Experiment: Hans 100x vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3160 and Pf6N2E2_3161 overlap by 21 nucleotides Pf6N2E2_3161 and Pf6N2E2_3162 are separated by 73 nucleotides Pf6N2E2_3162 and Pf6N2E2_3163 are separated by 181 nucleotides
Pf6N2E2_3160 - Multicopper oxidase, at 3,654,154 to 3,655,530
_3160
Pf6N2E2_3161 - hypothetical protein, at 3,655,510 to 3,655,653
_3161
Pf6N2E2_3162 - hypothetical protein, at 3,655,727 to 3,656,119
_3162
Pf6N2E2_3163 - putative DNA-binding protein, at 3,656,301 to 3,656,825
_3163
Position (kb)
3655
3656 Strain fitness (log2 ratio)
-2
-1
0
1 at 3654.747 kb on - strand, within Pf6N2E2_3160 at 3654.795 kb on - strand, within Pf6N2E2_3160 at 3654.795 kb on - strand, within Pf6N2E2_3160 at 3654.795 kb on - strand, within Pf6N2E2_3160 at 3654.795 kb on - strand, within Pf6N2E2_3160 at 3654.826 kb on - strand, within Pf6N2E2_3160 at 3654.826 kb on - strand, within Pf6N2E2_3160 at 3654.831 kb on - strand, within Pf6N2E2_3160 at 3654.831 kb on - strand, within Pf6N2E2_3160 at 3654.831 kb on - strand, within Pf6N2E2_3160 at 3654.831 kb on - strand, within Pf6N2E2_3160 at 3654.831 kb on - strand, within Pf6N2E2_3160 at 3655.032 kb on - strand, within Pf6N2E2_3160 at 3655.032 kb on - strand, within Pf6N2E2_3160 at 3655.032 kb on - strand, within Pf6N2E2_3160 at 3655.032 kb on - strand, within Pf6N2E2_3160 at 3655.032 kb on - strand, within Pf6N2E2_3160 at 3655.122 kb on - strand, within Pf6N2E2_3160 at 3655.167 kb on - strand, within Pf6N2E2_3160 at 3655.200 kb on - strand, within Pf6N2E2_3160 at 3655.200 kb on - strand, within Pf6N2E2_3160 at 3655.200 kb on - strand, within Pf6N2E2_3160 at 3655.200 kb on - strand, within Pf6N2E2_3160 at 3655.407 kb on - strand at 3655.407 kb on - strand at 3655.545 kb on - strand, within Pf6N2E2_3161 at 3655.545 kb on - strand, within Pf6N2E2_3161 at 3655.545 kb on - strand, within Pf6N2E2_3161 at 3655.590 kb on - strand, within Pf6N2E2_3161 at 3655.612 kb on - strand, within Pf6N2E2_3161 at 3655.960 kb on + strand, within Pf6N2E2_3162 at 3655.962 kb on + strand, within Pf6N2E2_3162 at 3655.962 kb on + strand, within Pf6N2E2_3162 at 3655.963 kb on - strand, within Pf6N2E2_3162 at 3655.963 kb on - strand, within Pf6N2E2_3162 at 3655.986 kb on + strand, within Pf6N2E2_3162 at 3656.069 kb on + strand, within Pf6N2E2_3162 at 3656.069 kb on + strand, within Pf6N2E2_3162 at 3656.070 kb on - strand, within Pf6N2E2_3162 at 3656.169 kb on + strand at 3656.170 kb on - strand at 3656.171 kb on + strand at 3656.171 kb on + strand at 3656.172 kb on - strand at 3656.172 kb on - strand at 3656.192 kb on - strand at 3656.192 kb on - strand at 3656.192 kb on - strand at 3656.192 kb on - strand at 3656.199 kb on + strand at 3656.257 kb on - strand at 3656.411 kb on + strand, within Pf6N2E2_3163 at 3656.419 kb on + strand, within Pf6N2E2_3163 at 3656.419 kb on + strand, within Pf6N2E2_3163 at 3656.420 kb on - strand, within Pf6N2E2_3163 at 3656.469 kb on - strand, within Pf6N2E2_3163 at 