Experiment: Recovery broth with 5-Fluorouracil 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14040 and BBR_RS14045 are separated by 289 nucleotides BBR_RS14045 and BBR_RS14050 are separated by 21 nucleotides BBR_RS14050 and BBR_RS14055 are separated by 6 nucleotides BBR_RS14055 and BBR_RS14060 are separated by 2 nucleotides
BBR_RS14040: BBR_RS14040 - GNAT family N-acetyltransferase, at 926,648 to 927,123
_RS14040
BBR_RS14045: BBR_RS14045 - GntR family transcriptional regulator, at 927,413 to 927,787
_RS14045
BBR_RS14050: BBR_RS14050 - ABC transporter ATP-binding protein, at 927,809 to 928,729
_RS14050
BBR_RS14055: BBR_RS14055 - ABC-2 transporter permease, at 928,736 to 929,449
_RS14055
BBR_RS14060: BBR_RS14060 - ABC transporter, at 929,452 to 930,165
_RS14060
Position (kb) 927
928
929 Strain fitness (log2 ratio) -2
-1
0
1
2 at 926.814 kb on - strand, within BBR_RS14040 at 926.814 kb on - strand, within BBR_RS14040 at 926.852 kb on + strand, within BBR_RS14040 at 926.977 kb on + strand, within BBR_RS14040 at 926.987 kb on + strand, within BBR_RS14040 at 926.987 kb on + strand, within BBR_RS14040 at 926.992 kb on + strand, within BBR_RS14040 at 926.995 kb on - strand, within BBR_RS14040 at 927.019 kb on + strand, within BBR_RS14040 at 927.031 kb on + strand, within BBR_RS14040 at 927.058 kb on + strand, within BBR_RS14040 at 927.058 kb on + strand, within BBR_RS14040 at 927.192 kb on + strand at 927.197 kb on + strand at 927.223 kb on - strand at 927.229 kb on + strand at 927.237 kb on - strand at 927.237 kb on - strand at 927.412 kb on + strand at 927.437 kb on - strand at 927.438 kb on + strand at 927.487 kb on + strand, within BBR_RS14045 at 927.555 kb on + strand, within BBR_RS14045 at 927.555 kb on + strand, within BBR_RS14045 at 927.608 kb on + strand, within BBR_RS14045 at 927.673 kb on + strand, within BBR_RS14045 at 927.673 kb on + strand, within BBR_RS14045 at 927.746 kb on + strand, within BBR_RS14045 at 927.746 kb on + strand, within BBR_RS14045 at 927.747 kb on + strand, within BBR_RS14045 at 927.747 kb on + strand, within BBR_RS14045 at 927.747 kb on + strand, within BBR_RS14045 at 927.781 kb on + strand at 927.858 kb on + strand at 927.868 kb on + strand at 927.899 kb on + strand at 927.909 kb on + strand, within BBR_RS14050 at 927.924 kb on - strand, within BBR_RS14050 at 927.957 kb on + strand, within BBR_RS14050 at 927.967 kb on + strand, within BBR_RS14050 at 927.967 kb on + strand, within BBR_RS14050 at 927.984 kb on + strand, within BBR_RS14050 at 928.033 kb on - strand, within BBR_RS14050 at 928.082 kb on - strand, within BBR_RS14050 at 928.102 kb on - strand, within BBR_RS14050 at 928.109 kb on + strand, within BBR_RS14050 at 928.131 kb on - strand, within BBR_RS14050 at 928.146 kb on - strand, within BBR_RS14050 at 928.164 kb on + strand, within BBR_RS14050 at 928.185 kb on + strand, within BBR_RS14050 at 928.185 kb on + strand, within BBR_RS14050 at 928.185 