_18980 and IAI47_18985 overlap by 4 nucleotides IAI47_18985 and IAI47_18990 are separated by 137 nucleotides
IAI47_18970: IAI47_18970 - hypothetical protein, at 27,782 to 28,102
_18970
IAI47_18975: IAI47_18975 - B3/4 domain-containing protein, at 28,218 to 28,913
_18975
IAI47_18980: IAI47_18980 - helix-turn-helix domain-containing protein, at 28,957 to 29,559
_18980
IAI47_18985: IAI47_18985 - MarR family transcriptional regulator, at 29,556 to 30,071
_18985
IAI47_18990: IAI47_18990 - MFS transporter, at 30,209 to 31,363
_18990
Position (kb) 28
29
30 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 28.005 kb on + strand, within IAI47_18970 at 28.005 kb on + strand, within IAI47_18970 at 28.005 kb on + strand, within IAI47_18970 at 28.006 kb on - strand, within IAI47_18970 at 28.008 kb on + strand, within IAI47_18970 at 28.008 kb on + strand, within IAI47_18970 at 28.008 kb on + strand, within IAI47_18970 at 28.009 kb on - strand, within IAI47_18970 at 28.009 kb on - strand, within IAI47_18970 at 28.009 kb on - strand, within IAI47_18970 at 28.009 kb on - strand, within IAI47_18970 at 28.084 kb on + strand at 28.085 kb on - strand at 28.086 kb on + strand at 28.086 kb on + strand at 28.086 kb on + strand at 28.087 kb on - strand at 28.087 kb on - strand at 28.097 kb on - strand at 28.103 kb on + strand at 28.103 kb on + strand at 28.103 kb on + strand at 28.103 kb on + strand at 28.103 kb on + strand at 28.104 kb on - strand at 28.104 kb on - strand at 28.104 kb on - strand at 28.104 kb on - strand at 28.104 kb on - strand at 28.105 kb on + strand at 28.105 kb on + strand at 28.105 kb on + strand at 28.105 kb on + strand at 28.106 kb on - strand at 28.106 kb on - strand at 28.106 kb on - strand at 28.118 kb on + strand at 28.118 kb on + strand at 28.118 kb on + strand at 28.118 kb on + strand at 28.118 kb on + strand at 28.118 kb on + strand at 28.118 kb on + strand at 28.119 kb on - strand at 28.119 kb on - strand at 28.119 kb on - strand at 28.119 kb on - strand at 28.119 kb on - strand at 28.187 kb on + strand at 28.278 kb on + strand at 28.279 kb on - strand at 28.279 kb on - strand at 28.329 kb on - strand, within IAI47_18975 at 28.349 kb on + strand, within IAI47_18975 at 28.350 kb on - strand, within IAI47_18975 at 28.423 kb on - strand, within IAI47_18975 at 28.545 kb on - strand, within IAI47_18975 at 28.546 kb on + strand, within IAI47_18975 at 28.546 kb on + strand, within IAI47_18975 at 28.546 kb on + strand, within IAI47_18975 at 28.546 kb on + strand, within IAI47_18975 at 28.546 kb on + strand, within IAI47_18975 at 28.546 kb on + strand, within IAI47_18975 at 28.546 kb on + strand, within IAI47_18975 at 28.547 kb on - strand, within IAI47_18975 at 28.547 kb on - strand, within IAI47_18975 at 28.547 kb on - strand, within IAI47_18975 at 28.547 kb on - strand, within IAI47_18975 at 28.583 kb on - strand, within IAI47_18975 at 28.603 kb on + strand, within IAI47_18975 at 28.603 kb on + strand, within IAI47_18975 at 28.603 kb on + strand, within IAI47_18975 at 28.603 kb on + strand, within IAI47_18975 at 28.698 kb on - strand, within IAI47_18975 at 28.822 kb on - strand, within IAI47_18975 at 28.830 kb on - strand, within IAI47_18975 at 28.830 kb on - strand, within IAI47_18975 at 28.836 kb on + strand, within IAI47_18975 at 28.836 kb on + strand, within