Experiment: periodate 0.00125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_297 and Pf6N2E2_298 are separated by 57 nucleotides Pf6N2E2_298 and Pf6N2E2_299 are separated by 103 nucleotides Pf6N2E2_299 and Pf6N2E2_300 overlap by 4 nucleotides Pf6N2E2_300 and Pf6N2E2_301 are separated by 12 nucleotides
Pf6N2E2_297 - Cell division protein FtsK, at 366,212 to 368,617
_297
Pf6N2E2_298 - Outer membrane lipoprotein carrier protein LolA, at 368,675 to 369,298
_298
Pf6N2E2_299 - FIG065221: Holliday junction DNA helicase, at 369,402 to 370,727
_299
Pf6N2E2_300 - CrcB protein, at 370,724 to 371,101
_300
Pf6N2E2_301 - Seryl-tRNA synthetase (EC 6.1.1.11), at 371,114 to 372,394
_301
Position (kb) 369
370
371 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 369.360 kb on - strand at 369.360 kb on - strand at 369.394 kb on - strand at 369.394 kb on - strand at 369.394 kb on - strand at 369.397 kb on + strand at 369.397 kb on + strand at 369.397 kb on + strand at 369.397 kb on + strand at 369.398 kb on - strand at 369.474 kb on + strand at 369.475 kb on - strand at 369.475 kb on - strand at 369.780 kb on + strand, within Pf6N2E2_299 at 369.780 kb on + strand, within Pf6N2E2_299 at 369.873 kb on + strand, within Pf6N2E2_299 at 369.873 kb on + strand, within Pf6N2E2_299 at 369.873 kb on + strand, within Pf6N2E2_299 at 369.873 kb on + strand, within Pf6N2E2_299 at 369.874 kb on - strand, within Pf6N2E2_299 at 369.874 kb on - strand, within Pf6N2E2_299 at 369.874 kb on - strand, within Pf6N2E2_299 at 369.874 kb on - strand, within Pf6N2E2_299 at 369.874 kb on - strand, within Pf6N2E2_299 at 369.874 kb on - strand, within Pf6N2E2_299 at 370.207 kb on - strand, within Pf6N2E2_299 at 370.248 kb on + strand, within Pf6N2E2_299 at 370.248 kb on + strand, within Pf6N2E2_299 at 370.249 kb on - strand, within Pf6N2E2_299 at 370.395 kb on + strand, within Pf6N2E2_299 at 370.395 kb on + strand, within Pf6N2E2_299 at 370.395 kb on + strand, within Pf6N2E2_299 at 370.396 kb on - strand, within Pf6N2E2_299 at 370.396 kb on - strand, within Pf6N2E2_299 at 370.428 kb on + strand, within Pf6N2E2_299 at 370.429 kb on - strand, within Pf6N2E2_299 at 370.429 kb on - strand, within Pf6N2E2_299 at 370.552 kb on - strand, within Pf6N2E2_299 at 370.575 kb on + strand, within Pf6N2E2_299 at 370.575 kb on + strand, within Pf6N2E2_299 at 370.575 kb on + strand, within Pf6N2E2_299 at 370.593 kb on + strand, within Pf6N2E2_299 at 370.594 kb on - strand, within Pf6N2E2_299 at 370.626 kb on + strand at 370.627 kb on - strand at 370.826 kb on + strand, within Pf6N2E2_300 at 370.826 kb on + strand, within Pf6N2E2_300 at 370.826 kb on + strand, within Pf6N2E2_300 at 370.826 kb on + strand, within Pf6N2E2_300 at 370.846 kb on + strand, within Pf6N2E2_300 at 370.849 kb on + strand, within Pf6N2E2_300 at 370.849 kb on + strand, within Pf6N2E2_300 at 370.849 kb on + strand, within Pf6N2E2_300 at 371.099 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction periodate 0.00125 M remove 369,360 - -0.6 369,360 - +1.8 369,394 - -0.8 369,394 - -2.0 369,394 - -0.8 369,397 + -1.7 369,397 + -1.4 369,397 + +0.7 369,397 + +0.8 369,398 - -2.0 369,474 + -1.4 369,475 - +0.2 369,475 - -0.2 369,780 + Pf6N2E2_299 0.29 -1.0 369,780 + Pf6N2E2_299 0.29 -1.2 369,873 + Pf6N2E2_299 0.36 +0.4 369,873 + Pf6N2E2_299 0.36 -0.8 369,873 + Pf6N2E2_299 0.36 -1.4 369,873 + Pf6N2E2_299 0.36 -1.9 369,874 - Pf6N2E2_299 0.36 -0.2 369,874 - Pf6N2E2_299 0.36 -1.4 369,874 - Pf6N2E2_299 0.36 -3.0 369,874 - Pf6N2E2_299 0.36 -1.0 369,874 - Pf6N2E2_299 0.36 -1.0 369,874 - Pf6N2E2_299 0.36 -0.5 370,207 - Pf6N2E2_299 0.61 -1.4 370,248 + Pf6N2E2_299 0.64 +0.2 370,248 + Pf6N2E2_299 0.64 -1.4 370,249 - Pf6N2E2_299 0.64 -1.3 370,395 + Pf6N2E2_299 0.75 -1.8 370,395 + Pf6N2E2_299 0.75 -2.2 370,395 + Pf6N2E2_299 0.75 -0.7 370,396 - Pf6N2E2_299 0.75 +2.8 370,396 - Pf6N2E2_299 0.75 -2.4 370,428 + Pf6N2E2_299 0.77 -2.3 370,429 - Pf6N2E2_299 0.77 -1.4 370,429 - Pf6N2E2_299 0.77 -2.4 370,552 - Pf6N2E2_299 0.87 -1.2 370,575 + Pf6N2E2_299 0.88 -1.8 370,575 + Pf6N2E2_299 0.88 +0.4 370,575 + Pf6N2E2_299 0.88 -1.8 370,593 + Pf6N2E2_299 0.90 -0.3 370,594 - Pf6N2E2_299 0.90 -1.8 370,626 + -2.6 370,627 - -1.4 370,826 + Pf6N2E2_300 0.27 -0.8 370,826 + Pf6N2E2_300 0.27 +0.8 370,826 + Pf6N2E2_300 0.27 -0.1 370,826 + Pf6N2E2_300 0.27 -1.2 370,846 + Pf6N2E2_300 0.32 -2.2 370,849 + Pf6N2E2_300 0.33 -1.4 370,849 + Pf6N2E2_300 0.33 -0.7 370,849 + Pf6N2E2_300 0.33 +0.4 371,099 + +0.7
Or see this region's nucleotide sequence