Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_299

Experiment: periodate 0.00125 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_297 and Pf6N2E2_298 are separated by 57 nucleotidesPf6N2E2_298 and Pf6N2E2_299 are separated by 103 nucleotidesPf6N2E2_299 and Pf6N2E2_300 overlap by 4 nucleotidesPf6N2E2_300 and Pf6N2E2_301 are separated by 12 nucleotides Pf6N2E2_297 - Cell division protein FtsK, at 366,212 to 368,617 _297 Pf6N2E2_298 - Outer membrane lipoprotein carrier protein LolA, at 368,675 to 369,298 _298 Pf6N2E2_299 - FIG065221: Holliday junction DNA helicase, at 369,402 to 370,727 _299 Pf6N2E2_300 - CrcB protein, at 370,724 to 371,101 _300 Pf6N2E2_301 - Seryl-tRNA synthetase (EC 6.1.1.11), at 371,114 to 372,394 _301 Position (kb) 369 370 371Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 369.360 kb on - strandat 369.360 kb on - strandat 369.394 kb on - strandat 369.394 kb on - strandat 369.394 kb on - strandat 369.397 kb on + strandat 369.397 kb on + strandat 369.397 kb on + strandat 369.397 kb on + strandat 369.398 kb on - strandat 369.474 kb on + strandat 369.475 kb on - strandat 369.475 kb on - strandat 369.780 kb on + strand, within Pf6N2E2_299at 369.780 kb on + strand, within Pf6N2E2_299at 369.873 kb on + strand, within Pf6N2E2_299at 369.873 kb on + strand, within Pf6N2E2_299at 369.873 kb on + strand, within Pf6N2E2_299at 369.873 kb on + strand, within Pf6N2E2_299at 369.874 kb on - strand, within Pf6N2E2_299at 369.874 kb on - strand, within Pf6N2E2_299at 369.874 kb on - strand, within Pf6N2E2_299at 369.874 kb on - strand, within Pf6N2E2_299at 369.874 kb on - strand, within Pf6N2E2_299at 369.874 kb on - strand, within Pf6N2E2_299at 370.207 kb on - strand, within Pf6N2E2_299at 370.248 kb on + strand, within Pf6N2E2_299at 370.248 kb on + strand, within Pf6N2E2_299at 370.249 kb on - strand, within Pf6N2E2_299at 370.395 kb on + strand, within Pf6N2E2_299at 370.395 kb on + strand, within Pf6N2E2_299at 370.395 kb on + strand, within Pf6N2E2_299at 370.396 kb on - strand, within Pf6N2E2_299at 370.396 kb on - strand, within Pf6N2E2_299at 370.428 kb on + strand, within Pf6N2E2_299at 370.429 kb on - strand, within Pf6N2E2_299at 370.429 kb on - strand, within Pf6N2E2_299at 370.552 kb on - strand, within Pf6N2E2_299at 370.575 kb on + strand, within Pf6N2E2_299at 370.575 kb on + strand, within Pf6N2E2_299at 370.575 kb on + strand, within Pf6N2E2_299at 370.593 kb on + strand, within Pf6N2E2_299at 370.594 kb on - strand, within Pf6N2E2_299at 370.626 kb on + strandat 370.627 kb on - strandat 370.826 kb on + strand, within Pf6N2E2_300at 370.826 kb on + strand, within Pf6N2E2_300at 370.826 kb on + strand, within Pf6N2E2_300at 370.826 kb on + strand, within Pf6N2E2_300at 370.846 kb on + strand, within Pf6N2E2_300at 370.849 kb on + strand, within Pf6N2E2_300at 370.849 kb on + strand, within Pf6N2E2_300at 370.849 kb on + strand, within Pf6N2E2_300at 371.099 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction periodate 0.00125 M
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369,360 - -0.6
369,360 - +1.8
369,394 - -0.8
369,394 - -2.0
369,394 - -0.8
369,397 + -1.7
369,397 + -1.4
369,397 + +0.7
369,397 + +0.8
369,398 - -2.0
369,474 + -1.4
369,475 - +0.2
369,475 - -0.2
369,780 + Pf6N2E2_299 0.29 -1.0
369,780 + Pf6N2E2_299 0.29 -1.2
369,873 + Pf6N2E2_299 0.36 +0.4
369,873 + Pf6N2E2_299 0.36 -0.8
369,873 + Pf6N2E2_299 0.36 -1.4
369,873 + Pf6N2E2_299 0.36 -1.9
369,874 - Pf6N2E2_299 0.36 -0.2
369,874 - Pf6N2E2_299 0.36 -1.4
369,874 - Pf6N2E2_299 0.36 -3.0
369,874 - Pf6N2E2_299 0.36 -1.0
369,874 - Pf6N2E2_299 0.36 -1.0
369,874 - Pf6N2E2_299 0.36 -0.5
370,207 - Pf6N2E2_299 0.61 -1.4
370,248 + Pf6N2E2_299 0.64 +0.2
370,248 + Pf6N2E2_299 0.64 -1.4
370,249 - Pf6N2E2_299 0.64 -1.3
370,395 + Pf6N2E2_299 0.75 -1.8
370,395 + Pf6N2E2_299 0.75 -2.2
370,395 + Pf6N2E2_299 0.75 -0.7
370,396 - Pf6N2E2_299 0.75 +2.8
370,396 - Pf6N2E2_299 0.75 -2.4
370,428 + Pf6N2E2_299 0.77 -2.3
370,429 - Pf6N2E2_299 0.77 -1.4
370,429 - Pf6N2E2_299 0.77 -2.4
370,552 - Pf6N2E2_299 0.87 -1.2
370,575 + Pf6N2E2_299 0.88 -1.8
370,575 + Pf6N2E2_299 0.88 +0.4
370,575 + Pf6N2E2_299 0.88 -1.8
370,593 + Pf6N2E2_299 0.90 -0.3
370,594 - Pf6N2E2_299 0.90 -1.8
370,626 + -2.6
370,627 - -1.4
370,826 + Pf6N2E2_300 0.27 -0.8
370,826 + Pf6N2E2_300 0.27 +0.8
370,826 + Pf6N2E2_300 0.27 -0.1
370,826 + Pf6N2E2_300 0.27 -1.2
370,846 + Pf6N2E2_300 0.32 -2.2
370,849 + Pf6N2E2_300 0.33 -1.4
370,849 + Pf6N2E2_300 0.33 -0.7
370,849 + Pf6N2E2_300 0.33 +0.4
371,099 + +0.7

Or see this region's nucleotide sequence