IAI47_18975 at 28.908 kb on + strand at 28.908 kb on + strand at 28.908 kb on + strand at 28.908 kb on + strand at 28.908 kb on + strand at 28.909 kb on - strand at 28.909 kb on - strand at 28.909 kb on - strand at 28.909 kb on - strand at 28.933 kb on + strand at 28.939 kb on - strand at 28.946 kb on - strand at 28.948 kb on + strand at 28.977 kb on - strand at 29.010 kb on + strand at 29.010 kb on + strand at 29.046 kb on + strand, within IAI47_18980 at 29.046 kb on + strand, within IAI47_18980 at 29.047 kb on - strand, within IAI47_18980 at 29.098 kb on - strand, within IAI47_18980 at 29.313 kb on + strand, within IAI47_18980 at 29.325 kb on - strand, within IAI47_18980 at 29.325 kb on - strand, within IAI47_18980 at 29.484 kb on + strand, within IAI47_18980 at 29.484 kb on + strand, within IAI47_18980 at 29.484 kb on + strand, within IAI47_18980 at 29.484 kb on + strand, within IAI47_18980 at 29.485 kb on - strand, within IAI47_18980 at 29.485 kb on - strand, within IAI47_18980 at 29.497 kb on + strand, within IAI47_18980 at 29.497 kb on + strand, within IAI47_18980 at 29.497 kb on + strand, within IAI47_18980 at 29.498 kb on - strand, within IAI47_18980 at 29.498 kb on - strand, within IAI47_18980 at 29.498 kb on - strand, within IAI47_18980 at 29.498 kb on - strand, within IAI47_18980 at 29.523 kb on + strand at 29.579 kb on + strand at 29.580 kb on - strand at 29.580 kb on - strand at 29.580 kb on - strand at 29.580 kb on - strand at 29.750 kb on + strand, within IAI47_18985 at 29.750 kb on + strand, within IAI47_18985 at 29.751 kb on - strand, within IAI47_18985 at 29.751 kb on - strand, within IAI47_18985 at 29.751 kb on - strand, within IAI47_18985 at 29.751 kb on - strand, within IAI47_18985 at 29.751 kb on - strand, within IAI47_18985 at 29.830 kb on - strand, within IAI47_18985 at 29.845 kb on + strand, within IAI47_18985 at 29.901 kb on + strand, within IAI47_18985 at 29.901 kb on + strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 29.902 kb on - strand, within IAI47_18985 at 30.059 kb on + strand at 30.071 kb on + strand at 30.071 kb on + strand at 30.071 kb on + strand at 30.071 kb on + strand at 30.071 kb on + strand at 30.071 kb on + strand at 30.072 kb on - strand at 30.072 kb on - strand at 30.082 kb on + strand at 30.082 kb on + strand at 30.095 kb on + strand at 30.095 kb on + strand at 30.095 kb on + strand at 30.095 kb on + strand at 30.095 kb on + strand at 30.096 kb on - strand at 30.096 kb on - strand at 30.122 kb on + strand at 30.122 kb on + strand at 30.122 kb on + strand at 30.123 kb on - strand at 30.123 kb on - strand at 30.123 kb on - strand at 30.123 kb on - strand at 30.264 kb on + strand at 30.286 kb on + strand at 30.287 kb on - strand at 30.287 kb on - strand at 30.338 kb on - strand, within IAI47_18990 at 30.418 kb on + strand, within IAI47_18990
