Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_23385 and AO356_23390 are separated by 186 nucleotides AO356_23390 and AO356_23395 overlap by 4 nucleotides AO356_23395 and AO356_23400 overlap by 7 nucleotides
AO356_23385: AO356_23385 - short-chain dehydrogenase, at 5,244,570 to 5,245,313
_23385
AO356_23390: AO356_23390 - phosphonate ABC transporter permease, at 5,245,500 to 5,246,267
_23390
AO356_23395: AO356_23395 - ABC transporter permease, at 5,246,264 to 5,247,091
_23395
AO356_23400: AO356_23400 - phosphonate ABC transporter, at 5,247,085 to 5,247,885
_23400
Position (kb) 5245
5246
5247 Strain fitness (log2 ratio) -1
0
1
2 at 5244.521 kb on + strand at 5244.521 kb on + strand at 5244.521 kb on + strand at 5244.521 kb on + strand at 5244.522 kb on - strand at 5244.539 kb on + strand at 5244.539 kb on + strand at 5244.539 kb on + strand at 5244.540 kb on - strand at 5244.540 kb on - strand at 5244.540 kb on - strand at 5244.540 kb on - strand at 5244.540 kb on - strand at 5244.540 kb on - strand at 5244.551 kb on + strand at 5244.551 kb on + strand at 5244.614 kb on - strand at 5244.614 kb on - strand at 5244.871 kb on + strand, within AO356_23385 at 5244.871 kb on + strand, within AO356_23385 at 5244.871 kb on + strand, within AO356_23385 at 5244.871 kb on + strand, within AO356_23385 at 5244.872 kb on - strand, within AO356_23385 at 5244.872 kb on - strand, within AO356_23385 at 5244.872 kb on - strand, within AO356_23385 at 5245.060 kb on - strand, within AO356_23385 at 5245.281 kb on + strand at 5245.281 kb on + strand at 5245.282 kb on - strand at 5245.287 kb on - strand at 5245.287 kb on - strand at 5245.328 kb on - strand at 5245.328 kb on - strand at 5245.375 kb on - strand at 5245.375 kb on - strand at 5245.378 kb on - strand at 5245.415 kb on + strand at 5245.442 kb on + strand at 5245.443 kb on - strand at 5245.542 kb on - strand at 5245.621 kb on + strand, within AO356_23390 at 5245.621 kb on + strand, within AO356_23390 at 5245.621 kb on + strand, within AO356_23390 at 5245.622 kb on - strand, within AO356_23390 at 5245.622 kb on - strand, within AO356_23390 at 5245.622 kb on - strand, within AO356_23390 at 5245.622 kb on - strand, within AO356_23390 at 5245.622 kb on - strand, within AO356_23390 at 5245.622 kb on - strand, within AO356_23390 at 5245.622 kb on - strand, within AO356_23390 at 5245.696 kb on + strand, within AO356_23390 at 5245.705 kb on + strand, within AO356_23390 at 5245.706 kb on - strand, within AO356_23390 at 5245.759 kb on - strand, within AO356_23390 at 5245.808 kb on + strand, within AO356_23390 at 5246.140 kb on + strand, within AO356_23390 at 5246.141 kb on - strand, within AO356_23390 at 5246.503 kb on - strand, within AO356_23395 at 5246.586 kb on + strand, within AO356_23395 at 5246.610 kb on + strand, within AO356_23395 at 5246.610 kb on + strand, within AO356_23395 at 5246.610 kb on + strand, within AO356_23395 at 5246.610 kb on + strand, within AO356_23395 at 5246.614 kb on + strand, within AO356_23395 at 5246.630 kb on + strand, within AO356_23395 at 5247.158 kb on + strand at 5247.159 kb on - strand at 5247.159 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 5,244,521 + -0.3 5,244,521 + -0.4 5,244,521 + +1.0 5,244,521 + +0.3 5,244,522 - +0.3 5,244,539 + -0.2 5,244,539 + +0.9 5,244,539 + +0.2 5,244,540 - -0.4 5,244,540 - -0.2 5,244,540 - +0.3 5,244,540 - +0.4 5,244,540 - +0.5 5,244,540 - -0.5 5,244,551 + +0.4 5,244,551 + -0.5 5,244,614 - +0.3 5,244,614 - -0.1 5,244,871 + AO356_23385 0.40 +0.4 5,244,871 + AO356_23385 0.40 +1.2 5,244,871 + AO356_23385 0.40 +0.2 5,244,871 + AO356_23385 0.40 +0.4 5,244,872 - AO356_23385 0.41 +2.2 5,244,872 - AO356_23385 0.41 -0.3 5,244,872 - AO356_23385 0.41 -0.3 5,245,060 - AO356_23385 0.66 -1.4 5,245,281 + -0.1 5,245,281 + +0.4 5,245,282 - -0.2 5,245,287 - -1.6 5,245,287 - +0.6 5,245,328 - +0.9 5,245,328 - +0.9 5,245,375 - -0.6 5,245,375 - +0.3 5,245,378 - -0.9 5,245,415 + -0.3 5,245,442 + +0.6 5,245,443 - +0.2 5,245,542 - +0.3 5,245,621 + AO356_23390 0.16 -0.4 5,245,621 + AO356_23390 0.16 -0.4 5,245,621 + AO356_23390 0.16 +0.9 5,245,622 - AO356_23390 0.16 +0.0 5,245,622 - AO356_23390 0.16 -0.7 5,245,622 - AO356_23390 0.16 +0.9 5,245,622 - AO356_23390 0.16 -1.1 5,245,622 - AO356_23390 0.16 +0.6 5,245,622 - AO356_23390 0.16 -0.7 5,245,622 - AO356_23390 0.16 -0.7 5,245,696 + AO356_23390 0.26 +0.9 5,245,705 + AO356_23390 0.27 +0.4 5,245,706 - AO356_23390 0.27 +0.5 5,245,759 - AO356_23390 0.34 +0.4 5,245,808 + AO356_23390 0.40 -0.6 5,246,140 + AO356_23390 0.83 +0.1 5,246,141 - AO356_23390 0.83 -0.2 5,246,503 - AO356_23395 0.29 -0.9 5,246,586 + AO356_23395 0.39 -1.2 5,246,610 + AO356_23395 0.42 +1.5 5,246,610 + AO356_23395 0.42 -0.4 5,246,610 + AO356_23395 0.42 -0.4 5,246,610 + AO356_23395 0.42 +0.5 5,246,614 + AO356_23395 0.42 +0.6 5,246,630 + AO356_23395 0.44 +0.5 5,247,158 + +1.6 5,247,159 - -1.5 5,247,159 - +1.1
Or see this region's nucleotide sequence