Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_136

Experiment: Hans 100x vitamins

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_135 and Pf6N2E2_136 are separated by 162 nucleotidesPf6N2E2_136 and Pf6N2E2_137 overlap by 1 nucleotides Pf6N2E2_135 - Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF, at 183,658 to 184,878 _135 Pf6N2E2_136 - Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE, at 185,041 to 186,099 _136 Pf6N2E2_137 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 186,099 to 187,634 _137 Position (kb) 185 186 187Strain fitness (log2 ratio) -2 -1 0 1 2at 184.106 kb on + strand, within Pf6N2E2_135at 184.239 kb on - strand, within Pf6N2E2_135at 184.607 kb on + strand, within Pf6N2E2_135at 184.608 kb on - strand, within Pf6N2E2_135at 184.705 kb on + strand, within Pf6N2E2_135at 184.706 kb on - strand, within Pf6N2E2_135at 184.706 kb on - strand, within Pf6N2E2_135at 184.706 kb on - strand, within Pf6N2E2_135at 184.721 kb on + strand, within Pf6N2E2_135at 184.721 kb on + strand, within Pf6N2E2_135at 184.722 kb on - strand, within Pf6N2E2_135at 184.722 kb on - strand, within Pf6N2E2_135at 184.722 kb on - strand, within Pf6N2E2_135at 184.722 kb on - strand, within Pf6N2E2_135at 184.722 kb on - strand, within Pf6N2E2_135at 184.767 kb on - strandat 184.878 kb on + strandat 184.878 kb on + strandat 184.878 kb on + strandat 184.878 kb on + strandat 184.878 kb on + strandat 184.878 kb on + strandat 184.878 kb on + strandat 184.878 kb on + strandat 184.878 kb on + strandat 184.879 kb on - strandat 184.879 kb on - strandat 184.879 kb on - strandat 184.879 kb on - strandat 184.879 kb on - strandat 184.879 kb on - strandat 184.879 kb on - strandat 184.879 kb on - strandat 184.879 kb on - strandat 184.882 kb on + strandat 184.882 kb on + strandat 184.882 kb on + strandat 184.883 kb on - strandat 184.883 kb on - strandat 184.883 kb on - strandat 184.893 kb on - strandat 185.042 kb on + strandat 185.043 kb on - strandat 185.054 kb on + strandat 185.055 kb on - strandat 185.114 kb on + strandat 185.376 kb on - strand, within Pf6N2E2_136at 185.557 kb on - strand, within Pf6N2E2_136at 185.557 kb on - strand, within Pf6N2E2_136at 185.928 kb on - strand, within Pf6N2E2_136at 186.545 kb on - strand, within Pf6N2E2_137at 186.547 kb on + strand, within Pf6N2E2_137at 186.547 kb on + strand, within Pf6N2E2_137at 186.547 kb on + strand, within Pf6N2E2_137at 186.547 kb on + strand, within Pf6N2E2_137at 186.548 kb on - strand, within Pf6N2E2_137at 186.548 kb on - strand, within Pf6N2E2_137at 186.548 kb on - strand, within Pf6N2E2_137at 187.085 kb on - strand, within Pf6N2E2_137

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Per-strain Table

Position Strand Gene LocusTag Fraction Hans 100x vitamins
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184,106 + Pf6N2E2_135 0.37 +0.4
184,239 - Pf6N2E2_135 0.48 +0.6
184,607 + Pf6N2E2_135 0.78 +0.6
184,608 - Pf6N2E2_135 0.78 -0.5
184,705 + Pf6N2E2_135 0.86 +0.9
184,706 - Pf6N2E2_135 0.86 -1.9
184,706 - Pf6N2E2_135 0.86 +0.6
184,706 - Pf6N2E2_135 0.86 -0.2
184,721 + Pf6N2E2_135 0.87 +0.1
184,721 + Pf6N2E2_135 0.87 -0.1
184,722 - Pf6N2E2_135 0.87 -0.1
184,722 - Pf6N2E2_135 0.87 -0.1
184,722 - Pf6N2E2_135 0.87 +1.2
184,722 - Pf6N2E2_135 0.87 +0.4
184,722 - Pf6N2E2_135 0.87 +1.1
184,767 - +0.3
184,878 + +0.7
184,878 + +0.6
184,878 + -0.3
184,878 + -1.0
184,878 + +0.0
184,878 + +1.0
184,878 + +0.6
184,878 + +0.4
184,878 + -0.4
184,879 - +0.1
184,879 - +0.3
184,879 - +0.9
184,879 - -0.2
184,879 - +0.2
184,879 - +0.3
184,879 - -0.0
184,879 - +0.4
184,879 - -0.3
184,882 + -0.9
184,882 + +0.5
184,882 + +0.6
184,883 - +0.2
184,883 - -0.2
184,883 - +1.8
184,893 - -0.3
185,042 + +0.4
185,043 - -0.5
185,054 + -1.5
185,055 - +0.6
185,114 + +0.2
185,376 - Pf6N2E2_136 0.32 +0.5
185,557 - Pf6N2E2_136 0.49 +0.7
185,557 - Pf6N2E2_136 0.49 -0.4
185,928 - Pf6N2E2_136 0.84 +1.2
186,545 - Pf6N2E2_137 0.29 +1.0
186,547 + Pf6N2E2_137 0.29 -0.1
186,547 + Pf6N2E2_137 0.29 +1.1
186,547 + Pf6N2E2_137 0.29 -0.1
186,547 + Pf6N2E2_137 0.29 +0.7
186,548 - Pf6N2E2_137 0.29 -0.5
186,548 - Pf6N2E2_137 0.29 +0.5
186,548 - Pf6N2E2_137 0.29 +0.4
187,085 - Pf6N2E2_137 0.64 +0.2

Or see this region's nucleotide sequence