Experiment: Hans 100x vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1532 and Pf6N2E2_1533 are separated by 28 nucleotides Pf6N2E2_1533 and Pf6N2E2_1534 are separated by 19 nucleotides Pf6N2E2_1534 and Pf6N2E2_1535 overlap by 4 nucleotides Pf6N2E2_1535 and Pf6N2E2_1536 are separated by 13 nucleotides
Pf6N2E2_1532 - FIG003847: Oxidoreductase (flavoprotein), at 1,792,255 to 1,793,925
_1532
Pf6N2E2_1533 - membrane protein, putative, at 1,793,954 to 1,794,757
_1533
Pf6N2E2_1534 - Probable transmembrane protein, at 1,794,777 to 1,795,235
_1534
Pf6N2E2_1535 - GENE II AND X PROTEINS, at 1,795,232 to 1,795,657
_1535
Pf6N2E2_1536 - Permeases of the major facilitator superfamily, at 1,795,671 to 1,795,859
_1536
Position (kb)
1793
1794
1795 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1793.080 kb on + strand, within Pf6N2E2_1532 at 1793.080 kb on + strand, within Pf6N2E2_1532 at 1793.080 kb on + strand, within Pf6N2E2_1532 at 1793.081 kb on - strand, within Pf6N2E2_1532 at 1793.081 kb on - strand, within Pf6N2E2_1532 at 1793.081 kb on - strand, within Pf6N2E2_1532 at 1793.081 kb on - strand, within Pf6N2E2_1532 at 1793.086 kb on + strand, within Pf6N2E2_1532 at 1793.087 kb on - strand, within Pf6N2E2_1532 at 1793.101 kb on + strand, within Pf6N2E2_1532 at 1793.102 kb on - strand, within Pf6N2E2_1532 at 1793.200 kb on + strand, within Pf6N2E2_1532 at 1793.200 kb on + strand, within Pf6N2E2_1532 at 1793.200 kb on + strand, within Pf6N2E2_1532 at 1793.201 kb on - strand, within Pf6N2E2_1532 at 1793.201 kb on - strand, within Pf6N2E2_1532 at 1793.201 kb on - strand, within Pf6N2E2_1532 at 1793.201 kb on - strand, within Pf6N2E2_1532 at 1793.201 kb on - strand, within Pf6N2E2_1532 at 1793.296 kb on + strand, within Pf6N2E2_1532 at 1793.297 kb on - strand, within Pf6N2E2_1532 at 1793.329 kb on + strand, within Pf6N2E2_1532 at 1793.330 kb on - strand, within Pf6N2E2_1532 at 1793.355 kb on + strand, within Pf6N2E2_1532 at 1793.356 kb on - strand, within Pf6N2E2_1532 at 1793.421 kb on + strand, within Pf6N2E2_1532 at 1793.495 kb on + strand, within Pf6N2E2_1532 at 1793.554 kb on - strand, within Pf6N2E2_1532 at 1793.554 kb on - strand, within Pf6N2E2_1532 at 1793.652 kb on + strand, within Pf6N2E2_1532 at 1793.695 kb on + strand, within Pf6N2E2_1532 at 1793.761 kb on + strand at 1793.808 kb on + strand at 1793.838 kb on + strand at 1793.838 kb on + strand at 1793.838 kb on + strand at 1793.838 kb on + strand at 1793.838 kb on + strand at 1793.839 kb on - strand at 1793.839 kb on - strand at 1793.839 kb on - strand at 1793.863 kb on + strand at 1793.921 kb on + strand at 1793.921 kb on + strand at 1793.921 kb on + strand at 1793.922 kb on - strand at 1793.922 kb on - strand at 1794.024 kb on + strand at 1794.155 kb on + strand, within Pf6N2E2_1533 at 1794.272 kb on + strand, within Pf6N2E2_1533 at 1794.272 kb on + strand, within Pf6N2E2_1533 at 1794.272 kb on + strand, within Pf6N2E2_1533 at 1794.272 kb on + strand, within Pf6N2E2_1533 at 1794.272 kb on + strand, within Pf6N2E2_1533 at 1794.273 kb on - strand at 1794.273 kb on - strand, within Pf6N2E2_1533 at 1794.273 kb on - strand, within Pf6N2E2_1533 at 1794.273 kb on - strand, within Pf6N2E2_1533 at 1794.615 kb on + strand, within