Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2783 and Dshi_2784 are separated by 53 nucleotides Dshi_2784 and Dshi_2785 are separated by 33 nucleotides Dshi_2785 and Dshi_2786 are separated by 4 nucleotides Dshi_2786 and Dshi_2787 are separated by 36 nucleotides
Dshi_2783: Dshi_2783 - N-acetylmuramyl-L-alanine amidase, negative regulator of AmpC, AmpD (RefSeq), at 2,943,475 to 2,944,113
_2783
Dshi_2784: Dshi_2784 - hypothetical protein (RefSeq), at 2,944,167 to 2,944,769
_2784
Dshi_2785: Dshi_2785 - glutamyl-tRNA(Gln) amidotransferase, A subunit (RefSeq), at 2,944,803 to 2,946,290
_2785
Dshi_2786: Dshi_2786 - glutamyl-tRNA(Gln) amidotransferase, C subunit (RefSeq), at 2,946,295 to 2,946,582
_2786
Dshi_2787: Dshi_2787 - hypothetical protein (RefSeq), at 2,946,619 to 2,947,284
_2787
Position (kb)
2944
2945
2946
2947 Strain fitness (log2 ratio)
-2
-1
0
1 at 2943.881 kb on - strand, within Dshi_2783 at 2944.022 kb on + strand, within Dshi_2783 at 2944.077 kb on + strand at 2944.118 kb on - strand at 2946.637 kb on + strand at 2946.670 kb on - strand at 2946.684 kb on + strand at 2946.696 kb on + strand, within Dshi_2787 at 2946.798 kb on - strand, within Dshi_2787 at 2946.812 kb on + strand, within Dshi_2787 at 2946.812 kb on + strand, within Dshi_2787 at 2946.820 kb on - strand, within Dshi_2787 at 2946.874 kb on + strand, within Dshi_2787 at 2946.948 kb on - strand, within Dshi_2787 at 2946.960 kb on + strand, within Dshi_2787 at 2946.983 kb on + strand, within Dshi_2787 at 2947.010 kb on + strand, within Dshi_2787 at 2947.068 kb on + strand, within Dshi_2787 at 2947.075 kb on + strand, within Dshi_2787 at 2947.083 kb on - strand, within Dshi_2787 at 2947.201 kb on + strand, within Dshi_2787 at 2947.231 kb on - strand at 2947.243 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 2,943,881 - Dshi_2783 0.64 -2.0 2,944,022 + Dshi_2783 0.86 -0.3 2,944,077 + -0.4 2,944,118 - +0.6 2,946,637 + -1.2 2,946,670 - +1.0 2,946,684 + +0.6 2,946,696 + Dshi_2787 0.12 -1.1 2,946,798 - Dshi_2787 0.27 -0.5 2,946,812 + Dshi_2787 0.29 -0.3 2,946,812 + Dshi_2787 0.29 -0.5 2,946,820 - Dshi_2787 0.30 -0.1 2,946,874 + Dshi_2787 0.38 -0.5 2,946,948 - Dshi_2787 0.49 -0.8 2,946,960 + Dshi_2787 0.51 +0.4 2,946,983 + Dshi_2787 0.55 -0.3 2,947,010 + Dshi_2787 0.59 -0.8 2,947,068 + Dshi_2787 0.67 +1.3 2,947,075 + Dshi_2787 0.68 -0.6 2,947,083 - Dshi_2787 0.70 -0.2 2,947,201 + Dshi_2787 0.87 +1.4 2,947,231 - +0.5 2,947,243 - -0.9
Or see this region's nucleotide sequence