Experiment: Hans 100x vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_702 and Pf6N2E2_703 overlap by 4 nucleotides Pf6N2E2_703 and Pf6N2E2_704 are separated by 190 nucleotides Pf6N2E2_704 and Pf6N2E2_705 are separated by 24 nucleotides
Pf6N2E2_702 - Heme d1 biosynthesis protein NirF, at 807,015 to 808,193
_702
Pf6N2E2_703 - Cytochrome c55X precursor NirC, at 808,190 to 808,519
_703
Pf6N2E2_704 - Cytochrome c551/c552, at 808,710 to 809,018
_704
Pf6N2E2_705 - Cytochrome cd1 nitrite reductase (EC:1.7.2.1), at 809,043 to 810,722
_705
Position (kb) 808
809
810 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 807.814 kb on - strand, within Pf6N2E2_702 at 807.835 kb on + strand, within Pf6N2E2_702 at 807.970 kb on + strand, within Pf6N2E2_702 at 807.970 kb on + strand, within Pf6N2E2_702 at 807.970 kb on + strand, within Pf6N2E2_702 at 807.970 kb on + strand, within Pf6N2E2_702 at 807.970 kb on + strand, within Pf6N2E2_702 at 807.971 kb on - strand, within Pf6N2E2_702 at 807.971 kb on - strand, within Pf6N2E2_702 at 807.971 kb on - strand, within Pf6N2E2_702 at 807.971 kb on - strand, within Pf6N2E2_702 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.377 kb on + strand, within Pf6N2E2_703 at 808.378 kb on - strand, within Pf6N2E2_703 at 808.378 kb on - strand, within Pf6N2E2_703 at 808.534 kb on + strand at 808.534 kb on + strand at 808.535 kb on - strand at 808.711 kb on + strand at 808.712 kb on - strand at 808.712 kb on - strand at 808.712 kb on - strand at 808.715 kb on + strand at 808.716 kb on - strand at 808.716 kb on - strand at 808.805 kb on + strand, within Pf6N2E2_704 at 808.806 kb on - strand, within Pf6N2E2_704 at 808.806 kb on - strand, within Pf6N2E2_704 at 808.901 kb on + strand, within Pf6N2E2_704 at 808.902 kb on - strand, within Pf6N2E2_704 at 809.010 kb on - strand at 809.047 kb on + strand at 809.047 kb on + strand at 809.047 kb on + strand at 809.047 kb on + strand at 809.047 kb on + strand at 809.048 kb on - strand at 809.048 kb on - strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.068 kb on + strand at 809.069 kb on - strand at 809.218 kb on + strand, within Pf6N2E2_705 at 809.219 kb on - strand, within Pf6N2E2_705 at 809.263 kb on + strand, within Pf6N2E2_705 at 809.264 kb on - strand, within Pf6N2E2_705 at 809.368 kb on + strand, within Pf6N2E2_705 at 809.368 kb on + strand, within Pf6N2E2_705 at 809.369 kb on - strand, within Pf6N2E2_705 at 809.369 kb on - strand, within Pf6N2E2_705 at 809.369 kb on - strand, within Pf6N2E2_705 at 809.369 kb on - strand, within Pf6N2E2_705 at 809.369 kb on - strand, within Pf6N2E2_705 at 809.453 kb on - strand, within Pf6N2E2_705 at 809.453 kb on - strand, within Pf6N2E2_705 at 809.666 kb on - strand, within Pf6N2E2_705 at 809.666 kb on - strand, within Pf6N2E2_705 at 809.749 kb on + strand, within Pf6N2E2_705 at 809.959 kb on + strand, within Pf6N2E2_705 at 809.959 kb on + strand, within Pf6N2E2_705 at 809.959 kb on + strand, within Pf6N2E2_705 at 809.959 kb on + strand, within Pf6N2E2_705 at 809.960 kb on - strand, within Pf6N2E2_705
Per-strain Table
Position Strand Gene LocusTag Fraction Hans 100x vitamins remove 807,814 - Pf6N2E2_702 0.68 +0.5 807,835 + Pf6N2E2_702 0.70 +0.5 807,970 + Pf6N2E2_702 0.81 +0.3 807,970 + Pf6N2E2_702 0.81 +0.4 807,970 + Pf6N2E2_702 0.81 +0.2 807,970 + Pf6N2E2_702 0.81 +0.4 807,970 + Pf6N2E2_702 0.81 -0.4 807,971 - Pf6N2E2_702 0.81 -0.9 807,971 - Pf6N2E2_702 0.81 -0.6 807,971 - Pf6N2E2_702 0.81 -0.7 807,971 - Pf6N2E2_702 0.81 +0.5 808,377 + Pf6N2E2_703 0.57 +0.4 808,377 + Pf6N2E2_703 0.57 +0.2 808,377 + Pf6N2E2_703 0.57 +0.7 808,377 + Pf6N2E2_703 0.57 +0.0 808,377 + Pf6N2E2_703 0.57 +0.2 808,377 + Pf6N2E2_703 0.57 +0.5 808,378 - Pf6N2E2_703 0.57 -0.5 808,378 - Pf6N2E2_703 0.57 -1.3 808,534 + -1.3 808,534 + +0.4 808,535 - +1.1 808,711 + +0.2 808,712 - +0.1 808,712 - +0.3 808,712 - +0.2 808,715 + -0.1 808,716 - +1.2 808,716 - +3.1 808,805 + Pf6N2E2_704 0.31 -0.7 808,806 - Pf6N2E2_704 0.31 -1.0 808,806 - Pf6N2E2_704 0.31 -2.2 808,901 + Pf6N2E2_704 0.62 -0.0 808,902 - Pf6N2E2_704 0.62 -0.7 809,010 - +0.1 809,047 + -0.2 809,047 + -0.6 809,047 + +1.0 809,047 + +0.5 809,047 + +0.8 809,048 - +0.1 809,048 - +1.3 809,068 + +0.4 809,068 + +0.3 809,068 + -0.4 809,068 + +0.9 809,068 + -0.6 809,068 + -1.5 809,068 + +1.2 809,069 - +1.1 809,218 + Pf6N2E2_705 0.10 -1.0 809,219 - Pf6N2E2_705 0.10 -0.4 809,263 + Pf6N2E2_705 0.13 +0.3 809,264 - Pf6N2E2_705 0.13 +1.5 809,368 + Pf6N2E2_705 0.19 +0.1 809,368 + Pf6N2E2_705 0.19 +0.4 809,369 - Pf6N2E2_705 0.19 +0.7 809,369 - Pf6N2E2_705 0.19 +1.2 809,369 - Pf6N2E2_705 0.19 +0.4 809,369 - Pf6N2E2_705 0.19 +2.1 809,369 - Pf6N2E2_705 0.19 +1.4 809,453 - Pf6N2E2_705 0.24 +0.5 809,453 - Pf6N2E2_705 0.24 +0.6 809,666 - Pf6N2E2_705 0.37 +0.1 809,666 - Pf6N2E2_705 0.37 +0.9 809,749 + Pf6N2E2_705 0.42 +0.8 809,959 + Pf6N2E2_705 0.55 +0.2 809,959 + Pf6N2E2_705 0.55 +1.9 809,959 + Pf6N2E2_705 0.55 +0.8 809,959 + Pf6N2E2_705 0.55 -0.7 809,960 - Pf6N2E2_705 0.55 +1.9
Or see this region's nucleotide sequence