Experiment: periodate 0.00125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5041 and Pf6N2E2_5042 are separated by 128 nucleotides Pf6N2E2_5042 and Pf6N2E2_5043 are separated by 21 nucleotides Pf6N2E2_5043 and Pf6N2E2_5044 are separated by 169 nucleotides
Pf6N2E2_5041 - Uncharacterized protein conserved in bacteria, at 5,817,920 to 5,818,669
_5041
Pf6N2E2_5042 - FIG00954572: hypothetical protein, at 5,818,798 to 5,819,277
_5042
Pf6N2E2_5043 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 5,819,299 to 5,819,910
_5043
Pf6N2E2_5044 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 5,820,080 to 5,820,532
_5044
Position (kb)
5818
5819
5820 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5817.812 kb on + strand at 5817.812 kb on + strand at 5817.812 kb on + strand at 5817.812 kb on + strand at 5817.813 kb on - strand at 5817.823 kb on + strand at 5817.824 kb on - strand at 5817.891 kb on + strand at 5817.891 kb on + strand at 5817.892 kb on - strand at 5817.892 kb on - strand at 5817.892 kb on - strand at 5817.892 kb on - strand at 5817.895 kb on + strand at 5817.896 kb on - strand at 5817.896 kb on - strand at 5817.907 kb on + strand at 5817.907 kb on + strand at 5817.907 kb on + strand at 5817.907 kb on + strand at 5817.908 kb on - strand at 5818.055 kb on + strand, within Pf6N2E2_5041 at 5818.056 kb on - strand, within Pf6N2E2_5041 at 5818.349 kb on + strand, within Pf6N2E2_5041 at 5818.349 kb on + strand, within Pf6N2E2_5041 at 5818.350 kb on - strand, within Pf6N2E2_5041 at 5818.586 kb on + strand, within Pf6N2E2_5041 at 5818.587 kb on - strand, within Pf6N2E2_5041 at 5818.587 kb on - strand, within Pf6N2E2_5041 at 5818.622 kb on + strand at 5818.623 kb on - strand at 5818.623 kb on - strand at 5818.711 kb on - strand at 5818.740 kb on - strand at 5818.740 kb on - strand at 5818.740 kb on - strand at 5818.837 kb on + strand at 5818.837 kb on + strand at 5818.838 kb on - strand at 5818.838 kb on - strand at 5818.840 kb on + strand at 5818.840 kb on + strand at 5818.840 kb on + strand at 5818.840 kb on + strand at 5818.841 kb on - strand at 5818.841 kb on - strand at 5818.841 kb on - strand at 5818.841 kb on - strand at 5818.841 kb on - strand at 5818.841 kb on - strand at 5818.841 kb on - strand at 5818.859 kb on - strand, within Pf6N2E2_5042 at 5818.876 kb on + strand, within Pf6N2E2_5042 at 5818.927 kb on + strand, within Pf6N2E2_5042 at 5818.927 kb on + strand, within Pf6N2E2_5042 at 5818.928 kb on - strand, within Pf6N2E2_5042 at 5818.928 kb on - strand, within Pf6N2E2_5042 at 5818.928 kb on - strand, within Pf6N2E2_5042 at 5818.928 kb on - strand, within Pf6N2E2_5042 at 5818.928 kb on - strand, within Pf6N2E2_5042 at 5818.928 kb on - strand, within Pf6N2E2_5042 at 5818.934 kb on - strand, within Pf6N2E2_5042 at 5819.017 kb on + strand, within