Experiment: Hans 100x vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2113 and Pf6N2E2_2114 are separated by 688 nucleotides Pf6N2E2_2114 and Pf6N2E2_2115 are separated by 244 nucleotides
Pf6N2E2_2113 - Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 2,509,522 to 2,510,703
_2113
Pf6N2E2_2114 - Predicted D-glucarate or D-galactorate regulator, GntR family, at 2,511,392 to 2,512,102
_2114
Pf6N2E2_2115 - Major facilitator family transporter, at 2,512,347 to 2,513,789
_2115
Position (kb)
2511
2512
2513 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2511.387 kb on - strand at 2511.387 kb on - strand at 2511.702 kb on + strand, within Pf6N2E2_2114 at 2511.947 kb on - strand, within Pf6N2E2_2114 at 2511.947 kb on - strand, within Pf6N2E2_2114 at 2512.138 kb on - strand at 2512.183 kb on - strand at 2512.265 kb on - strand at 2512.554 kb on + strand, within Pf6N2E2_2115 at 2512.555 kb on - strand, within Pf6N2E2_2115 at 2512.555 kb on - strand, within Pf6N2E2_2115 at 2512.633 kb on + strand, within Pf6N2E2_2115 at 2512.633 kb on + strand, within Pf6N2E2_2115 at 2512.633 kb on + strand, within Pf6N2E2_2115 at 2512.633 kb on + strand, within Pf6N2E2_2115 at 2512.633 kb on + strand, within Pf6N2E2_2115 at 2512.634 kb on - strand, within Pf6N2E2_2115 at 2512.660 kb on - strand, within Pf6N2E2_2115 at 2512.712 kb on + strand, within Pf6N2E2_2115 at 2512.791 kb on + strand, within Pf6N2E2_2115 at 2512.792 kb on - strand, within Pf6N2E2_2115 at 2512.792 kb on - strand, within Pf6N2E2_2115 at 2512.792 kb on - strand, within Pf6N2E2_2115 at 2512.792 kb on - strand, within Pf6N2E2_2115 at 2512.792 kb on - strand, within Pf6N2E2_2115 at 2512.848 kb on + strand, within Pf6N2E2_2115 at 2512.848 kb on + strand, within Pf6N2E2_2115 at 2512.848 kb on + strand, within Pf6N2E2_2115 at 2512.849 kb on - strand, within Pf6N2E2_2115 at 2512.849 kb on - strand, within Pf6N2E2_2115 at 2512.849 kb on - strand, within Pf6N2E2_2115 at 2512.849 kb on - strand, within Pf6N2E2_2115 at 2512.852 kb on - strand, within Pf6N2E2_2115 at 2512.852 kb on - strand, within Pf6N2E2_2115 at 2512.852 kb on - strand, within Pf6N2E2_2115 at 2512.852 kb on - strand, within Pf6N2E2_2115 at 2512.852 kb on - strand, within Pf6N2E2_2115 at 2512.852 kb on - strand, within Pf6N2E2_2115 at 2513.001 kb on + strand, within Pf6N2E2_2115 at 2513.002 kb on - strand, within Pf6N2E2_2115 at 2513.002 kb on - strand, within Pf6N2E2_2115 at 2513.002 kb on - strand, within Pf6N2E2_2115
Per-strain Table
Position Strand Gene LocusTag Fraction Hans 100x vitamins remove 2,511,387 - +0.1 2,511,387 - -0.4 2,511,702 + Pf6N2E2_2114 0.44 -1.2 2,511,947 - Pf6N2E2_2114 0.78 -0.9 2,511,947 - Pf6N2E2_2114 0.78 -0.5 2,512,138 - -0.8 2,512,183 - -0.0 2,512,265 - -0.9 2,512,554 + Pf6N2E2_2115 0.14 +0.1 2,512,555 - Pf6N2E2_2115 0.14 -0.3 2,512,555 - Pf6N2E2_2115 0.14 +1.5 2,512,633 + Pf6N2E2_2115 0.20 +1.1 2,512,633 + Pf6N2E2_2115 0.20 -0.6 2,512,633 + Pf6N2E2_2115 0.20 +0.6 2,512,633 + Pf6N2E2_2115 0.20 -0.1 2,512,633 + Pf6N2E2_2115 0.20 -0.1 2,512,634 - Pf6N2E2_2115 0.20 -1.5 2,512,660 - Pf6N2E2_2115 0.22 -0.0 2,512,712 + Pf6N2E2_2115 0.25 -0.5 2,512,791 + Pf6N2E2_2115 0.31 -0.3 2,512,792 - Pf6N2E2_2115 0.31 +0.4 2,512,792 - Pf6N2E2_2115 0.31 -0.8 2,512,792 - Pf6N2E2_2115 0.31 +0.3 2,512,792 - Pf6N2E2_2115 0.31 -1.9 2,512,792 - Pf6N2E2_2115 0.31 +1.1 2,512,848 + Pf6N2E2_2115 0.35 +2.1 2,512,848 + Pf6N2E2_2115 0.35 -0.3 2,512,848 + Pf6N2E2_2115 0.35 +1.7 2,512,849 - Pf6N2E2_2115 0.35 -0.3 2,512,849 - Pf6N2E2_2115 0.35 +0.2 2,512,849 - Pf6N2E2_2115 0.35 -0.5 2,512,849 - Pf6N2E2_2115 0.35 -0.1 2,512,852 - Pf6N2E2_2115 0.35 +1.1 2,512,852 - Pf6N2E2_2115 0.35 +0.4 2,512,852 - Pf6N2E2_2115 0.35 -0.5 2,512,852 - Pf6N2E2_2115 0.35 +1.4 2,512,852 - Pf6N2E2_2115 0.35 -1.7 2,512,852 - Pf6N2E2_2115 0.35 -0.4 2,513,001 + Pf6N2E2_2115 0.45 +1.0 2,513,002 - Pf6N2E2_2115 0.45 +0.1 2,513,002 - Pf6N2E2_2115 0.45 -0.1 2,513,002 - Pf6N2E2_2115 0.45 -1.8
Or see this region's nucleotide sequence