Experiment: Hans 100x vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1505 and Pf6N2E2_1506 are separated by 215 nucleotides Pf6N2E2_1506 and Pf6N2E2_1507 are separated by 89 nucleotides
Pf6N2E2_1505 - Transporter, MFS superfamily, at 1,759,095 to 1,760,291
_1505
Pf6N2E2_1506 - Putative alkanesulfonate metabolism utilization regulator, at 1,760,507 to 1,761,238
_1506
Pf6N2E2_1507 - Sensor protein basS/pmrB (EC 2.7.3.-), at 1,761,328 to 1,762,686
_1507
Position (kb)
1760
1761
1762 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1759.588 kb on + strand, within Pf6N2E2_1505 at 1759.901 kb on - strand, within Pf6N2E2_1505 at 1759.957 kb on - strand, within Pf6N2E2_1505 at 1759.957 kb on - strand, within Pf6N2E2_1505 at 1760.158 kb on + strand, within Pf6N2E2_1505 at 1760.295 kb on + strand at 1760.295 kb on + strand at 1760.296 kb on - strand at 1760.296 kb on - strand at 1760.296 kb on - strand at 1760.296 kb on - strand at 1760.650 kb on - strand, within Pf6N2E2_1506 at 1760.721 kb on + strand, within Pf6N2E2_1506 at 1760.722 kb on - strand, within Pf6N2E2_1506 at 1760.849 kb on - strand, within Pf6N2E2_1506 at 1761.105 kb on + strand, within Pf6N2E2_1506 at 1761.106 kb on - strand, within Pf6N2E2_1506 at 1761.201 kb on + strand at 1761.202 kb on - strand at 1761.246 kb on - strand at 1761.269 kb on + strand at 1761.272 kb on - strand at 1761.289 kb on + strand at 1761.289 kb on + strand at 1761.418 kb on + strand at 1761.419 kb on - strand at 1761.626 kb on + strand, within Pf6N2E2_1507 at 1761.627 kb on - strand, within Pf6N2E2_1507 at 1761.627 kb on - strand, within Pf6N2E2_1507 at 1761.693 kb on - strand, within Pf6N2E2_1507 at 1761.926 kb on + strand, within Pf6N2E2_1507 at 1761.966 kb on + strand, within Pf6N2E2_1507 at 1761.966 kb on + strand, within Pf6N2E2_1507 at 1761.966 kb on + strand, within Pf6N2E2_1507 at 1761.967 kb on - strand, within Pf6N2E2_1507 at 1761.967 kb on - strand, within Pf6N2E2_1507 at 1761.967 kb on - strand, within Pf6N2E2_1507 at 1762.139 kb on + strand, within Pf6N2E2_1507 at 1762.230 kb on + strand, within Pf6N2E2_1507 at 1762.231 kb on - strand, within Pf6N2E2_1507 at 1762.231 kb on - strand, within Pf6N2E2_1507
Per-strain Table
Position Strand Gene LocusTag Fraction Hans 100x vitamins remove 1,759,588 + Pf6N2E2_1505 0.41 +0.3 1,759,901 - Pf6N2E2_1505 0.67 +1.2 1,759,957 - Pf6N2E2_1505 0.72 -0.8 1,759,957 - Pf6N2E2_1505 0.72 +1.2 1,760,158 + Pf6N2E2_1505 0.89 +0.4 1,760,295 + +0.5 1,760,295 + +0.5 1,760,296 - +0.5 1,760,296 - +1.0 1,760,296 - -1.0 1,760,296 - -0.7 1,760,650 - Pf6N2E2_1506 0.20 -0.7 1,760,721 + Pf6N2E2_1506 0.29 -0.3 1,760,722 - Pf6N2E2_1506 0.29 -2.0 1,760,849 - Pf6N2E2_1506 0.47 -0.3 1,761,105 + Pf6N2E2_1506 0.82 +1.5 1,761,106 - Pf6N2E2_1506 0.82 -1.1 1,761,201 + +0.5 1,761,202 - +1.2 1,761,246 - +1.5 1,761,269 + -0.7 1,761,272 - +1.5 1,761,289 + -0.6 1,761,289 + -0.1 1,761,418 + +0.9 1,761,419 - +0.5 1,761,626 + Pf6N2E2_1507 0.22 +0.2 1,761,627 - Pf6N2E2_1507 0.22 +0.6 1,761,627 - Pf6N2E2_1507 0.22 +1.0 1,761,693 - Pf6N2E2_1507 0.27 -1.1 1,761,926 + Pf6N2E2_1507 0.44 +0.9 1,761,966 + Pf6N2E2_1507 0.47 +0.2 1,761,966 + Pf6N2E2_1507 0.47 +0.7 1,761,966 + Pf6N2E2_1507 0.47 +3.1 1,761,967 - Pf6N2E2_1507 0.47 -0.5 1,761,967 - Pf6N2E2_1507 0.47 +0.2 1,761,967 - Pf6N2E2_1507 0.47 -0.1 1,762,139 + Pf6N2E2_1507 0.60 +0.2 1,762,230 + Pf6N2E2_1507 0.66 +0.1 1,762,231 - Pf6N2E2_1507 0.66 -0.4 1,762,231 - Pf6N2E2_1507 0.66 -0.0
Or see this region's nucleotide sequence