Experiment: Hans 100x vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5937 and Pf6N2E2_5938 are separated by 267 nucleotides Pf6N2E2_5938 and Pf6N2E2_5939 overlap by 4 nucleotides
Pf6N2E2_5937 - UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158), at 6,755,854 to 6,756,873
_5937
Pf6N2E2_5938 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 6,757,141 to 6,757,611
_5938
Pf6N2E2_5939 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 6,757,608 to 6,759,923
_5939
Position (kb)
6757
6758 Strain fitness (log2 ratio)
-2
-1
0
1 at 6756.974 kb on + strand at 6756.975 kb on - strand at 6756.975 kb on - strand at 6757.040 kb on + strand at 6757.040 kb on + strand at 6757.040 kb on + strand at 6757.041 kb on - strand at 6757.041 kb on - strand at 6757.041 kb on - strand at 6757.041 kb on - strand at 6757.041 kb on - strand at 6757.041 kb on - strand at 6757.041 kb on - strand at 6757.114 kb on + strand at 6757.122 kb on + strand at 6757.123 kb on - strand at 6757.123 kb on - strand at 6757.123 kb on - strand at 6757.126 kb on + strand at 6757.126 kb on + strand at 6757.126 kb on + strand at 6757.126 kb on + strand at 6757.126 kb on + strand at 6757.126 kb on + strand at 6757.126 kb on + strand at 6757.127 kb on - strand at 6757.127 kb on - strand at 6757.435 kb on + strand, within Pf6N2E2_5938 at 6757.549 kb on + strand, within Pf6N2E2_5938 at 6757.549 kb on + strand, within Pf6N2E2_5938 at 6757.549 kb on + strand, within Pf6N2E2_5938 at 6757.549 kb on + strand, within Pf6N2E2_5938 at 6757.549 kb on + strand, within Pf6N2E2_5938 at 6757.549 kb on + strand, within Pf6N2E2_5938 at 6757.549 kb on + strand, within Pf6N2E2_5938 at 6757.550 kb on - strand, within Pf6N2E2_5938 at 6757.550 kb on - strand, within Pf6N2E2_5938 at 6757.550 kb on - strand, within Pf6N2E2_5938 at 6757.552 kb on - strand, within Pf6N2E2_5938 at 6757.792 kb on - strand at 6757.792 kb on - strand at 6757.792 kb on - strand at 6757.792 kb on - strand at 6757.862 kb on + strand, within Pf6N2E2_5939 at 6757.862 kb on + strand, within Pf6N2E2_5939 at 6757.915 kb on + strand, within Pf6N2E2_5939 at 6757.916 kb on - strand, within Pf6N2E2_5939 at 6758.136 kb on + strand, within Pf6N2E2_5939 at 6758.221 kb on - strand, within Pf6N2E2_5939 at 6758.316 kb on + strand, within Pf6N2E2_5939 at 6758.316 kb on + strand, within Pf6N2E2_5939 at 6758.317 kb on - strand, within Pf6N2E2_5939 at 6758.317 kb on - strand, within Pf6N2E2_5939 at 6758.317 kb on - strand, within Pf6N2E2_5939 at 6758.343 kb on + strand, within Pf6N2E2_5939 at 6758.344 kb on - strand, within Pf6N2E2_5939 at 6758.344 kb on - strand, within Pf6N2E2_5939 at 6758.389 kb on + strand, within Pf6N2E2_5939 at 6758.390 kb on - strand, within Pf6N2E2_5939
Per-strain Table
Position Strand Gene LocusTag Fraction Hans 100x vitamins remove 6,756,974 + +0.5 6,756,975 - -0.0 6,756,975 - -0.5 6,757,040 + -0.4 6,757,040 + +1.6 6,757,040 + +1.3 6,757,041 - +0.0 6,757,041 - -0.2 6,757,041 - +0.1 6,757,041 - -0.0 6,757,041 - +0.3 6,757,041 - -0.0 6,757,041 - +0.1 6,757,114 + +1.3 6,757,122 + -0.6 6,757,123 - +0.7 6,757,123 - -0.3 6,757,123 - +0.4 6,757,126 + +0.2 6,757,126 + +0.1 6,757,126 + +0.7 6,757,126 + -0.5 6,757,126 + -1.7 6,757,126 + +0.1 6,757,126 + +0.8 6,757,127 - +1.0 6,757,127 - -0.0 6,757,435 + Pf6N2E2_5938 0.62 -2.2 6,757,549 + Pf6N2E2_5938 0.87 -0.0 6,757,549 + Pf6N2E2_5938 0.87 -0.1 6,757,549 + Pf6N2E2_5938 0.87 +0.5 6,757,549 + Pf6N2E2_5938 0.87 -0.6 6,757,549 + Pf6N2E2_5938 0.87 -0.7 6,757,549 + Pf6N2E2_5938 0.87 -0.6 6,757,549 + Pf6N2E2_5938 0.87 -0.3 6,757,550 - Pf6N2E2_5938 0.87 -0.4 6,757,550 - Pf6N2E2_5938 0.87 -0.6 6,757,550 - Pf6N2E2_5938 0.87 -0.8 6,757,552 - Pf6N2E2_5938 0.87 -2.5 6,757,792 - +0.2 6,757,792 - -0.9 6,757,792 - +0.9 6,757,792 - +0.8 6,757,862 + Pf6N2E2_5939 0.11 -1.6 6,757,862 + Pf6N2E2_5939 0.11 -0.1 6,757,915 + Pf6N2E2_5939 0.13 +0.7 6,757,916 - Pf6N2E2_5939 0.13 +0.3 6,758,136 + Pf6N2E2_5939 0.23 -0.6 6,758,221 - Pf6N2E2_5939 0.26 -0.0 6,758,316 + Pf6N2E2_5939 0.31 -1.5 6,758,316 + Pf6N2E2_5939 0.31 -0.0 6,758,317 - Pf6N2E2_5939 0.31 -0.1 6,758,317 - Pf6N2E2_5939 0.31 +0.4 6,758,317 - Pf6N2E2_5939 0.31 -0.7 6,758,343 + Pf6N2E2_5939 0.32 +0.2 6,758,344 - Pf6N2E2_5939 0.32 +0.3 6,758,344 - Pf6N2E2_5939 0.32 -1.2 6,758,389 + Pf6N2E2_5939 0.34 +0.6 6,758,390 - Pf6N2E2_5939 0.34 +0.5
Or see this region's nucleotide sequence