Experiment: LB 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dut and slmA are separated by 130 nucleotides slmA and Shew_3485 are separated by 149 nucleotides Shew_3485 and folE are separated by 225 nucleotides folE and pyrE are separated by 81 nucleotides
Shew_3483: dut - deoxyuridine 5'-triphosphate nucleotidohydrolase (RefSeq), at 4,168,723 to 4,169,181
dut
Shew_3484: slmA - nucleoid occlusion protein (RefSeq), at 4,169,312 to 4,169,905
slmA
Shew_3485: Shew_3485 - peptidase S9 prolyl oligopeptidase (RefSeq), at 4,170,055 to 4,172,019
_3485
Shew_3486: folE - GTP cyclohydrolase I (RefSeq), at 4,172,245 to 4,172,889
folE
Shew_3487: pyrE - orotate phosphoribosyltransferase (RefSeq), at 4,172,971 to 4,173,612
pyrE
Position (kb) 4170
4171
4172
4173 Strain fitness (log2 ratio) -2
-1
0
1 at 4169.174 kb on - strand at 4169.256 kb on + strand at 4169.759 kb on - strand, within slmA at 4169.867 kb on - strand at 4170.290 kb on + strand, within Shew_3485 at 4170.378 kb on + strand, within Shew_3485 at 4170.396 kb on - strand, within Shew_3485 at 4170.421 kb on - strand, within Shew_3485 at 4170.603 kb on + strand, within Shew_3485 at 4170.611 kb on - strand, within Shew_3485 at 4170.754 kb on + strand, within Shew_3485 at 4170.773 kb on - strand, within Shew_3485 at 4170.826 kb on + strand, within Shew_3485 at 4170.834 kb on - strand, within Shew_3485 at 4170.867 kb on + strand, within Shew_3485 at 4171.229 kb on + strand, within Shew_3485 at 4171.367 kb on + strand, within Shew_3485 at 4171.367 kb on + strand, within Shew_3485 at 4171.375 kb on - strand, within Shew_3485 at 4172.116 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 30C remove 4,169,174 - -0.0 4,169,256 + -0.2 4,169,759 - slmA Shew_3484 0.75 -1.9 4,169,867 - -0.4 4,170,290 + Shew_3485 0.12 -0.6 4,170,378 + Shew_3485 0.16 +0.6 4,170,396 - Shew_3485 0.17 -0.4 4,170,421 - Shew_3485 0.19 +0.3 4,170,603 + Shew_3485 0.28 +0.2 4,170,611 - Shew_3485 0.28 +0.4 4,170,754 + Shew_3485 0.36 -0.2 4,170,773 - Shew_3485 0.37 +0.1 4,170,826 + Shew_3485 0.39 +0.1 4,170,834 - Shew_3485 0.40 -0.4 4,170,867 + Shew_3485 0.41 +0.2 4,171,229 + Shew_3485 0.60 +0.1 4,171,367 + Shew_3485 0.67 +0.1 4,171,367 + Shew_3485 0.67 +0.4 4,171,375 - Shew_3485 0.67 -0.0 4,172,116 + -0.1
Or see this region's nucleotide sequence