Experiment: Hans 100x vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6026 and Pf6N2E2_6027 are separated by 335 nucleotides Pf6N2E2_6027 and Pf6N2E2_6028 are separated by 339 nucleotides Pf6N2E2_6028 and Pf6N2E2_6029 are separated by 14 nucleotides Pf6N2E2_6029 and Pf6N2E2_6030 overlap by 10 nucleotides
Pf6N2E2_6026 - Probable serine protease do-like precursor (EC 3.4.21.-), at 6,843,859 to 6,844,998
_6026
Pf6N2E2_6027 - hypothetical protein, at 6,845,334 to 6,846,344
_6027
Pf6N2E2_6028 - hypothetical protein, at 6,846,684 to 6,846,821
_6028
Pf6N2E2_6029 - hypothetical protein, at 6,846,836 to 6,847,201
_6029
Pf6N2E2_6030 - Diaminopimelate epimerase (EC 5.1.1.7), at 6,847,192 to 6,847,995
_6030
Position (kb)
6845
6846
6847 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6844.360 kb on + strand, within Pf6N2E2_6026 at 6844.361 kb on - strand, within Pf6N2E2_6026 at 6844.366 kb on + strand, within Pf6N2E2_6026 at 6844.367 kb on - strand, within Pf6N2E2_6026 at 6844.367 kb on - strand, within Pf6N2E2_6026 at 6844.367 kb on - strand, within Pf6N2E2_6026 at 6844.369 kb on + strand, within Pf6N2E2_6026 at 6844.370 kb on - strand, within Pf6N2E2_6026 at 6844.370 kb on - strand, within Pf6N2E2_6026 at 6844.432 kb on - strand, within Pf6N2E2_6026 at 6844.793 kb on + strand, within Pf6N2E2_6026 at 6844.844 kb on + strand, within Pf6N2E2_6026 at 6844.844 kb on + strand, within Pf6N2E2_6026 at 6844.844 kb on + strand, within Pf6N2E2_6026 at 6844.844 kb on + strand, within Pf6N2E2_6026 at 6844.844 kb on + strand, within Pf6N2E2_6026 at 6844.844 kb on + strand, within Pf6N2E2_6026 at 6844.845 kb on - strand, within Pf6N2E2_6026 at 6844.845 kb on - strand, within Pf6N2E2_6026 at 6844.845 kb on - strand, within Pf6N2E2_6026 at 6844.845 kb on - strand, within Pf6N2E2_6026 at 6844.847 kb on + strand, within Pf6N2E2_6026 at 6844.848 kb on - strand, within Pf6N2E2_6026 at 6844.848 kb on - strand, within Pf6N2E2_6026 at 6844.848 kb on - strand, within Pf6N2E2_6026 at 6844.848 kb on - strand, within Pf6N2E2_6026 at 6844.848 kb on - strand, within Pf6N2E2_6026 at 6844.848 kb on - strand, within Pf6N2E2_6026 at 6844.878 kb on - strand, within Pf6N2E2_6026 at 6844.910 kb on + strand at 6844.910 kb on + strand at 6844.910 kb on + strand at 6844.911 kb on - strand at 6844.911 kb on - strand at 6844.911 kb on - strand at 6844.954 kb on + strand at 6844.954 kb on + strand at 6845.024 kb on + strand at 6845.025 kb on - strand at 6845.027 kb on + strand at 6845.027 kb on + strand at 6845.028 kb on - strand at 6845.028 kb on - strand at 6845.119 kb on + strand at 6845.119 kb on + strand at 6845.119 kb on + strand at 6845.119 kb on + strand at 6845.119 kb on + strand at 6845.120 kb on - strand at 6845.120 kb on - strand at 6845.120 kb on - strand at 6845.120 kb on - strand at 6845.127 kb on - strand at 6845.332 kb