Experiment: periodate 0.00125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_512 and Pf6N2E2_513 are separated by 194 nucleotides Pf6N2E2_513 and Pf6N2E2_514 overlap by 4 nucleotides Pf6N2E2_514 and Pf6N2E2_515 are separated by 30 nucleotides
Pf6N2E2_512 - 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) (from data) , at 580,158 to 582,095
_512
Pf6N2E2_513 - Myo-inosose-2 dehydratase (EC 4.2.1.44) (from data) , at 582,290 to 583,183
_513
Pf6N2E2_514 - 5-deoxy-D-glucuronate isomerase (EC 5.3.1.30) (from data) , at 583,180 to 583,989
_514
Pf6N2E2_515 - malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (from data) , at 584,020 to 585,522
_515
Position (kb)
582
583
584 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 581.293 kb on + strand, within Pf6N2E2_512 at 581.500 kb on + strand, within Pf6N2E2_512 at 581.501 kb on - strand, within Pf6N2E2_512 at 581.658 kb on + strand, within Pf6N2E2_512 at 581.658 kb on + strand, within Pf6N2E2_512 at 581.658 kb on + strand, within Pf6N2E2_512 at 581.658 kb on + strand, within Pf6N2E2_512 at 581.659 kb on - strand, within Pf6N2E2_512 at 581.659 kb on - strand, within Pf6N2E2_512 at 581.659 kb on - strand, within Pf6N2E2_512 at 581.746 kb on - strand, within Pf6N2E2_512 at 581.767 kb on - strand, within Pf6N2E2_512 at 581.778 kb on - strand, within Pf6N2E2_512 at 582.099 kb on + strand at 582.100 kb on - strand at 582.100 kb on - strand at 582.263 kb on + strand at 582.263 kb on + strand at 582.264 kb on - strand at 582.264 kb on - strand at 582.264 kb on - strand at 582.275 kb on + strand at 582.275 kb on + strand at 582.275 kb on + strand at 582.275 kb on + strand at 582.275 kb on + strand at 582.275 kb on + strand at 582.275 kb on + strand at 582.276 kb on - strand at 582.276 kb on - strand at 582.276 kb on - strand at 582.276 kb on - strand at 582.276 kb on - strand at 582.276 kb on - strand at 582.473 kb on + strand, within Pf6N2E2_513 at 582.501 kb on - strand, within Pf6N2E2_513 at 582.735 kb on + strand, within Pf6N2E2_513 at 582.747 kb on - strand, within Pf6N2E2_513 at 582.747 kb on - strand, within Pf6N2E2_513 at 582.767 kb on + strand, within Pf6N2E2_513 at 582.768 kb on - strand, within Pf6N2E2_513 at 582.851 kb on + strand, within Pf6N2E2_513 at 582.851 kb on + strand, within Pf6N2E2_513 at 582.851 kb on + strand, within Pf6N2E2_513 at 582.851 kb on + strand, within Pf6N2E2_513 at 582.851 kb on + strand, within Pf6N2E2_513 at 582.852 kb on - strand, within Pf6N2E2_513 at 582.852 kb on - strand, within Pf6N2E2_513 at 582.852 kb on - strand, within Pf6N2E2_513 at 582.852 kb on - strand, within Pf6N2E2_513 at 582.852 kb on - strand, within Pf6N2E2_513 at 582.852 kb on - strand, within Pf6N2E2_513 at 582.852 kb on - strand, within Pf6N2E2_513 at 583.064 kb on + strand, within Pf6N2E2_513 at 583.107 kb on + strand at 583.108 kb on - strand at 583.118 kb on + strand at 583.118 kb on + strand at 583.118 kb on + strand at 583.118 kb on + strand at 583.119 kb on - strand at 583.119 kb on - strand at 583.140 kb on - strand at 583.249 kb on + strand at 583.249 kb on + strand at 583.249 kb on + strand at 583.250 kb on - strand at 583.250 kb on - strand at 583.435 kb on + strand, within Pf6N2E2_514 at 583.436 kb on - strand, within Pf6N2E2_514 at 583.490 kb on + strand, within