Experiment: Hans 100x vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1328 and Pf6N2E2_1329 are separated by 208 nucleotides Pf6N2E2_1329 and Pf6N2E2_1330 are separated by 145 nucleotides
Pf6N2E2_1328 - Methyl-accepting chemotaxis protein, at 1,562,197 to 1,563,168
_1328
Pf6N2E2_1329 - Phenylacetaldehyde dehydrogenase (EC 1.2.1.39), at 1,563,377 to 1,564,864
_1329
Pf6N2E2_1330 - Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases, at 1,565,010 to 1,566,467
_1330
Position (kb)
1563
1564
1565 Strain fitness (log2 ratio)
-1
0
1
2 at 1562.405 kb on + strand, within Pf6N2E2_1328 at 1562.406 kb on - strand, within Pf6N2E2_1328 at 1562.406 kb on - strand, within Pf6N2E2_1328 at 1562.550 kb on - strand, within Pf6N2E2_1328 at 1562.550 kb on - strand, within Pf6N2E2_1328 at 1562.588 kb on + strand, within Pf6N2E2_1328 at 1562.589 kb on - strand, within Pf6N2E2_1328 at 1562.589 kb on - strand, within Pf6N2E2_1328 at 1562.860 kb on + strand, within Pf6N2E2_1328 at 1562.860 kb on + strand, within Pf6N2E2_1328 at 1562.861 kb on - strand, within Pf6N2E2_1328 at 1562.861 kb on - strand, within Pf6N2E2_1328 at 1563.293 kb on + strand at 1563.753 kb on - strand, within Pf6N2E2_1329 at 1563.828 kb on - strand, within Pf6N2E2_1329 at 1564.514 kb on + strand, within Pf6N2E2_1329 at 1564.514 kb on + strand, within Pf6N2E2_1329 at 1564.514 kb on + strand, within Pf6N2E2_1329 at 1564.514 kb on + strand, within Pf6N2E2_1329 at 1564.514 kb on + strand, within Pf6N2E2_1329 at 1564.514 kb on + strand, within Pf6N2E2_1329 at 1564.514 kb on + strand, within Pf6N2E2_1329 at 1564.515 kb on - strand, within Pf6N2E2_1329 at 1564.515 kb on - strand, within Pf6N2E2_1329 at 1564.515 kb on - strand, within Pf6N2E2_1329 at 1564.605 kb on - strand, within Pf6N2E2_1329 at 1564.829 kb on + strand at 1564.829 kb on + strand at 1564.829 kb on + strand at 1565.216 kb on - strand, within Pf6N2E2_1330 at 1565.327 kb on - strand, within Pf6N2E2_1330 at 1565.327 kb on - strand, within Pf6N2E2_1330 at 1565.327 kb on - strand, within Pf6N2E2_1330 at 1565.389 kb on + strand, within Pf6N2E2_1330 at 1565.497 kb on + strand, within Pf6N2E2_1330 at 1565.498 kb on - strand, within Pf6N2E2_1330 at 1565.646 kb on - strand, within Pf6N2E2_1330 at 1565.646 kb on - strand, within Pf6N2E2_1330 at 1565.717 kb on - strand, within Pf6N2E2_1330 at 1565.810 kb on + strand, within Pf6N2E2_1330
Per-strain Table
Position Strand Gene LocusTag Fraction Hans 100x vitamins remove 1,562,405 + Pf6N2E2_1328 0.21 +1.0 1,562,406 - Pf6N2E2_1328 0.22 +0.7 1,562,406 - Pf6N2E2_1328 0.22 +1.0 1,562,550 - Pf6N2E2_1328 0.36 +0.8 1,562,550 - Pf6N2E2_1328 0.36 +0.5 1,562,588 + Pf6N2E2_1328 0.40 +0.3 1,562,589 - Pf6N2E2_1328 0.40 +1.8 1,562,589 - Pf6N2E2_1328 0.40 +0.3 1,562,860 + Pf6N2E2_1328 0.68 +0.4 1,562,860 + Pf6N2E2_1328 0.68 +1.0 1,562,861 - Pf6N2E2_1328 0.68 +0.4 1,562,861 - Pf6N2E2_1328 0.68 -0.6 1,563,293 + +0.5 1,563,753 - Pf6N2E2_1329 0.25 -1.7 1,563,828 - Pf6N2E2_1329 0.30 +0.4 1,564,514 + Pf6N2E2_1329 0.76 -0.7 1,564,514 + Pf6N2E2_1329 0.76 +1.0 1,564,514 + Pf6N2E2_1329 0.76 +0.7 1,564,514 + Pf6N2E2_1329 0.76 +1.1 1,564,514 + Pf6N2E2_1329 0.76 +0.8 1,564,514 + Pf6N2E2_1329 0.76 -1.0 1,564,514 + Pf6N2E2_1329 0.76 -1.3 1,564,515 - Pf6N2E2_1329 0.76 -1.7 1,564,515 - Pf6N2E2_1329 0.76 +0.2 1,564,515 - Pf6N2E2_1329 0.76 -1.2 1,564,605 - Pf6N2E2_1329 0.83 -1.2 1,564,829 + +0.9 1,564,829 + -1.0 1,564,829 + +0.0 1,565,216 - Pf6N2E2_1330 0.14 -0.3 1,565,327 - Pf6N2E2_1330 0.22 -1.4 1,565,327 - Pf6N2E2_1330 0.22 -0.5 1,565,327 - Pf6N2E2_1330 0.22 +2.2 1,565,389 + Pf6N2E2_1330 0.26 +2.0 1,565,497 + Pf6N2E2_1330 0.33 -1.1 1,565,498 - Pf6N2E2_1330 0.33 +0.5 1,565,646 - Pf6N2E2_1330 0.44 -1.0 1,565,646 - Pf6N2E2_1330 0.44 -0.9 1,565,717 - Pf6N2E2_1330 0.48 -0.3 1,565,810 + Pf6N2E2_1330 0.55 +1.1
Or see this region's nucleotide sequence