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH5.4; TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 28,005 + IAI47_18970 0.69 +0.3 28,005 + IAI47_18970 0.69 +1.6 28,005 + IAI47_18970 0.69 -1.0 28,006 - IAI47_18970 0.70 +0.0 28,008 + IAI47_18970 0.70 -1.7 28,008 + IAI47_18970 0.70 -0.5 28,008 + IAI47_18970 0.70 +1.3 28,009 - IAI47_18970 0.71 -1.6 28,009 - IAI47_18970 0.71 +2.1 28,009 - IAI47_18970 0.71 +1.6 28,009 - IAI47_18970 0.71 +1.1 28,084 + +0.6 28,085 - +0.1 28,086 + -0.7 28,086 + -1.1 28,086 + +0.4 28,087 - +0.2 28,087 - +1.0 28,097 - -1.5 28,103 + +3.5 28,103 + -0.9 28,103 + -1.5 28,103 + +0.2 28,103 + -0.3 28,104 - -2.1 28,104 - -0.3 28,104 - +1.3 28,104 - -0.1 28,104 - +0.2 28,105 + +1.5 28,105 + -3.3 28,105 + -1.7 28,105 + -0.7 28,106 - +0.4 28,106 - +0.0 28,106 - -0.1 28,118 + -0.2 28,118 + -1.9 28,118 + +2.5 28,118 + +1.5 28,118 + +1.5 28,118 + +0.2 28,118 + -1.5 28,119 - +2.6 28,119 - -1.0 28,119 - +1.2 28,119 - +1.2 28,119 - -2.6 28,187 + -1.3 28,278 + +0.3 28,279 - +1.2 28,279 - -0.7 28,329 - IAI47_18975 0.16 +2.5 28,349 + IAI47_18975 0.19 +1.3 28,350 - IAI47_18975 0.19 -2.0 28,423 - IAI47_18975 0.29 -0.4 28,545 - IAI47_18975 0.47 -0.0 28,546 + IAI47_18975 0.47 +0.1 28,546 + IAI47_18975 0.47 +0.2 28,546 + IAI47_18975 0.47 +1.1 28,546 + IAI47_18975 0.47 -0.3 28,546 + IAI47_18975 0.47 -1.1 28,546 + IAI47_18975 0.47 +0.3 28,546 + IAI47_18975 0.47 +0.6 28,547 - IAI47_18975 0.47 +1.3 28,547 - IAI47_18975 0.47 -0.7 28,547 - IAI47_18975 0.47 -0.9 28,547 - IAI47_18975 0.47 -2.1 28,583 - IAI47_18975 0.52 -0.3 28,603 + IAI47_18975 0.55 -0.6 28,603 + IAI47_18975 0.55 -0.9 28,603 + IAI47_18975 0.55 -1.5 28,603 + IAI47_18975 0.55 +1.8 28,698 - IAI47_18975 0.69 +0.1 28,822 - IAI47_18975 0.87 +1.6 28,830 - IAI47_18975 0.88 +0.2 28,830 - IAI47_18975 0.88 -0.3 28,836 + IAI47_18975 0.89 -0.5 28,836 + IAI47_18975 0.89 -0.3 28,908 + -0.8 28,908 + +0.8 28,908 + +1.0 28,908 + -0.4 28,908 + -1.0 28,909 - +0.1 28,909 - +0.1 28,909 - -3.2 28,909 - +0.3 28,933 + -1.3 28,939 - -0.3 28,946 - +1.9 28,948 + -3.3 28,977 - +1.1 29,010 + +1.1 29,010 + -0.3 29,046 + IAI47_18980 0.15 -1.7 29,046 + IAI47_18980 0.15 -2.3 29,047 - IAI47_18980 0.15 -1.7 29,098 - IAI47_18980 0.23 +0.6 29,313 + IAI47_18980 0.59 +1.0 29,325 - IAI47_18980 0.61 +1.1 29,325 - IAI47_18980 0.61 -1.3 29,484 + IAI47_18980 0.87 +0.7 29,484 + IAI47_18980 0.87 -1.5 29,484 + IAI47_18980 0.87 -0.9 29,484 + IAI47_18980 0.87 -0.1 29,485 - IAI47_18980 0.88 -0.7 29,485 - IAI47_18980 0.88 +0.6 29,497 + IAI47_18980 0.90 -0.4 29,497 + IAI47_18980 0.90 -1.5 29,497 + IAI47_18980 0.90 -2.3 29,498 - IAI47_18980 0.90 +0.5 29,498 - IAI47_18980 0.90 -0.8 29,498 - IAI47_18980 0.90 -3.1 29,498 - IAI47_18980 0.90 -2.3 29,523 + +0.4 29,579 + -0.0 29,580 - +0.9 29,580 - -1.0 29,580 - +1.5 29,580 - -0.5 29,750 + IAI47_18985 0.38 +1.5 29,750 + IAI47_18985 0.38 -1.3 29,751 - IAI47_18985 0.38 -1.0 29,751 - IAI47_18985 0.38 -1.5 29,751 - IAI47_18985 0.38 -3.3 29,751 - IAI47_18985 0.38 +1.3 29,751 - IAI47_18985 0.38 +0.6 29,830 - IAI47_18985 0.53 -1.1 29,845 + IAI47_18985 0.56 +0.5 29,901 + IAI47_18985 0.67 -0.7 29,901 + IAI47_18985 0.67 -0.3 29,902 - IAI47_18985 0.67 +2.1 29,902 - IAI47_18985 0.67 -2.4 29,902 - IAI47_18985 0.67 -0.5 29,902 - IAI47_18985 0.67 +1.5 29,902 - IAI47_18985 0.67 -0.3 29,902 - IAI47_18985 0.67 +0.3 30,059 + +1.0 30,071 + -2.0 30,071 + -0.4 30,071 + -2.0 30,071 + -0.3 30,071 + -0.1 30,071 + -0.2 30,072 - +0.6 30,072 - -1.0 30,082 + +0.3 30,082 + +1.3 30,095 + +1.6 30,095 + -0.3 30,095 + -0.1 30,095 + -0.2 30,095 + -0.4 30,096 - -1.7 30,096 - -2.4 30,122 + -0.2 30,122 + +0.1 30,122 + -2.3 30,123 - -0.4 30,123 - +0.8 30,123 - +1.0 30,123 - +1.3 30,264 + -2.0 30,286 + +0.4 30,287 - -1.6 30,287 - +1.3 30,338 - IAI47_18990 0.11 -0.1 30,418 + IAI47_18990 0.18 -0.3
Or see this region's nucleotide sequence