Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1472

Experiment: Hans 100x vitamins

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1470 and Pf6N2E2_1471 are separated by 100 nucleotidesPf6N2E2_1471 and Pf6N2E2_1472 are separated by 18 nucleotidesPf6N2E2_1472 and Pf6N2E2_1473 are separated by 280 nucleotidesPf6N2E2_1473 and Pf6N2E2_1474 are separated by 23 nucleotides Pf6N2E2_1470 - Transcriptional regulator, LysR family, at 1,722,576 to 1,722,734 _1470 Pf6N2E2_1471 - Dienelactone hydrolase and related enzymes, at 1,722,835 to 1,723,065 _1471 Pf6N2E2_1472 - Cytochrome b561, at 1,723,084 to 1,723,635 _1472 Pf6N2E2_1473 - hypothetical protein, at 1,723,916 to 1,724,077 _1473 Pf6N2E2_1474 - SAM-dependent methyltransferases, at 1,724,101 to 1,724,697 _1474 Position (kb) 1723 1724Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1722.186 kb on + strandat 1722.358 kb on + strandat 1722.476 kb on - strandat 1722.503 kb on - strandat 1722.539 kb on + strandat 1722.539 kb on + strandat 1722.540 kb on - strandat 1722.619 kb on + strand, within Pf6N2E2_1470at 1722.619 kb on + strand, within Pf6N2E2_1470at 1722.654 kb on - strand, within Pf6N2E2_1470at 1722.709 kb on + strand, within Pf6N2E2_1470at 1722.709 kb on + strand, within Pf6N2E2_1470at 1722.710 kb on - strand, within Pf6N2E2_1470at 1722.887 kb on + strand, within Pf6N2E2_1471at 1722.888 kb on - strand, within Pf6N2E2_1471at 1722.938 kb on - strand, within Pf6N2E2_1471at 1722.938 kb on - strand, within Pf6N2E2_1471at 1722.938 kb on - strand, within Pf6N2E2_1471at 1722.938 kb on - strand, within Pf6N2E2_1471at 1723.000 kb on + strand, within Pf6N2E2_1471at 1723.001 kb on - strand, within Pf6N2E2_1471at 1723.123 kb on - strandat 1723.140 kb on - strand, within Pf6N2E2_1472at 1723.201 kb on + strand, within Pf6N2E2_1472at 1723.202 kb on - strand, within Pf6N2E2_1472at 1723.214 kb on + strandat 1723.215 kb on - strand, within Pf6N2E2_1472at 1723.215 kb on - strand, within Pf6N2E2_1472at 1723.346 kb on + strand, within Pf6N2E2_1472at 1723.550 kb on + strand, within Pf6N2E2_1472at 1723.550 kb on + strand, within Pf6N2E2_1472at 1723.550 kb on + strand, within Pf6N2E2_1472at 1723.550 kb on + strand, within Pf6N2E2_1472at 1723.551 kb on - strand, within Pf6N2E2_1472at 1723.768 kb on - strandat 1723.806 kb on - strandat 1723.881 kb on - strandat 1724.003 kb on + strand, within Pf6N2E2_1473at 1724.003 kb on + strand, within Pf6N2E2_1473at 1724.113 kb on - strandat 1724.328 kb on - strand, within Pf6N2E2_1474at 1724.328 kb on - strand, within Pf6N2E2_1474at 1724.328 kb on - strand, within Pf6N2E2_1474at 1724.328 kb on - strand, within Pf6N2E2_1474at 1724.334 kb on - strand, within Pf6N2E2_1474at 1724.334 kb on - strand, within Pf6N2E2_1474at 1724.372 kb on - strand, within Pf6N2E2_1474at 1724.395 kb on + strand, within Pf6N2E2_1474at 1724.396 kb on - strand, within Pf6N2E2_1474at 1724.396 kb on - strand, within Pf6N2E2_1474at 1724.434 kb on + strand, within Pf6N2E2_1474at 1724.459 kb on + strand, within Pf6N2E2_1474at 1724.459 kb on + strand, within Pf6N2E2_1474at 1724.460 kb on - strand, within Pf6N2E2_1474at 1724.460 kb on - strand, within Pf6N2E2_1474at 1724.460 kb on - strand, within Pf6N2E2_1474at 1724.460 kb on - strand, within Pf6N2E2_1474at 1724.560 kb on - strand, within Pf6N2E2_1474at 1724.560 kb on - strand, within Pf6N2E2_1474

