Experiment: Hans 100x vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1806 and Pf6N2E2_1807 are separated by 58 nucleotides Pf6N2E2_1807 and Pf6N2E2_1808 are separated by 54 nucleotides
Pf6N2E2_1806 - hypothetical protein, at 2,136,731 to 2,137,237
_1806
Pf6N2E2_1807 - hypothetical protein, at 2,137,296 to 2,139,569
_1807
Pf6N2E2_1808 - Transcriptional regulator, at 2,139,624 to 2,140,538
_1808
Position (kb)
2137
2138
2139
2140 Strain fitness (log2 ratio)
-2
-1
0
1 at 2136.384 kb on + strand at 2136.385 kb on - strand at 2136.758 kb on + strand at 2136.921 kb on - strand, within Pf6N2E2_1806 at 2137.073 kb on + strand, within Pf6N2E2_1806 at 2137.188 kb on + strand at 2137.188 kb on + strand at 2137.260 kb on + strand at 2137.260 kb on + strand at 2137.260 kb on + strand at 2137.260 kb on + strand at 2137.261 kb on - strand at 2137.261 kb on - strand at 2137.261 kb on - strand at 2137.261 kb on - strand at 2137.262 kb on + strand at 2137.262 kb on + strand at 2137.263 kb on - strand at 2137.263 kb on - strand at 2137.263 kb on - strand at 2137.267 kb on - strand at 2137.267 kb on - strand at 2137.267 kb on - strand at 2137.554 kb on + strand, within Pf6N2E2_1807 at 2137.956 kb on - strand, within Pf6N2E2_1807 at 2138.172 kb on + strand, within Pf6N2E2_1807 at 2138.173 kb on - strand, within Pf6N2E2_1807 at 2138.173 kb on - strand, within Pf6N2E2_1807 at 2138.179 kb on + strand, within Pf6N2E2_1807 at 2138.886 kb on + strand, within Pf6N2E2_1807 at 2138.977 kb on + strand, within Pf6N2E2_1807 at 2139.018 kb on + strand, within Pf6N2E2_1807 at 2139.018 kb on + strand, within Pf6N2E2_1807 at 2139.019 kb on - strand, within Pf6N2E2_1807 at 2139.079 kb on - strand, within Pf6N2E2_1807 at 2139.079 kb on - strand, within Pf6N2E2_1807 at 2139.101 kb on + strand, within Pf6N2E2_1807 at 2139.102 kb on - strand, within Pf6N2E2_1807 at 2139.102 kb on - strand, within Pf6N2E2_1807 at 2139.158 kb on - strand, within Pf6N2E2_1807 at 2139.312 kb on - strand, within Pf6N2E2_1807 at 2139.331 kb on + strand, within Pf6N2E2_1807 at 2139.405 kb on + strand at 2139.406 kb on - strand at 2139.406 kb on - strand at 2139.554 kb on - strand at 2139.688 kb on - strand at 2139.816 kb on - strand, within Pf6N2E2_1808 at 2139.839 kb on + strand, within Pf6N2E2_1808 at 2139.840 kb on - strand, within Pf6N2E2_1808 at 2139.840 kb on - strand, within Pf6N2E2_1808 at 2139.841 kb on + strand, within Pf6N2E2_1808 at 2139.856 kb on + strand, within Pf6N2E2_1808 at 2139.856 kb on + strand, within Pf6N2E2_1808 at 2139.857 kb on - strand, within Pf6N2E2_1808 at 2139.857 kb on - strand, within Pf6N2E2_1808 at 2139.861 kb on - strand, within Pf6N2E2_1808 at 2140.175 kb on - strand, within Pf6N2E2_1808 at 2140.210 kb on + strand, within Pf6N2E2_1808
Per-strain Table
Position Strand Gene LocusTag Fraction Hans 100x vitamins remove 2,136,384 + -0.3 2,136,385 - -1.1 2,136,758 + +0.1 2,136,921 - Pf6N2E2_1806 0.37 -0.7 2,137,073 + Pf6N2E2_1806 0.67 +0.6 2,137,188 + -0.5 2,137,188 + +0.0 2,137,260 + +0.5 2,137,260 + -0.6 2,137,260 + +0.7 2,137,260 + -0.9 2,137,261 - +0.5 2,137,261 - -0.1 2,137,261 - +0.8 2,137,261 - +0.2 2,137,262 + +0.1 2,137,262 + -0.8 2,137,263 - +0.5 2,137,263 - -0.3 2,137,263 - -0.4 2,137,267 - +0.9 2,137,267 - -0.4 2,137,267 - +0.4 2,137,554 + Pf6N2E2_1807 0.11 -0.2 2,137,956 - Pf6N2E2_1807 0.29 +0.8 2,138,172 + Pf6N2E2_1807 0.39 -0.0 2,138,173 - Pf6N2E2_1807 0.39 -0.7 2,138,173 - Pf6N2E2_1807 0.39 -0.3 2,138,179 + Pf6N2E2_1807 0.39 -0.6 2,138,886 + Pf6N2E2_1807 0.70 -0.6 2,138,977 + Pf6N2E2_1807 0.74 -0.7 2,139,018 + Pf6N2E2_1807 0.76 -0.8 2,139,018 + Pf6N2E2_1807 0.76 -0.6 2,139,019 - Pf6N2E2_1807 0.76 -0.7 2,139,079 - Pf6N2E2_1807 0.78 -0.1 2,139,079 - Pf6N2E2_1807 0.78 -0.4 2,139,101 + Pf6N2E2_1807 0.79 -1.0 2,139,102 - Pf6N2E2_1807 0.79 -0.0 2,139,102 - Pf6N2E2_1807 0.79 -1.0 2,139,158 - Pf6N2E2_1807 0.82 -0.8 2,139,312 - Pf6N2E2_1807 0.89 +0.1 2,139,331 + Pf6N2E2_1807 0.89 -1.3 2,139,405 + +0.4 2,139,406 - -0.8 2,139,406 - -0.8 2,139,554 - +0.4 2,139,688 - -0.3 2,139,816 - Pf6N2E2_1808 0.21 -0.8 2,139,839 + Pf6N2E2_1808 0.23 -0.2 2,139,840 - Pf6N2E2_1808 0.24 +0.8 2,139,840 - Pf6N2E2_1808 0.24 -0.6 2,139,841 + Pf6N2E2_1808 0.24 -1.5 2,139,856 + Pf6N2E2_1808 0.25 -1.6 2,139,856 + Pf6N2E2_1808 0.25 -1.9 2,139,857 - Pf6N2E2_1808 0.25 -0.8 2,139,857 - Pf6N2E2_1808 0.25 -0.5 2,139,861 - Pf6N2E2_1808 0.26 +1.1 2,140,175 - Pf6N2E2_1808 0.60 +0.2 2,140,210 + Pf6N2E2_1808 0.64 -0.1
Or see this region's nucleotide sequence