on + strand at 6845.332 kb on + strand at 6845.332 kb on + strand at 6845.367 kb on + strand at 6845.367 kb on + strand at 6845.367 kb on + strand at 6845.368 kb on - strand at 6845.368 kb on - strand at 6845.368 kb on - strand at 6845.517 kb on + strand, within Pf6N2E2_6027 at 6845.517 kb on + strand, within Pf6N2E2_6027 at 6845.517 kb on + strand, within Pf6N2E2_6027 at 6845.550 kb on + strand, within Pf6N2E2_6027 at 6845.550 kb on + strand, within Pf6N2E2_6027 at 6845.617 kb on + strand, within Pf6N2E2_6027 at 6845.618 kb on - strand, within Pf6N2E2_6027 at 6845.618 kb on - strand, within Pf6N2E2_6027 at 6845.618 kb on - strand, within Pf6N2E2_6027 at 6845.651 kb on - strand, within Pf6N2E2_6027 at 6845.890 kb on + strand, within Pf6N2E2_6027 at 6845.950 kb on + strand, within Pf6N2E2_6027 at 6845.950 kb on + strand, within Pf6N2E2_6027 at 6845.951 kb on - strand, within Pf6N2E2_6027 at 6845.983 kb on + strand, within Pf6N2E2_6027 at 6845.983 kb on + strand, within Pf6N2E2_6027 at 6845.984 kb on - strand, within Pf6N2E2_6027 at 6846.012 kb on + strand, within Pf6N2E2_6027 at 6846.012 kb on + strand, within Pf6N2E2_6027 at 6846.012 kb on + strand, within Pf6N2E2_6027 at 6846.012 kb on + strand, within Pf6N2E2_6027 at 6846.013 kb on - strand, within Pf6N2E2_6027 at 6846.013 kb on - strand, within Pf6N2E2_6027 at 6846.013 kb on - strand, within Pf6N2E2_6027 at 6846.026 kb on - strand, within Pf6N2E2_6027 at 6846.026 kb on - strand, within Pf6N2E2_6027 at 6846.026 kb on - strand, within Pf6N2E2_6027 at 6846.045 kb on + strand, within Pf6N2E2_6027 at 6846.046 kb on - strand, within Pf6N2E2_6027 at 6846.046 kb on - strand, within Pf6N2E2_6027 at 6846.117 kb on + strand, within Pf6N2E2_6027 at 6846.118 kb on - strand, within Pf6N2E2_6027 at 6846.170 kb on + strand, within Pf6N2E2_6027 at 6846.171 kb on - strand, within Pf6N2E2_6027 at 6846.461 kb on + strand at 6846.461 kb on + strand at 6846.462 kb on - strand at 6846.747 kb on + strand, within Pf6N2E2_6028 at 6846.866 kb on + strand at 6846.866 kb on + strand at 6846.866 kb on + strand at 6846.866 kb on + strand at 6846.866 kb on + strand at 6846.867 kb on - strand at 6846.867 kb on - strand at 6847.003 kb on + strand, within Pf6N2E2_6029 at 6847.004 kb on - strand, within Pf6N2E2_6029 at 6847.004 kb on - strand, within Pf6N2E2_6029 at 6847.013 kb on - strand, within Pf6N2E2_6029 at 6847.127 kb on + strand, within Pf6N2E2_6029
Per-strain Table
