Experiment: Hans 100x vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2858 and Pf6N2E2_2859 are separated by 11 nucleotides Pf6N2E2_2859 and Pf6N2E2_2860 are separated by 80 nucleotides Pf6N2E2_2860 and Pf6N2E2_2861 are separated by 32 nucleotides Pf6N2E2_2861 and Pf6N2E2_2862 overlap by 12 nucleotides
Pf6N2E2_2858 - Phosphohydrolase (MutT/nudix family protein), at 3,349,269 to 3,349,877
_2858
Pf6N2E2_2859 - Bacterial transcription activator, effector binding protein, at 3,349,889 to 3,350,377
_2859
Pf6N2E2_2860 - Putative threonine efflux protein, at 3,350,458 to 3,351,120
_2860
Pf6N2E2_2861 - hypothetical protein, at 3,351,153 to 3,351,341
_2861
Pf6N2E2_2862 - Uncharacterized PLP-dependent aminotransferase YfdZ, at 3,351,330 to 3,352,547
_2862
Position (kb)
3350
3351
3352 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3349.833 kb on + strand at 3349.833 kb on + strand at 3349.834 kb on - strand at 3349.834 kb on - strand at 3349.873 kb on - strand at 3349.873 kb on - strand at 3349.959 kb on + strand, within Pf6N2E2_2859 at 3349.990 kb on + strand, within Pf6N2E2_2859 at 3349.991 kb on - strand, within Pf6N2E2_2859 at 3349.991 kb on - strand, within Pf6N2E2_2859 at 3349.991 kb on - strand, within Pf6N2E2_2859 at 3350.025 kb on + strand, within Pf6N2E2_2859 at 3350.025 kb on + strand, within Pf6N2E2_2859 at 3350.025 kb on + strand, within Pf6N2E2_2859 at 3350.025 kb on + strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.043 kb on - strand, within Pf6N2E2_2859 at 3350.080 kb on - strand, within Pf6N2E2_2859 at 3350.082 kb on + strand, within Pf6N2E2_2859 at 3350.157 kb on + strand, within Pf6N2E2_2859 at 3350.157 kb on + strand, within Pf6N2E2_2859 at 3350.158 kb on - strand, within Pf6N2E2_2859 at 3350.199 kb on + strand, within Pf6N2E2_2859 at 3350.200 kb on - strand, within Pf6N2E2_2859 at 3350.200 kb on - strand, within Pf6N2E2_2859 at 3350.200 kb on - strand, within Pf6N2E2_2859 at 3350.200 kb on - strand, within Pf6N2E2_2859 at 3350.314 kb on + strand, within Pf6N2E2_2859 at 3350.355 kb on + strand at 3350.420 kb on + strand at 3350.420 kb on + strand at 3350.420 kb on + strand at 3350.421 kb on - strand at 3350.421 kb on - strand at 3350.421 kb on - strand at 3350.421 kb on - strand at 3350.421 kb on - strand at 3350.421 kb on - strand at 3350.494 kb on + strand at 3350.526 kb on - strand, within Pf6N2E2_2860 at 3350.712 kb on + strand, within Pf6N2E2_2860 at 3350.713 kb on - strand, within Pf6N2E2_2860 at 3350.718 kb on + strand, within Pf6N2E2_2860 at 3350.847 kb on - strand, within Pf6N2E2_2860 at 3350.879 kb on + strand, within Pf6N2E2_2860 at 3350.879 kb on + strand, within Pf6N2E2_2860 at 3350.880 kb on - strand, within Pf6N2E2_2860 at 3350.930 kb on + strand, within Pf6N2E2_2860 at 3350.931 kb on - strand, within Pf6N2E2_2860 at 3350.973 kb on - strand, within Pf6N2E2_2860 at 3351.070 kb on + strand at 3351.070 kb on + strand at 3351.070 kb on + strand at 3351.071 kb on - strand at 3351.071 kb on - strand at 3351.071 kb on - strand at 3351.121 kb on + strand at 3351.122 kb on - strand at 3351.143 kb on + strand at 3351.213 kb on + strand, within Pf6N2E2_2861 at 3351.255 kb on - strand, within Pf6N2E2_2861 at 3351.315 kb on + strand, within Pf6N2E2_2861 at 3351.315 kb on + strand, within Pf6N2E2_2861 at 3351.315 kb on + strand, within Pf6N2E2_2861 at 3351.316 kb on - strand, within Pf6N2E2_2861 at 3351.316 kb on - strand, within Pf6N2E2_2861 at 3351.316 kb on - strand, within Pf6N2E2_2861 at 3351.316 kb on - strand, within Pf6N2E2_2861 at 3351.316 kb on - strand, within Pf6N2E2_2861 at 3351.384 kb on + strand at 3351.385 kb on - strand at 3351.388 kb