Experiment: Hans 100x vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1470 and Pf6N2E2_1471 are separated by 100 nucleotides Pf6N2E2_1471 and Pf6N2E2_1472 are separated by 18 nucleotides Pf6N2E2_1472 and Pf6N2E2_1473 are separated by 280 nucleotides Pf6N2E2_1473 and Pf6N2E2_1474 are separated by 23 nucleotides
Pf6N2E2_1470 - Transcriptional regulator, LysR family, at 1,722,576 to 1,722,734
_1470
Pf6N2E2_1471 - Dienelactone hydrolase and related enzymes, at 1,722,835 to 1,723,065
_1471
Pf6N2E2_1472 - Cytochrome b561, at 1,723,084 to 1,723,635
_1472
Pf6N2E2_1473 - hypothetical protein, at 1,723,916 to 1,724,077
_1473
Pf6N2E2_1474 - SAM-dependent methyltransferases, at 1,724,101 to 1,724,697
_1474
Position (kb)
1723
1724 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1722.186 kb on + strand at 1722.358 kb on + strand at 1722.476 kb on - strand at 1722.503 kb on - strand at 1722.539 kb on + strand at 1722.539 kb on + strand at 1722.540 kb on - strand at 1722.619 kb on + strand, within Pf6N2E2_1470 at 1722.619 kb on + strand, within Pf6N2E2_1470 at 1722.654 kb on - strand, within Pf6N2E2_1470 at 1722.709 kb on + strand, within Pf6N2E2_1470 at 1722.709 kb on + strand, within Pf6N2E2_1470 at 1722.710 kb on - strand, within Pf6N2E2_1470 at 1722.887 kb on + strand, within Pf6N2E2_1471 at 1722.888 kb on - strand, within Pf6N2E2_1471 at 1722.938 kb on - strand, within Pf6N2E2_1471 at 1722.938 kb on - strand, within Pf6N2E2_1471 at 1722.938 kb on - strand, within Pf6N2E2_1471 at 1722.938 kb on - strand, within Pf6N2E2_1471 at 1723.000 kb on + strand, within Pf6N2E2_1471 at 1723.001 kb on - strand, within Pf6N2E2_1471 at 1723.123 kb on - strand at 1723.140 kb on - strand, within Pf6N2E2_1472 at 1723.201 kb on + strand, within Pf6N2E2_1472 at 1723.202 kb on - strand, within Pf6N2E2_1472 at 1723.214 kb on + strand at 1723.215 kb on - strand, within Pf6N2E2_1472 at 1723.215 kb on - strand, within Pf6N2E2_1472 at 1723.346 kb on + strand, within Pf6N2E2_1472 at 1723.550 kb on + strand, within Pf6N2E2_1472 at 1723.550 kb on + strand, within Pf6N2E2_1472 at 1723.550 kb on + strand, within Pf6N2E2_1472 at 1723.550 kb on + strand, within Pf6N2E2_1472 at 1723.551 kb on - strand, within Pf6N2E2_1472 at 1723.768 kb on - strand at 1723.806 kb on - strand at 1723.881 kb on - strand at 1724.003 kb on + strand, within Pf6N2E2_1473 at 1724.003 kb on + strand, within Pf6N2E2_1473 at 1724.113 kb on - strand at 1724.328 kb on - strand, within Pf6N2E2_1474 at 1724.328 kb on - strand, within Pf6N2E2_1474 at 1724.328 kb on - strand, within Pf6N2E2_1474 at 1724.328 kb on - strand, within Pf6N2E2_1474 at 1724.334 kb on - strand, within Pf6N2E2_1474 at 1724.334 kb on - strand, within Pf6N2E2_1474 at 1724.372 kb on - strand, within Pf6N2E2_1474 at 1724.395 kb on + strand, within Pf6N2E2_1474 at 1724.396 kb on - strand, within Pf6N2E2_1474 at 1724.396 kb on - strand, within Pf6N2E2_1474 at 1724.434 kb on + strand, within Pf6N2E2_1474 at 1724.459 kb on + strand, within Pf6N2E2_1474 at 1724.459 kb on + strand, within Pf6N2E2_1474 at 1724.460 kb on - strand, within Pf6N2E2_1474 at 1724.460 kb on - strand, within Pf6N2E2_1474 at 1724.460 kb on - strand, within Pf6N2E2_1474 at 1724.460 kb on - strand, within Pf6N2E2_1474 at 1724.560 kb on - strand, within Pf6N2E2_1474 at 1724.560 kb on - strand, within Pf6N2E2_1474
Per-strain Table
Position Strand Gene LocusTag Fraction Hans 100x vitamins remove 1,722,186 + -0.4 1,722,358 + +0.4 1,722,476 - +0.8 1,722,503 - -0.8 1,722,539 + -1.1 1,722,539 + +0.6 1,722,540 - +1.4 1,722,619 + Pf6N2E2_1470 0.27 +0.5 1,722,619 + Pf6N2E2_1470 0.27 -0.1 1,722,654 - Pf6N2E2_1470 0.49 -0.1 1,722,709 + Pf6N2E2_1470 0.84 +1.1 1,722,709 + Pf6N2E2_1470 0.84 -1.0 1,722,710 - Pf6N2E2_1470 0.84 -1.2 1,722,887 + Pf6N2E2_1471 0.23 +0.6 1,722,888 - Pf6N2E2_1471 0.23 +1.2 1,722,938 - Pf6N2E2_1471 0.45 +1.6 1,722,938 - Pf6N2E2_1471 0.45 -0.1 1,722,938 - Pf6N2E2_1471 0.45 +0.6 1,722,938 - Pf6N2E2_1471 0.45 +0.1 1,723,000 + Pf6N2E2_1471 0.71 -0.1 1,723,001 - Pf6N2E2_1471 0.72 -0.1 1,723,123 - +1.4 1,723,140 - Pf6N2E2_1472 0.10 -0.4 1,723,201 + Pf6N2E2_1472 0.21 -0.6 1,723,202 - Pf6N2E2_1472 0.21 -0.3 1,723,214 + +0.4 1,723,215 - Pf6N2E2_1472 0.24 -1.4 1,723,215 - Pf6N2E2_1472 0.24 -0.5 1,723,346 + Pf6N2E2_1472 0.47 +0.4 1,723,550 + Pf6N2E2_1472 0.84 +0.1 1,723,550 + Pf6N2E2_1472 0.84 -2.5 1,723,550 + Pf6N2E2_1472 0.84 -1.6 1,723,550 + Pf6N2E2_1472 0.84 -0.9 1,723,551 - Pf6N2E2_1472 0.85 +0.4 1,723,768 - +0.0 1,723,806 - -0.5 1,723,881 - +0.1 1,724,003 + Pf6N2E2_1473 0.54 +0.0 1,724,003 + Pf6N2E2_1473 0.54 -0.6 1,724,113 - -0.7 1,724,328 - Pf6N2E2_1474 0.38 -1.3 1,724,328 - Pf6N2E2_1474 0.38 +0.1 1,724,328 - Pf6N2E2_1474 0.38 -2.4 1,724,328 - Pf6N2E2_1474 0.38 +1.0 1,724,334 - Pf6N2E2_1474 0.39 -0.1 1,724,334 - Pf6N2E2_1474 0.39 +0.1 1,724,372 - Pf6N2E2_1474 0.45 -2.3 1,724,395 + Pf6N2E2_1474 0.49 +0.2 1,724,396 - Pf6N2E2_1474 0.49 +3.4 1,724,396 - Pf6N2E2_1474 0.49 -0.5 1,724,434 + Pf6N2E2_1474 0.56 +0.1 1,724,459 + Pf6N2E2_1474 0.60 +1.4 1,724,459 + Pf6N2E2_1474 0.60 -0.6 1,724,460 - Pf6N2E2_1474 0.60 -0.6 1,724,460 - Pf6N2E2_1474 0.60 +1.0 1,724,460 - Pf6N2E2_1474 0.60 +0.3 1,724,460 - Pf6N2E2_1474 0.60 -0.8 1,724,560 - Pf6N2E2_1474 0.77 +1.4 1,724,560 - Pf6N2E2_1474 0.77 -0.3
Or see this region's nucleotide sequence