Experiment: Hans 100x vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1695 and Pf6N2E2_1696 are separated by 265 nucleotides Pf6N2E2_1696 and Pf6N2E2_1697 are separated by 116 nucleotides
Pf6N2E2_1695 - Acyl-CoA dehydrogenase; probable dibenzothiophene desulfurization enzyme, at 2,003,117 to 2,004,304
_1695
Pf6N2E2_1696 - Alkanesulfonates-binding protein, at 2,004,570 to 2,005,418
_1696
Pf6N2E2_1697 - Alkanesulfonate monooxygenase (EC 1.14.14.5), at 2,005,535 to 2,006,665
_1697
Position (kb)
2004
2005
2006 Strain fitness (log2 ratio)
-2
-1
0
1 at 2003.687 kb on - strand, within Pf6N2E2_1695 at 2003.687 kb on - strand, within Pf6N2E2_1695 at 2003.740 kb on - strand, within Pf6N2E2_1695 at 2003.755 kb on - strand, within Pf6N2E2_1695 at 2003.876 kb on + strand, within Pf6N2E2_1695 at 2003.939 kb on + strand, within Pf6N2E2_1695 at 2003.939 kb on + strand, within Pf6N2E2_1695 at 2003.940 kb on - strand, within Pf6N2E2_1695 at 2003.940 kb on - strand, within Pf6N2E2_1695 at 2003.940 kb on - strand, within Pf6N2E2_1695 at 2004.296 kb on + strand at 2004.296 kb on + strand at 2004.296 kb on + strand at 2004.297 kb on - strand at 2004.297 kb on - strand at 2004.297 kb on - strand at 2004.297 kb on - strand at 2004.302 kb on + strand at 2004.392 kb on - strand at 2004.392 kb on - strand at 2004.849 kb on + strand, within Pf6N2E2_1696 at 2004.850 kb on - strand, within Pf6N2E2_1696 at 2004.942 kb on + strand, within Pf6N2E2_1696 at 2005.008 kb on + strand, within Pf6N2E2_1696 at 2005.009 kb on - strand, within Pf6N2E2_1696 at 2005.416 kb on + strand at 2005.426 kb on - strand at 2005.584 kb on - strand at 2005.626 kb on - strand at 2005.626 kb on - strand at 2006.057 kb on + strand, within Pf6N2E2_1697 at 2006.114 kb on + strand, within Pf6N2E2_1697 at 2006.114 kb on + strand, within Pf6N2E2_1697 at 2006.115 kb on - strand, within Pf6N2E2_1697 at 2006.115 kb on - strand, within Pf6N2E2_1697 at 2006.115 kb on - strand, within Pf6N2E2_1697 at 2006.319 kb on - strand, within Pf6N2E2_1697
Per-strain Table
Position Strand Gene LocusTag Fraction Hans 100x vitamins remove 2,003,687 - Pf6N2E2_1695 0.48 -0.7 2,003,687 - Pf6N2E2_1695 0.48 +0.5 2,003,740 - Pf6N2E2_1695 0.52 -0.5 2,003,755 - Pf6N2E2_1695 0.54 -0.2 2,003,876 + Pf6N2E2_1695 0.64 -0.2 2,003,939 + Pf6N2E2_1695 0.69 -0.3 2,003,939 + Pf6N2E2_1695 0.69 -0.5 2,003,940 - Pf6N2E2_1695 0.69 -0.6 2,003,940 - Pf6N2E2_1695 0.69 +0.7 2,003,940 - Pf6N2E2_1695 0.69 +1.4 2,004,296 + +0.5 2,004,296 + +0.1 2,004,296 + -0.1 2,004,297 - -0.9 2,004,297 - +0.1 2,004,297 - -0.6 2,004,297 - -0.1 2,004,302 + -0.5 2,004,392 - -0.6 2,004,392 - -0.3 2,004,849 + Pf6N2E2_1696 0.33 -1.1 2,004,850 - Pf6N2E2_1696 0.33 -0.1 2,004,942 + Pf6N2E2_1696 0.44 -1.1 2,005,008 + Pf6N2E2_1696 0.52 -1.9 2,005,009 - Pf6N2E2_1696 0.52 -0.4 2,005,416 + -0.2 2,005,426 - -1.2 2,005,584 - +0.5 2,005,626 - -1.8 2,005,626 - -1.1 2,006,057 + Pf6N2E2_1697 0.46 -0.1 2,006,114 + Pf6N2E2_1697 0.51 -0.4 2,006,114 + Pf6N2E2_1697 0.51 -0.3 2,006,115 - Pf6N2E2_1697 0.51 +0.3 2,006,115 - Pf6N2E2_1697 0.51 +0.8 2,006,115 - Pf6N2E2_1697 0.51 +1.1 2,006,319 - Pf6N2E2_1697 0.69 -1.5
Or see this region's nucleotide sequence