Strain Fitness in Sinorhizobium meliloti 1021 around SMa2141

Experiment: Min media with glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSMa2139 and SMa2141 are separated by 93 nucleotidesSMa2141 and SMa2143 overlap by 4 nucleotides SMa2139: SMa2139 - SgaA serine-glyoxylate aminotransferase (SGAT), at 1,208,866 to 1,210,071 SMa2139 SMa2141: SMa2141 - formyltetrahydrofolate deformylase, at 1,210,165 to 1,211,025 SMa2141 SMa2143: SMa2143 - 5,10-methylenetetrahydrofolate reductase, at 1,211,022 to 1,211,975 SMa2143 Position (kb) 1210 1211 1212Strain fitness (log2 ratio) -1 0 1 2 3at 1209.322 kb on + strand, within SMa2139at 1209.339 kb on + strand, within SMa2139at 1209.339 kb on + strand, within SMa2139at 1209.339 kb on + strand, within SMa2139at 1209.342 kb on - strand, within SMa2139at 1209.376 kb on - strand, within SMa2139at 1209.476 kb on - strand, within SMa2139at 1210.059 kb on + strandat 1210.355 kb on + strand, within SMa2141at 1210.355 kb on + strand, within SMa2141at 1210.506 kb on - strand, within SMa2141at 1210.608 kb on - strand, within SMa2141at 1210.905 kb on - strand, within SMa2141at 1210.934 kb on - strand, within SMa2141at 1211.132 kb on + strand, within SMa2143at 1211.133 kb on - strand, within SMa2143at 1211.221 kb on - strand, within SMa2143at 1211.221 kb on - strand, within SMa2143at 1211.278 kb on - strand, within SMa2143at 1211.435 kb on + strand, within SMa2143at 1211.517 kb on - strand, within SMa2143at 1211.647 kb on + strand, within SMa2143at 1211.647 kb on + strand, within SMa2143at 1211.648 kb on - strand, within SMa2143at 1211.712 kb on - strand, within SMa2143at 1211.848 kb on - strand, within SMa2143at 1211.886 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Min media with glucose
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1,209,322 + SMa2139 0.38 -0.7
1,209,339 + SMa2139 0.39 +0.4
1,209,339 + SMa2139 0.39 -0.3
1,209,339 + SMa2139 0.39 +0.0
1,209,342 - SMa2139 0.39 -0.9
1,209,376 - SMa2139 0.42 +0.0
1,209,476 - SMa2139 0.51 +1.1
1,210,059 + +3.1
1,210,355 + SMa2141 0.22 -0.7
1,210,355 + SMa2141 0.22 +1.1
1,210,506 - SMa2141 0.40 -0.3
1,210,608 - SMa2141 0.51 -1.1
1,210,905 - SMa2141 0.86 -1.2
1,210,934 - SMa2141 0.89 -0.6
1,211,132 + SMa2143 0.12 -0.3
1,211,133 - SMa2143 0.12 +0.3
1,211,221 - SMa2143 0.21 +0.5
1,211,221 - SMa2143 0.21 -0.4
1,211,278 - SMa2143 0.27 +0.6
1,211,435 + SMa2143 0.43 -0.6
1,211,517 - SMa2143 0.52 -0.4
1,211,647 + SMa2143 0.66 +0.7
1,211,647 + SMa2143 0.66 +0.4
1,211,648 - SMa2143 0.66 -0.3
1,211,712 - SMa2143 0.72 +0.1
1,211,848 - SMa2143 0.87 +2.6
1,211,886 - +0.1

Or see this region's nucleotide sequence