3656.469 kb on - strand, within Pf6N2E2_3163 at 3656.469 kb on - strand, within Pf6N2E2_3163 at 3656.548 kb on - strand, within Pf6N2E2_3163 at 3656.548 kb on - strand, within Pf6N2E2_3163 at 3656.548 kb on - strand, within Pf6N2E2_3163 at 3656.565 kb on + strand, within Pf6N2E2_3163 at 3656.583 kb on + strand, within Pf6N2E2_3163 at 3656.584 kb on - strand, within Pf6N2E2_3163 at 3656.584 kb on - strand, within Pf6N2E2_3163 at 3656.596 kb on - strand, within Pf6N2E2_3163 at 3656.648 kb on + strand, within Pf6N2E2_3163 at 3656.648 kb on + strand, within Pf6N2E2_3163 at 3656.648 kb on + strand, within Pf6N2E2_3163 at 3656.649 kb on - strand, within Pf6N2E2_3163
Per-strain Table
Position Strand Gene LocusTag Fraction Hans 100x vitamins remove 3,654,747 - Pf6N2E2_3160 0.43 -0.2 3,654,795 - Pf6N2E2_3160 0.47 -0.3 3,654,795 - Pf6N2E2_3160 0.47 -0.0 3,654,795 - Pf6N2E2_3160 0.47 -0.6 3,654,795 - Pf6N2E2_3160 0.47 +1.0 3,654,826 - Pf6N2E2_3160 0.49 +0.3 3,654,826 - Pf6N2E2_3160 0.49 +1.7 3,654,831 - Pf6N2E2_3160 0.49 +1.4 3,654,831 - Pf6N2E2_3160 0.49 -1.6 3,654,831 - Pf6N2E2_3160 0.49 +0.2 3,654,831 - Pf6N2E2_3160 0.49 -0.4 3,654,831 - Pf6N2E2_3160 0.49 -0.4 3,655,032 - Pf6N2E2_3160 0.64 +1.6 3,655,032 - Pf6N2E2_3160 0.64 -0.2 3,655,032 - Pf6N2E2_3160 0.64 +1.0 3,655,032 - Pf6N2E2_3160 0.64 +0.2 3,655,032 - Pf6N2E2_3160 0.64 +0.9 3,655,122 - Pf6N2E2_3160 0.70 -0.3 3,655,167 - Pf6N2E2_3160 0.74 +0.1 3,655,200 - Pf6N2E2_3160 0.76 -0.0 3,655,200 - Pf6N2E2_3160 0.76 -0.4 3,655,200 - Pf6N2E2_3160 0.76 -0.7 3,655,200 - Pf6N2E2_3160 0.76 +0.6 3,655,407 - +0.4 3,655,407 - -1.0 3,655,545 - Pf6N2E2_3161 0.24 -0.9 3,655,545 - Pf6N2E2_3161 0.24 +0.9 3,655,545 - Pf6N2E2_3161 0.24 -0.8 3,655,590 - Pf6N2E2_3161 0.56 -1.6 3,655,612 - Pf6N2E2_3161 0.71 -2.1 3,655,960 + Pf6N2E2_3162 0.59 -0.1 3,655,962 + Pf6N2E2_3162 0.60 +1.1 3,655,962 + Pf6N2E2_3162 0.60 -0.0 3,655,963 - Pf6N2E2_3162 0.60 +1.3 3,655,963 - Pf6N2E2_3162 0.60 -0.2 3,655,986 + Pf6N2E2_3162 0.66 -1.2 3,656,069 + Pf6N2E2_3162 0.87 +0.5 3,656,069 + Pf6N2E2_3162 0.87 +0.9 3,656,070 - Pf6N2E2_3162 0.87 +0.5 3,656,169 + -0.6 3,656,170 - -0.4 3,656,171 + +0.9 3,656,171 + -0.8 3,656,172 - -0.3 3,656,172 - +0.5 3,656,192 - -0.6 3,656,192 - -0.2 3,656,192 - +0.7 3,656,192 - +0.6 3,656,199 + -0.6 3,656,257 - -1.0 3,656,411 + Pf6N2E2_3163 0.21 +1.3 3,656,419 + Pf6N2E2_3163 0.22 +0.0 3,656,419 + Pf6N2E2_3163 0.22 -1.0 3,656,420 - Pf6N2E2_3163 0.23 +0.9 3,656,469 - Pf6N2E2_3163 0.32 +0.5 3,656,469 - Pf6N2E2_3163 0.32 -0.7 3,656,469 - Pf6N2E2_3163 0.32 +1.2 3,656,548 - Pf6N2E2_3163 0.47 -0.5 3,656,548 - Pf6N2E2_3163 0.47 +0.5 3,656,548 - Pf6N2E2_3163 0.47 +0.6 3,656,565 + Pf6N2E2_3163 0.50 -0.4 3,656,583 + Pf6N2E2_3163 0.54 +0.6 3,656,584 - Pf6N2E2_3163 0.54 +0.2 3,656,584 - Pf6N2E2_3163 0.54 -0.7 3,656,596 - Pf6N2E2_3163 0.56 +0.9 3,656,648 + Pf6N2E2_3163 0.66 -0.0 3,656,648 + Pf6N2E2_3163 0.66 -1.2 3,656,648 + Pf6N2E2_3163 0.66 -1.6 3,656,649 - Pf6N2E2_3163 0.66 -1.2
Or see this region's nucleotide sequence