kb on + strand, within BBR_RS14050 at 928.193 kb on - strand, within BBR_RS14050 at 928.298 kb on + strand, within BBR_RS14050 at 928.328 kb on - strand, within BBR_RS14050 at 928.376 kb on - strand, within BBR_RS14050 at 928.386 kb on + strand, within BBR_RS14050 at 928.386 kb on + strand, within BBR_RS14050 at 928.396 kb on + strand, within BBR_RS14050 at 928.396 kb on + strand, within BBR_RS14050 at 928.404 kb on - strand, within BBR_RS14050 at 928.448 kb on - strand, within BBR_RS14050 at 928.468 kb on + strand, within BBR_RS14050 at 928.468 kb on + strand, within BBR_RS14050 at 928.476 kb on - strand, within BBR_RS14050 at 928.513 kb on + strand, within BBR_RS14050 at 928.566 kb on + strand, within BBR_RS14050 at 928.569 kb on + strand, within BBR_RS14050 at 928.605 kb on + strand, within BBR_RS14050 at 928.614 kb on + strand, within BBR_RS14050 at 928.665 kb on + strand at 928.753 kb on - strand at 928.807 kb on - strand at 928.843 kb on + strand, within BBR_RS14055 at 928.850 kb on + strand, within BBR_RS14055 at 928.882 kb on + strand, within BBR_RS14055 at 928.896 kb on + strand, within BBR_RS14055 at 928.903 kb on - strand, within BBR_RS14055 at 928.918 kb on + strand, within BBR_RS14055 at 928.936 kb on - strand, within BBR_RS14055 at 929.006 kb on - strand, within BBR_RS14055 at 929.146 kb on - strand, within BBR_RS14055 at 929.205 kb on - strand, within BBR_RS14055 at 929.269 kb on + strand, within BBR_RS14055 at 929.303 kb on + strand, within BBR_RS14055 at 929.310 kb on + strand, within BBR_RS14055 at 929.315 kb on + strand, within BBR_RS14055 at 929.323 kb on - strand, within BBR_RS14055 at 929.473 kb on + strand at 929.503 kb on + strand at 929.503 kb on + strand at 929.503 kb on + strand at 929.599 kb on + strand, within BBR_RS14060 at 929.599 kb on + strand, within BBR_RS14060 at 929.606 kb on + strand, within BBR_RS14060 at 929.606 kb on + strand, within BBR_RS14060 at 929.611 kb on + strand, within BBR_RS14060 at 929.611 kb on + strand, within BBR_RS14060 at 929.624 kb on - strand, within BBR_RS14060 at 929.633 kb on - strand, within BBR_RS14060 at 929.662 kb on - strand, within BBR_RS14060 at 929.669 kb on + strand, within BBR_RS14060 at 929.697 kb on + strand, within BBR_RS14060 at 929.697 kb on + strand, within BBR_RS14060 at 929.697 kb on + strand, within BBR_RS14060 at 929.697 kb on + strand, within BBR_RS14060 at 929.697 kb on + strand, within BBR_RS14060 at 929.697 kb on + strand, within BBR_RS14060 at 929.698 kb on - strand, within BBR_RS14060 at 929.705 kb on - strand, within BBR_RS14060