Pf6N2E2_1533 at 1794.686 kb on + strand at 1794.687 kb on - strand at 1794.741 kb on + strand at 1794.741 kb on + strand at 1794.772 kb on + strand at 1794.772 kb on + strand at 1794.825 kb on - strand, within Pf6N2E2_1534 at 1794.825 kb on - strand, within Pf6N2E2_1534 at 1795.204 kb on + strand at 1795.204 kb on + strand at 1795.204 kb on + strand at 1795.205 kb on - strand at 1795.709 kb on + strand, within Pf6N2E2_1536 at 1795.709 kb on + strand, within Pf6N2E2_1536 at 1795.710 kb on - strand, within Pf6N2E2_1536 at 1795.710 kb on - strand, within Pf6N2E2_1536 at 1795.730 kb on + strand, within Pf6N2E2_1536 at 1795.730 kb on + strand, within Pf6N2E2_1536 at 1795.732 kb on + strand, within Pf6N2E2_1536 at 1795.732 kb on + strand, within Pf6N2E2_1536 at 1795.733 kb on - strand, within Pf6N2E2_1536
Per-strain Table
Position Strand Gene LocusTag Fraction Hans 100x vitamins remove 1,793,080 + Pf6N2E2_1532 0.49 -1.7 1,793,080 + Pf6N2E2_1532 0.49 +0.1 1,793,080 + Pf6N2E2_1532 0.49 +0.7 1,793,081 - Pf6N2E2_1532 0.49 -0.1 1,793,081 - Pf6N2E2_1532 0.49 +1.5 1,793,081 - Pf6N2E2_1532 0.49 +1.1 1,793,081 - Pf6N2E2_1532 0.49 +0.5 1,793,086 + Pf6N2E2_1532 0.50 -0.0 1,793,087 - Pf6N2E2_1532 0.50 +0.9 1,793,101 + Pf6N2E2_1532 0.51 -0.8 1,793,102 - Pf6N2E2_1532 0.51 -0.3 1,793,200 + Pf6N2E2_1532 0.57 +0.7 1,793,200 + Pf6N2E2_1532 0.57 +0.1 1,793,200 + Pf6N2E2_1532 0.57 -1.3 1,793,201 - Pf6N2E2_1532 0.57 +1.5 1,793,201 - Pf6N2E2_1532 0.57 +1.1 1,793,201 - Pf6N2E2_1532 0.57 -0.3 1,793,201 - Pf6N2E2_1532 0.57 +0.5 1,793,201 - Pf6N2E2_1532 0.57 -0.8 1,793,296 + Pf6N2E2_1532 0.62 +1.1 1,793,297 - Pf6N2E2_1532 0.62 +0.8 1,793,329 + Pf6N2E2_1532 0.64 +0.9 1,793,330 - Pf6N2E2_1532 0.64 +1.0 1,793,355 + Pf6N2E2_1532 0.66 -0.2 1,793,356 - Pf6N2E2_1532 0.66 +0.8 1,793,421 + Pf6N2E2_1532 0.70 +0.0 1,793,495 + Pf6N2E2_1532 0.74 -0.8 1,793,554 - Pf6N2E2_1532 0.78 +1.0 1,793,554 - Pf6N2E2_1532 0.78 +0.3 1,793,652 + Pf6N2E2_1532 0.84 -1.9 1,793,695 + Pf6N2E2_1532 0.86 -0.5 1,793,761 + -0.8 1,793,808 + -2.1 1,793,838 + +1.1 1,793,838 + +0.3 1,793,838 + +0.5 1,793,838 + +0.1 1,793,838 + -1.5 1,793,839 - +0.7 1,793,839 - -0.8 1,793,839 - -0.8 1,793,863 + -0.4 1,793,921 + -0.2 1,793,921 + +0.3 1,793,921 + +0.1 1,793,922 - -0.9 1,793,922 - +1.5 1,794,024 + +1.0 1,794,155 + Pf6N2E2_1533 0.25 -0.3 1,794,272 + Pf6N2E2_1533 0.40 +0.5 1,794,272 + Pf6N2E2_1533 0.40 -0.6 1,794,272 + Pf6N2E2_1533 0.40 -1.8 1,794,272 + Pf6N2E2_1533 0.40 -0.6 1,794,272 + Pf6N2E2_1533 0.40 +1.8 1,794,273 - +1.5 1,794,273 - Pf6N2E2_1533 0.40 -1.3 1,794,273 - Pf6N2E2_1533 0.40 -0.8 1,794,273 - Pf6N2E2_1533 0.40 -0.5 1,794,615 + Pf6N2E2_1533 0.82 -0.6 1,794,686 + -0.9 1,794,687 - +0.5 1,794,741 + -1.3 1,794,741 + -0.1 1,794,772 + -0.7 1,794,772 + +1.5 1,794,825 - Pf6N2E2_1534 0.10 +1.0 1,794,825 - Pf6N2E2_1534 0.10 -1.1 1,795,204 + -0.7 1,795,204 + -0.2 1,795,204 + -0.1 1,795,205 - -0.5 1,795,709 + Pf6N2E2_1536 0.20 +1.4 1,795,709 + Pf6N2E2_1536 0.20 -0.9 1,795,710 - Pf6N2E2_1536 0.21 +1.5 1,795,710 - Pf6N2E2_1536 0.21 -0.8 1,795,730 + Pf6N2E2_1536 0.31 -1.1 1,795,730 + Pf6N2E2_1536 0.31 +0.4 1,795,732 + Pf6N2E2_1536 0.32 -0.4 1,795,732 + Pf6N2E2_1536 0.32 +0.5 1,795,733 - Pf6N2E2_1536 0.33 +0.3
Or see this region's nucleotide sequence