Experiment: LB 25C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3497 and rpoZ are separated by 59 nucleotides rpoZ and Shew_3499 are separated by 13 nucleotides Shew_3499 and Shew_3500 are separated by 34 nucleotides Shew_3500 and Shew_3501 are separated by 68 nucleotides
Shew_3497: Shew_3497 - guanylate kinase (RefSeq), at 4,183,670 to 4,184,293
_3497
Shew_3498: rpoZ - DNA-directed RNA polymerase subunit omega (RefSeq), at 4,184,353 to 4,184,634
rpoZ
Shew_3499: Shew_3499 - bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (RefSeq), at 4,184,648 to 4,186,753
_3499
Shew_3500: Shew_3500 - putative endoribonuclease L-PSP (RefSeq), at 4,186,788 to 4,187,171
_3500
Shew_3501: Shew_3501 - tRNA guanosine-2'-O-methyltransferase (RefSeq), at 4,187,240 to 4,187,944
_3501
Position (kb) 4184
4185
4186
4187 Strain fitness (log2 ratio) -2
-1
0
1 at 4184.446 kb on + strand, within rpoZ at 4184.556 kb on + strand, within rpoZ at 4186.169 kb on - strand, within Shew_3499 at 4186.480 kb on + strand, within Shew_3499 at 4186.560 kb on - strand at 4187.011 kb on - strand, within Shew_3500 at 4187.095 kb on - strand, within Shew_3500 at 4187.449 kb on + strand, within Shew_3501
Per-strain Table
Position Strand Gene LocusTag Fraction LB 25C remove 4,184,446 + rpoZ Shew_3498 0.33 -0.8 4,184,556 + rpoZ Shew_3498 0.72 -0.8 4,186,169 - Shew_3499 0.72 -2.1 4,186,480 + Shew_3499 0.87 -1.9 4,186,560 - -2.0 4,187,011 - Shew_3500 0.58 -0.2 4,187,095 - Shew_3500 0.80 -0.2 4,187,449 + Shew_3501 0.30 +0.1
Or see this region's nucleotide sequence