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with 5-Fluorouracil 0.2 mM remove 926,814 - BBR_RS14040 0.35 +1.3 926,814 - BBR_RS14040 0.35 -0.4 926,852 + BBR_RS14040 0.43 -0.5 926,977 + BBR_RS14040 0.69 -0.5 926,987 + BBR_RS14040 0.71 -0.6 926,987 + BBR_RS14040 0.71 -0.3 926,992 + BBR_RS14040 0.72 +0.7 926,995 - BBR_RS14040 0.73 -0.3 927,019 + BBR_RS14040 0.78 +1.5 927,031 + BBR_RS14040 0.80 -0.7 927,058 + BBR_RS14040 0.86 +0.7 927,058 + BBR_RS14040 0.86 -0.1 927,192 + +1.4 927,197 + -0.5 927,223 - -0.7 927,229 + -0.3 927,237 - +1.5 927,237 - -1.0 927,412 + +0.5 927,437 - +1.7 927,438 + -1.1 927,487 + BBR_RS14045 0.20 -0.1 927,555 + BBR_RS14045 0.38 -0.1 927,555 + BBR_RS14045 0.38 -0.4 927,608 + BBR_RS14045 0.52 +0.9 927,673 + BBR_RS14045 0.69 -0.1 927,673 + BBR_RS14045 0.69 -1.5 927,746 + BBR_RS14045 0.89 -0.6 927,746 + BBR_RS14045 0.89 -1.3 927,747 + BBR_RS14045 0.89 -0.1 927,747 + BBR_RS14045 0.89 +0.5 927,747 + BBR_RS14045 0.89 -1.6 927,781 + +1.5 927,858 + +0.5 927,868 + +0.6 927,899 + -0.3 927,909 + BBR_RS14050 0.11 -1.0 927,924 - BBR_RS14050 0.12 -1.6 927,957 + BBR_RS14050 0.16 -1.1 927,967 + BBR_RS14050 0.17 +0.1 927,967 + BBR_RS14050 0.17 -0.2 927,984 + BBR_RS14050 0.19 -0.9 928,033 - BBR_RS14050 0.24 -0.8 928,082 - BBR_RS14050 0.30 +0.3 928,102 - BBR_RS14050 0.32 +1.8 928,109 + BBR_RS14050 0.33 -0.7 928,131 - BBR_RS14050 0.35 -0.9 928,146 - BBR_RS14050 0.37 +0.3 928,164 + BBR_RS14050 0.39 -0.6 928,185 + BBR_RS14050 0.41 -0.3 928,185 + BBR_RS14050 0.41 +1.9 928,185 + BBR_RS14050 0.41 -1.4 928,193 - BBR_RS14050 0.42 -1.6 928,298 + BBR_RS14050 0.53 +0.6 928,328 - BBR_RS14050 0.56 -0.2 928,376 - BBR_RS14050 0.62 -0.6 928,386 + BBR_RS14050 0.63 -0.2 928,386 + BBR_RS14050 0.63 -2.0 928,396 + BBR_RS14050 0.64 +0.9 928,396 + BBR_RS14050 0.64 -0.3 928,404 - BBR_RS14050 0.65 -1.4 928,448 - BBR_RS14050 0.69 -0.7 928,468 + BBR_RS14050 0.72 -1.1 928,468 + BBR_RS14050 0.72 -1.1 928,476 - BBR_RS14050 0.72 -0.5 928,513 + BBR_RS14050 0.76 -1.1 928,566 + BBR_RS14050 0.82 -0.9 928,569 + BBR_RS14050 0.83 +1.8 928,605 + BBR_RS14050 0.86 -1.3 928,614 + BBR_RS14050 0.87 -1.0 928,665 + -1.2 928,753 - -0.0 928,807 - +1.0 928,843 + BBR_RS14055 0.15 -0.3 928,850 + BBR_RS14055 0.16 -1.4 928,882 + BBR_RS14055 0.20 -0.7 928,896 + BBR_RS14055 0.22 +0.9 928,903 - BBR_RS14055 0.23 +1.0 928,918 + BBR_RS14055 0.25 -0.1 928,936 - BBR_RS14055 0.28 +0.5 929,006 - BBR_RS14055 0.38 -0.6 929,146 - BBR_RS14055 0.57 +0.7 929,205 - BBR_RS14055 0.66 +0.4 929,269 + BBR_RS14055 0.75 -0.7 929,303 + BBR_RS14055 0.79 +1.0 929,310 + BBR_RS14055 0.80 +0.1 929,315 + BBR_RS14055 0.81 -1.2 929,323 - BBR_RS14055 0.82 -0.1 929,473 + -0.7 929,503 + -1.0 929,503 + +1.0 929,503 + +1.2 929,599 + BBR_RS14060 0.21 -0.1 929,599 + BBR_RS14060 0.21 -0.3 929,606 + BBR_RS14060 0.22 -1.2 929,606 + BBR_RS14060 0.22 +1.6 929,611 + BBR_RS14060 0.22 +0.8 929,611 + BBR_RS14060 0.22 -0.1 929,624 - BBR_RS14060 0.24 +0.5 929,633 - BBR_RS14060 0.25 -0.1 929,662 - BBR_RS14060 0.29 +1.5 929,669 + BBR_RS14060 0.30 -2.4 929,697 + BBR_RS14060 0.34 +0.2 929,697 + BBR_RS14060 0.34 +2.5 929,697 + BBR_RS14060 0.34 -0.4 929,697 + BBR_RS14060 0.34 +1.4 929,697 + BBR_RS14060 0.34 -0.2 929,697 + BBR_RS14060 0.34 -0.3 929,698 - BBR_RS14060 0.34 +0.2 929,705 - BBR_RS14060 0.35 +0.5
Or see this region's nucleotide sequence