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Per-strain Table

Position Strand Gene LocusTag Fraction Hans 100x vitamins
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1,722,186 + -0.4
1,722,358 + +0.4
1,722,476 - +0.8
1,722,503 - -0.8
1,722,539 + -1.1
1,722,539 + +0.6
1,722,540 - +1.4
1,722,619 + Pf6N2E2_1470 0.27 +0.5
1,722,619 + Pf6N2E2_1470 0.27 -0.1
1,722,654 - Pf6N2E2_1470 0.49 -0.1
1,722,709 + Pf6N2E2_1470 0.84 +1.1
1,722,709 + Pf6N2E2_1470 0.84 -1.0
1,722,710 - Pf6N2E2_1470 0.84 -1.2
1,722,887 + Pf6N2E2_1471 0.23 +0.6
1,722,888 - Pf6N2E2_1471 0.23 +1.2
1,722,938 - Pf6N2E2_1471 0.45 +1.6
1,722,938 - Pf6N2E2_1471 0.45 -0.1
1,722,938 - Pf6N2E2_1471 0.45 +0.6
1,722,938 - Pf6N2E2_1471 0.45 +0.1
1,723,000 + Pf6N2E2_1471 0.71 -0.1
1,723,001 - Pf6N2E2_1471 0.72 -0.1
1,723,123 - +1.4
1,723,140 - Pf6N2E2_1472 0.10 -0.4
1,723,201 + Pf6N2E2_1472 0.21 -0.6
1,723,202 - Pf6N2E2_1472 0.21 -0.3
1,723,214 + +0.4
1,723,215 - Pf6N2E2_1472 0.24 -1.4
1,723,215 - Pf6N2E2_1472 0.24 -0.5
1,723,346 + Pf6N2E2_1472 0.47 +0.4
1,723,550 + Pf6N2E2_1472 0.84 +0.1
1,723,550 + Pf6N2E2_1472 0.84 -2.5
1,723,550 + Pf6N2E2_1472 0.84 -1.6
1,723,550 + Pf6N2E2_1472 0.84 -0.9
1,723,551 - Pf6N2E2_1472 0.85 +0.4
1,723,768 - +0.0
1,723,806 - -0.5
1,723,881 - +0.1
1,724,003 + Pf6N2E2_1473 0.54 +0.0
1,724,003 + Pf6N2E2_1473 0.54 -0.6
1,724,113 - -0.7
1,724,328 - Pf6N2E2_1474 0.38 -1.3
1,724,328 - Pf6N2E2_1474 0.38 +0.1
1,724,328 - Pf6N2E2_1474 0.38 -2.4
1,724,328 - Pf6N2E2_1474 0.38 +1.0
1,724,334 - Pf6N2E2_1474 0.39 -0.1
1,724,334 - Pf6N2E2_1474 0.39 +0.1
1,724,372 - Pf6N2E2_1474 0.45 -2.3
1,724,395 + Pf6N2E2_1474 0.49 +0.2
1,724,396 - Pf6N2E2_1474 0.49 +3.4
1,724,396 - Pf6N2E2_1474 0.49 -0.5
1,724,434 + Pf6N2E2_1474 0.56 +0.1
1,724,459 + Pf6N2E2_1474 0.60 +1.4
1,724,459 + Pf6N2E2_1474 0.60 -0.6
1,724,460 - Pf6N2E2_1474 0.60 -0.6
1,724,460 - Pf6N2E2_1474 0.60 +1.0
1,724,460 - Pf6N2E2_1474 0.60 +0.3
1,724,460 - Pf6N2E2_1474 0.60 -0.8
1,724,560 - Pf6N2E2_1474 0.77 +1.4
1,724,560 - Pf6N2E2_1474 0.77 -0.3

Or see this region's nucleotide sequence