Position Strand Gene LocusTag Fraction Hans 100x vitamins remove 6,844,360 + Pf6N2E2_6026 0.44 -1.5 6,844,361 - Pf6N2E2_6026 0.44 +0.1 6,844,366 + Pf6N2E2_6026 0.44 +1.2 6,844,367 - Pf6N2E2_6026 0.45 +0.3 6,844,367 - Pf6N2E2_6026 0.45 +0.2 6,844,367 - Pf6N2E2_6026 0.45 +0.4 6,844,369 + Pf6N2E2_6026 0.45 -0.8 6,844,370 - Pf6N2E2_6026 0.45 +1.0 6,844,370 - Pf6N2E2_6026 0.45 -0.1 6,844,432 - Pf6N2E2_6026 0.50 +0.3 6,844,793 + Pf6N2E2_6026 0.82 -0.6 6,844,844 + Pf6N2E2_6026 0.86 +0.1 6,844,844 + Pf6N2E2_6026 0.86 +1.7 6,844,844 + Pf6N2E2_6026 0.86 +0.8 6,844,844 + Pf6N2E2_6026 0.86 -0.1 6,844,844 + Pf6N2E2_6026 0.86 +0.5 6,844,844 + Pf6N2E2_6026 0.86 -0.1 6,844,845 - Pf6N2E2_6026 0.86 +0.3 6,844,845 - Pf6N2E2_6026 0.86 -0.8 6,844,845 - Pf6N2E2_6026 0.86 +0.1 6,844,845 - Pf6N2E2_6026 0.86 -0.3 6,844,847 + Pf6N2E2_6026 0.87 -0.7 6,844,848 - Pf6N2E2_6026 0.87 -0.5 6,844,848 - Pf6N2E2_6026 0.87 +0.7 6,844,848 - Pf6N2E2_6026 0.87 +0.0 6,844,848 - Pf6N2E2_6026 0.87 -0.2 6,844,848 - Pf6N2E2_6026 0.87 -0.5 6,844,848 - Pf6N2E2_6026 0.87 +0.3 6,844,878 - Pf6N2E2_6026 0.89 +0.3 6,844,910 + +0.3 6,844,910 + +2.1 6,844,910 + +0.4 6,844,911 - -0.6 6,844,911 - -0.7 6,844,911 - +0.4 6,844,954 + +0.3 6,844,954 + +0.1 6,845,024 + +0.4 6,845,025 - +0.3 6,845,027 + -0.4 6,845,027 + -0.0 6,845,028 - +0.2 6,845,028 - -0.0 6,845,119 + -0.1 6,845,119 + +0.4 6,845,119 + -0.5 6,845,119 + +1.0 6,845,119 + +0.3 6,845,120 - +1.2 6,845,120 - -0.7 6,845,120 - +0.2 6,845,120 - +0.4 6,845,127 - +0.6 6,845,332 + -0.4 6,845,332 + -0.2 6,845,332 + +0.7 6,845,367 + -1.1 6,845,367 + +0.5 6,845,367 + +0.1 6,845,368 - +0.3 6,845,368 - +1.5 6,845,368 - +1.9 6,845,517 + Pf6N2E2_6027 0.18 +0.1 6,845,517 + Pf6N2E2_6027 0.18 -0.6 6,845,517 + Pf6N2E2_6027 0.18 -0.8 6,845,550 + Pf6N2E2_6027 0.21 +1.6 6,845,550 + Pf6N2E2_6027 0.21 +0.9 6,845,617 + Pf6N2E2_6027 0.28 +0.1 6,845,618 - Pf6N2E2_6027 0.28 -0.9 6,845,618 - Pf6N2E2_6027 0.28 -0.6 6,845,618 - Pf6N2E2_6027 0.28 -0.4 6,845,651 - Pf6N2E2_6027 0.31 +0.6 6,845,890 + Pf6N2E2_6027 0.55 -1.4 6,845,950 + Pf6N2E2_6027 0.61 -0.2 6,845,950 + Pf6N2E2_6027 0.61 -0.8 6,845,951 - Pf6N2E2_6027 0.61 -0.7 6,845,983 + Pf6N2E2_6027 0.64 -0.4 6,845,983 + Pf6N2E2_6027 0.64 +0.3 6,845,984 - Pf6N2E2_6027 0.64 -1.7 6,846,012 + Pf6N2E2_6027 0.67 -0.6 6,846,012 + Pf6N2E2_6027 0.67 +0.7 6,846,012 + Pf6N2E2_6027 0.67 -0.6 6,846,012 + Pf6N2E2_6027 0.67 +0.7 6,846,013 - Pf6N2E2_6027 0.67 +0.9 6,846,013 - Pf6N2E2_6027 0.67 -0.9 6,846,013 - Pf6N2E2_6027 0.67 +1.1 6,846,026 - Pf6N2E2_6027 0.68 -0.0 6,846,026 - Pf6N2E2_6027 0.68 +1.3 6,846,026 - Pf6N2E2_6027 0.68 -1.2 6,846,045 + Pf6N2E2_6027 0.70 -1.1 6,846,046 - Pf6N2E2_6027 0.70 -0.6 6,846,046 - Pf6N2E2_6027 0.70 +1.1 6,846,117 + Pf6N2E2_6027 0.77 -2.5 6,846,118 - Pf6N2E2_6027 0.78 +0.1 6,846,170 + Pf6N2E2_6027 0.83 +0.3 6,846,171 - Pf6N2E2_6027 0.83 -0.5 6,846,461 + -0.0 6,846,461 + -0.4 6,846,462 - -0.7 6,846,747 + Pf6N2E2_6028 0.46 +0.3 6,846,866 + -1.0 6,846,866 + -0.0 6,846,866 + -0.2 6,846,866 + +0.8 6,846,866 + -1.0 6,846,867 - +1.4 6,846,867 - +1.5 6,847,003 + Pf6N2E2_6029 0.46 -1.4 6,847,004 - Pf6N2E2_6029 0.46 +0.9 6,847,004 - Pf6N2E2_6029 0.46 -0.5 6,847,013 - Pf6N2E2_6029 0.48 +1.2 6,847,127 + Pf6N2E2_6029 0.80 +0.8
Or see this region's nucleotide sequence