Experiment: LB 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0740 and Shew_0741 are separated by 86 nucleotides Shew_0741 and Shew_0742 are separated by 141 nucleotides Shew_0742 and Shew_0743 are separated by 164 nucleotides
Shew_0740: Shew_0740 - L-serine dehydratase 1 (RefSeq), at 842,057 to 843,424
_0740
Shew_0741: Shew_0741 - GCN5-related N-acetyltransferase (RefSeq), at 843,511 to 843,954
_0741
Shew_0742: Shew_0742 - hypothetical protein (RefSeq), at 844,096 to 844,401
_0742
Shew_0743: Shew_0743 - sodium:dicarboxylate symporter (RefSeq), at 844,566 to 845,867
_0743
Position (kb) 843
844 Strain fitness (log2 ratio) -1
0
1 at 842.557 kb on - strand, within Shew_0740 at 842.864 kb on - strand, within Shew_0740 at 842.984 kb on - strand, within Shew_0740 at 843.375 kb on + strand at 843.716 kb on + strand, within Shew_0741 at 843.846 kb on + strand, within Shew_0741 at 843.874 kb on - strand, within Shew_0741 at 843.998 kb on - strand at 844.056 kb on - strand at 844.432 kb on + strand at 844.447 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 30C remove 842,557 - Shew_0740 0.37 +0.1 842,864 - Shew_0740 0.59 -0.6 842,984 - Shew_0740 0.68 +0.3 843,375 + -0.3 843,716 + Shew_0741 0.46 +0.2 843,846 + Shew_0741 0.75 +0.3 843,874 - Shew_0741 0.82 +0.5 843,998 - -0.1 844,056 - -1.0 844,432 + +0.1 844,447 + -0.1
Or see this region's nucleotide sequence