Experiment: Recovery broth with 5-Fluorouracil 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17070 and BBR_RS17075 are separated by 26 nucleotides BBR_RS17075 and BBR_RS17080 overlap by 4 nucleotides BBR_RS17080 and BBR_RS17085 are separated by 10 nucleotides
BBR_RS17070: BBR_RS17070 - hypothetical protein, at 1,603,798 to 1,606,149
_RS17070
BBR_RS17075: BBR_RS17075 - dihydrodipicolinate synthase family protein, at 1,606,176 to 1,607,069
_RS17075
BBR_RS17080: BBR_RS17080 - hypothetical protein, at 1,607,066 to 1,607,887
_RS17080
BBR_RS17085: BBR_RS17085 - KR domain-containing protein, at 1,607,898 to 1,608,689
_RS17085
Position (kb)
1606
1607
1608 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1605.193 kb on + strand, within BBR_RS17070 at 1605.216 kb on + strand, within BBR_RS17070 at 1605.374 kb on - strand, within BBR_RS17070 at 1605.384 kb on + strand, within BBR_RS17070 at 1605.435 kb on - strand, within BBR_RS17070 at 1605.435 kb on - strand, within BBR_RS17070 at 1605.435 kb on - strand, within BBR_RS17070 at 1605.439 kb on + strand, within BBR_RS17070 at 1605.467 kb on - strand, within BBR_RS17070 at 1605.471 kb on + strand, within BBR_RS17070 at 1605.479 kb on - strand, within BBR_RS17070 at 1605.498 kb on - strand, within BBR_RS17070 at 1605.498 kb on - strand, within BBR_RS17070 at 1605.542 kb on + strand, within BBR_RS17070 at 1605.551 kb on - strand, within BBR_RS17070 at 1605.567 kb on + strand, within BBR_RS17070 at 1605.575 kb on + strand, within BBR_RS17070 at 1605.580 kb on + strand, within BBR_RS17070 at 1605.588 kb on - strand, within BBR_RS17070 at 1605.594 kb on + strand, within BBR_RS17070 at 1605.610 kb on + strand, within BBR_RS17070 at 1605.624 kb on + strand, within BBR_RS17070 at 1605.700 kb on + strand, within BBR_RS17070 at 1605.828 kb on + strand, within BBR_RS17070 at 1606.011 kb on - strand at 1606.014 kb on - strand at 1606.064 kb on + strand at 1606.067 kb on - strand at 1606.072 kb on - strand at 1606.084 kb on - strand at 1606.084 kb on - strand at 1606.084 kb on - strand at 1606.101 kb on - strand at 1606.101 kb on - strand at 1606.107 kb on + strand at 1606.159 kb on - strand at 1606.196 kb on - strand at 1606.196 kb on - strand at 1606.196 kb on - strand at 1606.223 kb on - strand at 1606.223 kb on - strand at 1606.263 kb on - strand at 1606.279 kb on + strand, within BBR_RS17075 at 1606.309 kb on + strand, within BBR_RS17075 at 1606.387 kb on - strand, within BBR_RS17075 at 1606.392 kb on - strand, within BBR_RS17075 at 1606.397 kb on - strand, within BBR_RS17075 at 1606.434 kb on - strand, within BBR_RS17075 at 1606.468 kb on + strand, within BBR_RS17075 at 1606.468 kb on + strand, within BBR_RS17075 at 1606.468 kb on - strand, within BBR_RS17075 at 1606.476 kb on - strand, within BBR_RS17075 at 1606.476 kb on - strand, within BBR_RS17075 at 1606.535 kb on + strand, within BBR_RS17075 at 1606.564 kb on + strand, within BBR_RS17075 at 1606.616 kb on + strand, within BBR_RS17075 at 1606.629 kb on + strand, within BBR_RS17075 at 1606.681 kb on + strand, within BBR_RS17075 at 1606.689 kb on - strand, within BBR_RS17075 at 1606.691 kb on + strand, within BBR_RS17075 at 1606.691 kb on + strand, within BBR_RS17075 at 1606.701 kb on + strand, within BBR_RS17075 at 1606.721 kb on + strand, within BBR_RS17075 at 1606.750 kb on - strand, within BBR_RS17075 at 1606.768 kb on - strand, within BBR_RS17075 at 1606.787 kb on + strand, within BBR_RS17075 at 1606.806 kb on + strand, within BBR_RS17075 at 1606.828 kb on - strand, within BBR_RS17075 at 1606.932 kb on + strand, within BBR_RS17075 at 1606.944 kb on + strand, within BBR_RS17075 at 1606.997 kb on + strand at 1607.005 kb on - strand at 1607.009 kb on - strand at 1607.049 kb on + strand at 1607.152 kb on + strand, within BBR_RS17080 at 1607.152 kb on + strand, within BBR_RS17080 at 1607.168 kb on + strand, within BBR_RS17080 at 1607.242 kb on + strand, within BBR_RS17080 at 1607.257 kb on + strand, within BBR_RS17080 at 1607.257 kb on + strand, within BBR_RS17080 at 1607.257 kb on + strand, within BBR_RS17080 at 1607.258 kb on + strand, within BBR_RS17080 at 1607.265 kb on - strand, within BBR_RS17080 at 1607.311 kb on - strand, within BBR_RS17080 at 1607.323 kb on + strand, within BBR_RS17080 at 1607.323 kb on + strand, within BBR_RS17080 at 1607.323 kb on + strand, within BBR_RS17080 at 1607.353 kb on + strand, within BBR_RS17080 at 1607.353 kb on + strand, within BBR_RS17080 at 1607.376 kb on - strand, within BBR_RS17080 at 1607.420 kb on + strand, within BBR_RS17080 at 1607.529 kb on - strand, within BBR_RS17080 at 1607.537 kb on - strand, within BBR_RS17080 at 1607.566 kb on + strand, within BBR_RS17080 at 1607.620 kb on + strand, within BBR_RS17080 at 1607.643 kb on + strand, within BBR_RS17080 at 1607.708 kb on + strand, within BBR_RS17080 at 1607.753 kb on + strand, within BBR_RS17080 at 1607.770 kb on + strand, within BBR_RS17080 at 1607.788 kb on - strand, within BBR_RS17080 at 1607.817 kb on - strand at 1607.827 kb on - strand at 1607.906 kb on - strand at 1607.957 kb on - strand at 1607.959 kb on - strand at 1607.959 kb on - strand at 1607.959 kb on - strand at 1607.959 kb on - strand at 1607.963 kb on + strand at 1607.968 kb on + strand at 1607.971 kb on - strand at 1608.019 kb on + strand, within BBR_RS17085 at 1608.027 kb on - strand, within BBR_RS17085 at 1608.027 kb on - strand, within BBR_RS17085 at 1608.027 kb on - strand, within BBR_RS17085 at 1608.027 kb on - strand, within BBR_RS17085
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with 5-Fluorouracil 0.2 mM remove 1,605,193 + BBR_RS17070 0.59 +0.7 1,605,216 + BBR_RS17070 0.60 +0.0 1,605,374 - BBR_RS17070 0.67 +0.2 1,605,384 + BBR_RS17070 0.67 -1.6 1,605,435 - BBR_RS17070 0.70 -1.7 1,605,435 - BBR_RS17070 0.70 +0.5 1,605,435 - BBR_RS17070 0.70 +0.0 1,605,439 + BBR_RS17070 0.70 -0.1 1,605,467 - BBR_RS17070 0.71 -1.1 1,605,471 + BBR_RS17070 0.71 -1.7 1,605,479 - BBR_RS17070 0.71 -0.1 1,605,498 - BBR_RS17070 0.72 +0.2 1,605,498 - BBR_RS17070 0.72 +0.5 1,605,542 + BBR_RS17070 0.74 -0.2 1,605,551 - BBR_RS17070 0.75 -0.2 1,605,567 + BBR_RS17070 0.75 +0.3 1,605,575 + BBR_RS17070 0.76 +0.7 1,605,580 + BBR_RS17070 0.76 -1.0 1,605,588 - BBR_RS17070 0.76 -1.3 1,605,594 + BBR_RS17070 0.76 -1.4 1,605,610 + BBR_RS17070 0.77 -0.7 1,605,624 + BBR_RS17070 0.78 -2.6 1,605,700 + BBR_RS17070 0.81 -0.1 1,605,828 + BBR_RS17070 0.86 -2.0 1,606,011 - -0.1 1,606,014 - -0.7 1,606,064 + -0.5 1,606,067 - -1.3 1,606,072 - +1.3 1,606,084 - -0.6 1,606,084 - -0.5 1,606,084 - -1.3 1,606,101 - +0.1 1,606,101 - -0.4 1,606,107 + -1.3 1,606,159 - -2.5 1,606,196 - +0.1 1,606,196 - -1.9 1,606,196 - -0.3 1,606,223 - +0.2 1,606,223 - +0.1 1,606,263 - +0.5 1,606,279 + BBR_RS17075 0.12 -0.4 1,606,309 + BBR_RS17075 0.15 +1.3 1,606,387 - BBR_RS17075 0.24 -1.3 1,606,392 - BBR_RS17075 0.24 -0.7 1,606,397 - BBR_RS17075 0.25 -0.9 1,606,434 - BBR_RS17075 0.29 +0.0 1,606,468 + BBR_RS17075 0.33 +0.8 1,606,468 + BBR_RS17075 0.33 -0.9 1,606,468 - BBR_RS17075 0.33 -0.2 1,606,476 - BBR_RS17075 0.34 +1.7 1,606,476 - BBR_RS17075 0.34 +0.7 1,606,535 + BBR_RS17075 0.40 -0.5 1,606,564 + BBR_RS17075 0.43 -1.9 1,606,616 + BBR_RS17075 0.49 -0.1 1,606,629 + BBR_RS17075 0.51 -0.7 1,606,681 + BBR_RS17075 0.56 -0.5 1,606,689 - BBR_RS17075 0.57 -0.1 1,606,691 + BBR_RS17075 0.58 +0.2 1,606,691 + BBR_RS17075 0.58 -0.2 1,606,701 + BBR_RS17075 0.59 -0.7 1,606,721 + BBR_RS17075 0.61 +0.6 1,606,750 - BBR_RS17075 0.64 +0.3 1,606,768 - BBR_RS17075 0.66 +0.7 1,606,787 + BBR_RS17075 0.68 +1.2 1,606,806 + BBR_RS17075 0.70 +1.3 1,606,828 - BBR_RS17075 0.73 +0.4 1,606,932 + BBR_RS17075 0.85 +0.2 1,606,944 + BBR_RS17075 0.86 +0.6 1,606,997 + +1.2 1,607,005 - -0.0 1,607,009 - -0.2 1,607,049 + -0.1 1,607,152 + BBR_RS17080 0.10 +1.3 1,607,152 + BBR_RS17080 0.10 +0.1 1,607,168 + BBR_RS17080 0.12 -0.2 1,607,242 + BBR_RS17080 0.21 -0.1 1,607,257 + BBR_RS17080 0.23 -0.6 1,607,257 + BBR_RS17080 0.23 +0.1 1,607,257 + BBR_RS17080 0.23 +1.0 1,607,258 + BBR_RS17080 0.23 -1.4 1,607,265 - BBR_RS17080 0.24 +1.7 1,607,311 - BBR_RS17080 0.30 -0.7 1,607,323 + BBR_RS17080 0.31 -0.1 1,607,323 + BBR_RS17080 0.31 -1.4 1,607,323 + BBR_RS17080 0.31 +1.6 1,607,353 + BBR_RS17080 0.35 -0.3 1,607,353 + BBR_RS17080 0.35 +0.4 1,607,376 - BBR_RS17080 0.38 +0.0 1,607,420 + BBR_RS17080 0.43 +0.2 1,607,529 - BBR_RS17080 0.56 +1.3 1,607,537 - BBR_RS17080 0.57 +0.1 1,607,566 + BBR_RS17080 0.61 +1.3 1,607,620 + BBR_RS17080 0.67 -1.2 1,607,643 + BBR_RS17080 0.70 -1.1 1,607,708 + BBR_RS17080 0.78 -0.1 1,607,753 + BBR_RS17080 0.84 +0.3 1,607,770 + BBR_RS17080 0.86 -1.1 1,607,788 - BBR_RS17080 0.88 -1.3 1,607,817 - +0.0 1,607,827 - -0.6 1,607,906 - -0.4 1,607,957 - -0.6 1,607,959 - -1.4 1,607,959 - -1.5 1,607,959 - -0.5 1,607,959 - -1.0 1,607,963 + +1.8 1,607,968 + -0.4 1,607,971 - +0.6 1,608,019 + BBR_RS17085 0.15 -1.8 1,608,027 - BBR_RS17085 0.16 -0.6 1,608,027 - BBR_RS17085 0.16 +0.8 1,608,027 - BBR_RS17085 0.16 -0.8 1,608,027 - BBR_RS17085 0.16 -1.0
Or see this region's nucleotide sequence