Experiment: LB 25C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0215 and Shew_0216 are separated by 52 nucleotides Shew_0216 and Shew_0217 are separated by 74 nucleotides Shew_0217 and Shew_0218 are separated by 8 nucleotides Shew_0218 and Shew_0219 are separated by 211 nucleotides
Shew_0215: Shew_0215 - sporulation domain-containing protein (RefSeq), at 230,408 to 231,943
_0215
Shew_0216: Shew_0216 - DNA adenine methylase (RefSeq), at 231,996 to 232,835
_0216
Shew_0217: Shew_0217 - hypothetical protein (RefSeq), at 232,910 to 233,083
_0217
Shew_0218: Shew_0218 - hypothetical protein (RefSeq), at 233,092 to 233,436
_0218
Shew_0219: Shew_0219 - ribulose-phosphate 3-epimerase (RefSeq), at 233,648 to 234,322
_0219
Position (kb) 231
232
233 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 231.046 kb on - strand, within Shew_0215 at 231.287 kb on - strand, within Shew_0215 at 231.450 kb on + strand, within Shew_0215 at 231.463 kb on + strand, within Shew_0215 at 231.577 kb on + strand, within Shew_0215 at 231.580 kb on - strand, within Shew_0215 at 231.945 kb on + strand at 232.439 kb on + strand, within Shew_0216 at 232.497 kb on - strand, within Shew_0216 at 232.877 kb on + strand at 232.981 kb on + strand, within Shew_0217 at 233.086 kb on - strand at 233.147 kb on + strand, within Shew_0218 at 233.194 kb on + strand, within Shew_0218 at 233.370 kb on - strand, within Shew_0218 at 233.425 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 25C remove 231,046 - Shew_0215 0.42 -0.0 231,287 - Shew_0215 0.57 +0.1 231,450 + Shew_0215 0.68 -0.0 231,463 + Shew_0215 0.69 +0.2 231,577 + Shew_0215 0.76 -0.3 231,580 - Shew_0215 0.76 -0.0 231,945 + -0.5 232,439 + Shew_0216 0.53 -1.4 232,497 - Shew_0216 0.60 -4.7 232,877 + +0.1 232,981 + Shew_0217 0.41 +0.0 233,086 - -0.0 233,147 + Shew_0218 0.16 +0.3 233,194 + Shew_0218 0.30 +0.0 233,370 - Shew_0218 0.81 -0.4 233,425 - -0.2
Or see this region's nucleotide sequence