Pf6N2E2_5042 at 5819.017 kb on + strand, within Pf6N2E2_5042 at 5819.017 kb on + strand, within Pf6N2E2_5042 at 5819.017 kb on + strand, within Pf6N2E2_5042 at 5819.017 kb on + strand, within Pf6N2E2_5042 at 5819.017 kb on + strand, within Pf6N2E2_5042 at 5819.018 kb on - strand, within Pf6N2E2_5042 at 5819.018 kb on - strand, within Pf6N2E2_5042 at 5819.018 kb on - strand, within Pf6N2E2_5042 at 5819.018 kb on - strand, within Pf6N2E2_5042 at 5819.018 kb on - strand, within Pf6N2E2_5042 at 5819.018 kb on - strand at 5819.188 kb on + strand, within Pf6N2E2_5042 at 5819.908 kb on + strand at 5819.908 kb on + strand at 5819.909 kb on - strand at 5819.909 kb on - strand at 5819.909 kb on - strand at 5820.081 kb on + strand at 5820.081 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction periodate 0.00125 M remove 5,817,812 + +0.9 5,817,812 + +1.1 5,817,812 + +0.6 5,817,812 + -1.6 5,817,813 - +0.7 5,817,823 + +0.3 5,817,824 - -0.1 5,817,891 + +1.6 5,817,891 + +0.6 5,817,892 - +1.4 5,817,892 - +0.1 5,817,892 - -0.0 5,817,892 - +0.8 5,817,895 + -1.0 5,817,896 - +0.5 5,817,896 - +1.5 5,817,907 + +0.3 5,817,907 + +2.5 5,817,907 + -0.7 5,817,907 + -1.8 5,817,908 - -0.9 5,818,055 + Pf6N2E2_5041 0.18 -2.3 5,818,056 - Pf6N2E2_5041 0.18 -0.6 5,818,349 + Pf6N2E2_5041 0.57 -0.3 5,818,349 + Pf6N2E2_5041 0.57 -0.6 5,818,350 - Pf6N2E2_5041 0.57 -0.7 5,818,586 + Pf6N2E2_5041 0.89 +1.0 5,818,587 - Pf6N2E2_5041 0.89 -0.4 5,818,587 - Pf6N2E2_5041 0.89 -1.5 5,818,622 + -1.0 5,818,623 - +0.2 5,818,623 - +0.2 5,818,711 - -1.1 5,818,740 - -1.6 5,818,740 - +0.9 5,818,740 - -1.7 5,818,837 + -1.9 5,818,837 + +1.7 5,818,838 - -0.2 5,818,838 - -0.4 5,818,840 + +0.4 5,818,840 + -1.7 5,818,840 + -0.7 5,818,840 + +0.5 5,818,841 - +0.7 5,818,841 - -0.1 5,818,841 - +1.8 5,818,841 - -0.3 5,818,841 - -0.9 5,818,841 - +0.2 5,818,841 - -1.1 5,818,859 - Pf6N2E2_5042 0.13 +0.2 5,818,876 + Pf6N2E2_5042 0.16 +1.5 5,818,927 + Pf6N2E2_5042 0.27 +0.3 5,818,927 + Pf6N2E2_5042 0.27 -0.1 5,818,928 - Pf6N2E2_5042 0.27 -0.4 5,818,928 - Pf6N2E2_5042 0.27 +1.2 5,818,928 - Pf6N2E2_5042 0.27 +0.1 5,818,928 - Pf6N2E2_5042 0.27 +1.7 5,818,928 - Pf6N2E2_5042 0.27 -1.2 5,818,928 - Pf6N2E2_5042 0.27 +0.8 5,818,934 - Pf6N2E2_5042 0.28 -2.3 5,819,017 + Pf6N2E2_5042 0.46 +0.9 5,819,017 + Pf6N2E2_5042 0.46 +0.3 5,819,017 + Pf6N2E2_5042 0.46 +0.5 5,819,017 + Pf6N2E2_5042 0.46 +1.4 5,819,017 + Pf6N2E2_5042 0.46 +0.2 5,819,017 + Pf6N2E2_5042 0.46 -0.1 5,819,018 - Pf6N2E2_5042 0.46 -2.5 5,819,018 - Pf6N2E2_5042 0.46 -2.6 5,819,018 - Pf6N2E2_5042 0.46 +0.3 5,819,018 - Pf6N2E2_5042 0.46 +0.9 5,819,018 - Pf6N2E2_5042 0.46 +0.4 5,819,018 - +0.9 5,819,188 + Pf6N2E2_5042 0.81 +0.1 5,819,908 + +0.4 5,819,908 + -0.0 5,819,909 - +0.6 5,819,909 - +2.8 5,819,909 - -0.4 5,820,081 + -1.7 5,820,081 + +1.6
Or see this region's nucleotide sequence