on + strand at 3351.388 kb on + strand at 3351.388 kb on + strand at 3351.389 kb on - strand at 3351.389 kb on - strand at 3351.389 kb on - strand at 3351.396 kb on - strand at 3351.589 kb on - strand, within Pf6N2E2_2862 at 3351.601 kb on - strand, within Pf6N2E2_2862 at 3351.601 kb on - strand, within Pf6N2E2_2862 at 3351.738 kb on + strand, within Pf6N2E2_2862 at 3351.739 kb on - strand, within Pf6N2E2_2862 at 3351.739 kb on - strand, within Pf6N2E2_2862 at 3351.739 kb on - strand, within Pf6N2E2_2862 at 3351.936 kb on + strand, within Pf6N2E2_2862 at 3351.937 kb on - strand, within Pf6N2E2_2862 at 3352.065 kb on + strand, within Pf6N2E2_2862 at 3352.065 kb on + strand, within Pf6N2E2_2862 at 3352.066 kb on - strand, within Pf6N2E2_2862 at 3352.066 kb on - strand, within Pf6N2E2_2862
Per-strain Table
Position Strand Gene LocusTag Fraction Hans 100x vitamins remove 3,349,833 + -0.0 3,349,833 + -1.1 3,349,834 - +0.9 3,349,834 - +0.6 3,349,873 - +0.5 3,349,873 - +0.1 3,349,959 + Pf6N2E2_2859 0.14 +0.4 3,349,990 + Pf6N2E2_2859 0.21 -0.4 3,349,991 - Pf6N2E2_2859 0.21 +1.9 3,349,991 - Pf6N2E2_2859 0.21 +0.8 3,349,991 - Pf6N2E2_2859 0.21 +0.3 3,350,025 + Pf6N2E2_2859 0.28 +0.9 3,350,025 + Pf6N2E2_2859 0.28 -1.1 3,350,025 + Pf6N2E2_2859 0.28 +0.1 3,350,025 + Pf6N2E2_2859 0.28 +0.3 3,350,026 - Pf6N2E2_2859 0.28 -0.8 3,350,026 - Pf6N2E2_2859 0.28 -0.0 3,350,026 - Pf6N2E2_2859 0.28 +0.5 3,350,026 - Pf6N2E2_2859 0.28 -0.7 3,350,026 - Pf6N2E2_2859 0.28 -0.2 3,350,026 - Pf6N2E2_2859 0.28 -0.4 3,350,043 - Pf6N2E2_2859 0.31 -0.4 3,350,080 - Pf6N2E2_2859 0.39 +0.0 3,350,082 + Pf6N2E2_2859 0.39 +0.1 3,350,157 + Pf6N2E2_2859 0.55 -0.0 3,350,157 + Pf6N2E2_2859 0.55 -0.5 3,350,158 - Pf6N2E2_2859 0.55 +0.3 3,350,199 + Pf6N2E2_2859 0.63 +0.4 3,350,200 - Pf6N2E2_2859 0.64 -1.2 3,350,200 - Pf6N2E2_2859 0.64 -0.4 3,350,200 - Pf6N2E2_2859 0.64 -1.1 3,350,200 - Pf6N2E2_2859 0.64 -0.2 3,350,314 + Pf6N2E2_2859 0.87 -0.3 3,350,355 + +0.0 3,350,420 + +1.4 3,350,420 + -0.5 3,350,420 + +0.5 3,350,421 - +0.8 3,350,421 - -0.7 3,350,421 - -1.5 3,350,421 - +0.0 3,350,421 - +1.8 3,350,421 - -0.6 3,350,494 + -0.1 3,350,526 - Pf6N2E2_2860 0.10 -1.0 3,350,712 + Pf6N2E2_2860 0.38 +0.4 3,350,713 - Pf6N2E2_2860 0.38 -0.6 3,350,718 + Pf6N2E2_2860 0.39 -1.4 3,350,847 - Pf6N2E2_2860 0.59 -1.4 3,350,879 + Pf6N2E2_2860 0.63 -1.3 3,350,879 + Pf6N2E2_2860 0.63 -0.3 3,350,880 - Pf6N2E2_2860 0.64 +0.0 3,350,930 + Pf6N2E2_2860 0.71 +1.0 3,350,931 - Pf6N2E2_2860 0.71 -0.7 3,350,973 - Pf6N2E2_2860 0.78 -1.4 3,351,070 + -0.3 3,351,070 + -0.5 3,351,070 + -1.0 3,351,071 - +0.8 3,351,071 - -0.1 3,351,071 - -0.5 3,351,121 + +0.3 3,351,122 - +0.1 3,351,143 + +0.6 3,351,213 + Pf6N2E2_2861 0.32 -0.5 3,351,255 - Pf6N2E2_2861 0.54 -0.7 3,351,315 + Pf6N2E2_2861 0.86 +0.4 3,351,315 + Pf6N2E2_2861 0.86 -0.8 3,351,315 + Pf6N2E2_2861 0.86 +0.3 3,351,316 - Pf6N2E2_2861 0.86 +1.0 3,351,316 - Pf6N2E2_2861 0.86 -1.9 3,351,316 - Pf6N2E2_2861 0.86 +1.0 3,351,316 - Pf6N2E2_2861 0.86 +0.4 3,351,316 - Pf6N2E2_2861 0.86 +0.5 3,351,384 + +0.9 3,351,385 - +0.3 3,351,388 + +0.6 3,351,388 + -0.3 3,351,388 + -0.1 3,351,389 - +0.7 3,351,389 - +1.7 3,351,389 - +0.0 3,351,396 - -1.8 3,351,589 - Pf6N2E2_2862 0.21 +0.4 3,351,601 - Pf6N2E2_2862 0.22 +1.8 3,351,601 - Pf6N2E2_2862 0.22 +1.0 3,351,738 + Pf6N2E2_2862 0.33 -1.0 3,351,739 - Pf6N2E2_2862 0.34 +0.4 3,351,739 - Pf6N2E2_2862 0.34 -0.7 3,351,739 - Pf6N2E2_2862 0.34 +0.7 3,351,936 + Pf6N2E2_2862 0.50 -0.8 3,351,937 - Pf6N2E2_2862 0.50 -1.0 3,352,065 + Pf6N2E2_2862 0.60 -0.8 3,352,065 + Pf6N2E2_2862 0.60 +0.5 3,352,066 - Pf6N2E2_2862 0.60 -2.1 3,352,066 - Pf6N2E2_2862 0.60 +0.4
Or see this region's nucleotide sequence