Pf6N2E2_514 at 583.491 kb on - strand, within Pf6N2E2_514 at 583.534 kb on + strand, within Pf6N2E2_514 at 583.535 kb on - strand, within Pf6N2E2_514 at 583.535 kb on - strand, within Pf6N2E2_514 at 583.604 kb on - strand, within Pf6N2E2_514 at 583.750 kb on + strand, within Pf6N2E2_514 at 583.750 kb on + strand at 583.751 kb on - strand, within Pf6N2E2_514 at 583.751 kb on - strand, within Pf6N2E2_514 at 583.798 kb on + strand, within Pf6N2E2_514 at 583.798 kb on + strand, within Pf6N2E2_514 at 583.799 kb on - strand, within Pf6N2E2_514 at 583.799 kb on - strand, within Pf6N2E2_514 at 583.906 kb on + strand, within Pf6N2E2_514 at 583.907 kb on - strand, within Pf6N2E2_514 at 583.907 kb on - strand, within Pf6N2E2_514 at 583.909 kb on + strand at 584.003 kb on + strand at 584.047 kb on + strand at 584.047 kb on + strand at 584.048 kb on - strand at 584.048 kb on - strand at 584.048 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction periodate 0.00125 M remove 581,293 + Pf6N2E2_512 0.59 -0.3 581,500 + Pf6N2E2_512 0.69 +0.1 581,501 - Pf6N2E2_512 0.69 -0.1 581,658 + Pf6N2E2_512 0.77 +0.1 581,658 + Pf6N2E2_512 0.77 -0.4 581,658 + Pf6N2E2_512 0.77 +0.3 581,658 + Pf6N2E2_512 0.77 -0.1 581,659 - Pf6N2E2_512 0.77 -0.3 581,659 - Pf6N2E2_512 0.77 -0.8 581,659 - Pf6N2E2_512 0.77 +0.8 581,746 - Pf6N2E2_512 0.82 +1.4 581,767 - Pf6N2E2_512 0.83 +1.8 581,778 - Pf6N2E2_512 0.84 +0.8 582,099 + -0.1 582,100 - +0.1 582,100 - +0.2 582,263 + +1.8 582,263 + +0.2 582,264 - -0.2 582,264 - +0.4 582,264 - +1.2 582,275 + -0.5 582,275 + -0.6 582,275 + +0.3 582,275 + -0.7 582,275 + +1.1 582,275 + +0.0 582,275 + -0.3 582,276 - +0.1 582,276 - +0.8 582,276 - +1.1 582,276 - +1.2 582,276 - +0.8 582,276 - +0.2 582,473 + Pf6N2E2_513 0.20 -0.5 582,501 - Pf6N2E2_513 0.24 +1.6 582,735 + Pf6N2E2_513 0.50 -0.2 582,747 - Pf6N2E2_513 0.51 +0.0 582,747 - Pf6N2E2_513 0.51 +1.3 582,767 + Pf6N2E2_513 0.53 +0.4 582,768 - Pf6N2E2_513 0.53 +0.1 582,851 + Pf6N2E2_513 0.63 +1.2 582,851 + Pf6N2E2_513 0.63 +0.7 582,851 + Pf6N2E2_513 0.63 +1.2 582,851 + Pf6N2E2_513 0.63 +3.2 582,851 + Pf6N2E2_513 0.63 +0.3 582,852 - Pf6N2E2_513 0.63 -0.2 582,852 - Pf6N2E2_513 0.63 +0.4 582,852 - Pf6N2E2_513 0.63 -0.0 582,852 - Pf6N2E2_513 0.63 +0.3 582,852 - Pf6N2E2_513 0.63 +0.9 582,852 - Pf6N2E2_513 0.63 +0.2 582,852 - Pf6N2E2_513 0.63 +0.8 583,064 + Pf6N2E2_513 0.87 -1.6 583,107 + +0.6 583,108 - +0.6 583,118 + +0.0 583,118 + -0.1 583,118 + +1.2 583,118 + -1.2 583,119 - +0.2 583,119 - -0.1 583,140 - -0.9 583,249 + -0.5 583,249 + -1.9 583,249 + +0.2 583,250 - +0.5 583,250 - -0.3 583,435 + Pf6N2E2_514 0.31 +0.9 583,436 - Pf6N2E2_514 0.32 -2.3 583,490 + Pf6N2E2_514 0.38 +0.0 583,491 - Pf6N2E2_514 0.38 -1.5 583,534 + Pf6N2E2_514 0.44 +0.2 583,535 - Pf6N2E2_514 0.44 +0.2 583,535 - Pf6N2E2_514 0.44 +0.5 583,604 - Pf6N2E2_514 0.52 -0.5 583,750 + Pf6N2E2_514 0.70 +0.4 583,750 + +0.8 583,751 - Pf6N2E2_514 0.70 -0.2 583,751 - Pf6N2E2_514 0.70 +0.8 583,798 + Pf6N2E2_514 0.76 +0.4 583,798 + Pf6N2E2_514 0.76 +0.0 583,799 - Pf6N2E2_514 0.76 +0.4 583,799 - Pf6N2E2_514 0.76 +0.6 583,906 + Pf6N2E2_514 0.90 +0.6 583,907 - Pf6N2E2_514 0.90 -1.1 583,907 - Pf6N2E2_514 0.90 +1.0 583,909 + -0.9 584,003 + -1.2 584,047 + +0.8 584,047 + +1.9 584,048 - +0.2 584,048 - -0.2 584,048 - +1.2
Or see this region's nucleotide sequence