Experiment: Hans 100x vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_284 and Pf6N2E2_285 are separated by 248 nucleotides Pf6N2E2_285 and Pf6N2E2_286 overlap by 4 nucleotides
Pf6N2E2_284 - Adenylosuccinate lyase (EC 4.3.2.2), at 350,256 to 351,626
_284
Pf6N2E2_285 - FIG002903: a protein of unknown function perhaps involved in purine metabolism, at 351,875 to 352,498
_285
Pf6N2E2_286 - tRNA-specific 2-thiouridylase MnmA, at 352,495 to 353,619
_286
Position (kb) 351
352
353 Strain fitness (log2 ratio) -1
0
1 at 351.713 kb on - strand at 351.732 kb on + strand at 351.732 kb on + strand at 351.732 kb on + strand at 351.732 kb on + strand at 351.733 kb on - strand at 351.733 kb on - strand at 351.733 kb on - strand at 351.733 kb on - strand at 351.733 kb on - strand at 351.733 kb on - strand at 351.733 kb on - strand at 351.736 kb on + strand at 351.736 kb on + strand at 351.736 kb on + strand at 351.737 kb on - strand at 351.737 kb on - strand at 351.737 kb on - strand at 351.737 kb on - strand at 351.739 kb on + strand at 351.739 kb on + strand at 351.740 kb on - strand at 351.876 kb on + strand at 351.876 kb on + strand at 351.877 kb on - strand at 352.075 kb on + strand, within Pf6N2E2_285 at 352.093 kb on - strand, within Pf6N2E2_285 at 352.242 kb on + strand, within Pf6N2E2_285 at 352.306 kb on - strand, within Pf6N2E2_285 at 352.338 kb on + strand, within Pf6N2E2_285 at 352.339 kb on - strand, within Pf6N2E2_285 at 352.394 kb on + strand, within Pf6N2E2_285
Per-strain Table
Position Strand Gene LocusTag Fraction Hans 100x vitamins remove 351,713 - -0.5 351,732 + +0.3 351,732 + -0.5 351,732 + +0.1 351,732 + +1.6 351,733 - +0.2 351,733 - -0.1 351,733 - +0.0 351,733 - -0.9 351,733 - +0.6 351,733 - +0.2 351,733 - +0.7 351,736 + -0.8 351,736 + +1.5 351,736 + -1.4 351,737 - +0.4 351,737 - +1.7 351,737 - +0.1 351,737 - +0.4 351,739 + +0.2 351,739 + -1.3 351,740 - -0.6 351,876 + +0.9 351,876 + +0.7 351,877 - +0.7 352,075 + Pf6N2E2_285 0.32 -1.0 352,093 - Pf6N2E2_285 0.35 -0.5 352,242 + Pf6N2E2_285 0.59 -1.6 352,306 - Pf6N2E2_285 0.69 +0.0 352,338 + Pf6N2E2_285 0.74 +0.5 352,339 - Pf6N2E2_285 0.74 -1.1 352,394 + Pf6N2E2_285 0.83 -1.0
Or see this region's nucleotide sequence