Experiment: Formate with nitrate 62.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00135 and MMP_RS00140 are separated by 4 nucleotides MMP_RS00140 and MMP_RS00145 overlap by 4 nucleotides MMP_RS00145 and MMP_RS00150 are separated by 179 nucleotides
MMP_RS00135: MMP_RS00135 - DUF2121 domain-containing protein, at 29,531 to 30,406
_RS00135
MMP_RS00140: MMP_RS00140 - DUF192 domain-containing protein, at 30,411 to 30,746
_RS00140
MMP_RS00145: MMP_RS00145 - ATP-dependent Clp protease proteolytic subunit, at 30,743 to 31,567
_RS00145
MMP_RS00150: MMP_RS00150 - AAA family ATPase, at 31,747 to 32,862
_RS00150
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 29.746 kb on + strand, within MMP_RS00135 at 29.770 kb on + strand, within MMP_RS00135 at 29.770 kb on + strand, within MMP_RS00135 at 29.807 kb on - strand, within MMP_RS00135 at 29.807 kb on - strand, within MMP_RS00135 at 30.051 kb on + strand, within MMP_RS00135 at 30.051 kb on + strand, within MMP_RS00135 at 30.231 kb on - strand, within MMP_RS00135 at 30.253 kb on - strand, within MMP_RS00135 at 30.262 kb on + strand, within MMP_RS00135 at 30.262 kb on + strand, within MMP_RS00135 at 30.479 kb on - strand, within MMP_RS00140 at 30.489 kb on - strand, within MMP_RS00140 at 30.520 kb on + strand, within MMP_RS00140 at 30.534 kb on + strand, within MMP_RS00140 at 30.571 kb on + strand, within MMP_RS00140 at 30.703 kb on + strand, within MMP_RS00140 at 30.723 kb on - strand at 30.780 kb on - strand at 30.780 kb on - strand at 30.780 kb on - strand at 30.841 kb on - strand, within MMP_RS00145 at 30.890 kb on + strand, within MMP_RS00145 at 30.926 kb on + strand, within MMP_RS00145 at 31.128 kb on + strand, within MMP_RS00145 at 31.278 kb on + strand, within MMP_RS00145 at 31.319 kb on + strand, within MMP_RS00145 at 31.319 kb on + strand, within MMP_RS00145 at 31.337 kb on + strand, within MMP_RS00145 at 31.372 kb on - strand, within MMP_RS00145 at 31.377 kb on - strand, within MMP_RS00145 at 31.378 kb on + strand, within MMP_RS00145 at 31.378 kb on + strand, within MMP_RS00145 at 31.382 kb on + strand, within MMP_RS00145 at 31.451 kb on + strand, within MMP_RS00145 at 31.498 kb on + strand at 31.501 kb on - strand at 31.615 kb on + strand at 31.615 kb on + strand at 31.623 kb on - strand at 31.623 kb on - strand at 31.624 kb on + strand at 31.624 kb on + strand at 31.627 kb on - strand at 31.632 kb on - strand at 31.637 kb on - strand at 31.637 kb on - strand at 31.637 kb on - strand at 31.638 kb on + strand at 31.638 kb on + strand at 31.638 kb on + strand at 31.638 kb on + strand at 31.638 kb on + strand at 31.639 kb on - strand at 31.639 kb on - strand at 31.639 kb on - strand at 31.639 kb on - strand at 31.639 kb on - strand at 31.640 kb on + strand at 31.640 kb on + strand at 31.641 kb on - strand at 31.642 kb on + strand at 31.642 kb on + strand at 31.642 kb on + strand at 31.643 kb on - strand at 31.644 kb on + strand at 31.658 kb on + strand at 31.667 kb on - strand at 31.667 kb on - strand at 31.667 kb on - strand at 31.667 kb on - strand at 31.667 kb on - strand at 31.667 kb on - strand at 31.667 kb on - strand at 31.668 kb on + strand at 31.668 kb on + strand at 31.668 kb on + strand at 31.671 kb on - strand at 31.671 kb on - strand at 31.672 kb on + strand at 31.675 kb on - strand at 31.675 kb on - strand at 31.681 kb on - strand at 31.685 kb on - strand at 31.729 kb on - strand at 31.729 kb on - strand at 31.730 kb on + strand at 31.730 kb on + strand at 31.834 kb on - strand at 32.007 kb on - strand, within MMP_RS00150 at 32.061 kb on + strand, within MMP_RS00150 at 32.065 kb on + strand, within MMP_RS00150 at 32.176 kb on - strand, within MMP_RS00150 at 32.225 kb on - strand, within MMP_RS00150 at 32.304 kb on + strand, within MMP_RS00150 at 32.304 kb on + strand, within MMP_RS00150 at 32.304 kb on + strand, within MMP_RS00150 at 32.402 kb on - strand, within MMP_RS00150 at 32.474 kb on - strand, within MMP_RS00150 at 32.475 kb on + strand, within MMP_RS00150
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with nitrate 62.5 mM remove 29,746 + MMP_RS00135 0.25 +1.3 29,770 + MMP_RS00135 0.27 +0.4 29,770 + MMP_RS00135 0.27 -0.5 29,807 - MMP_RS00135 0.32 +1.0 29,807 - MMP_RS00135 0.32 +0.9 30,051 + MMP_RS00135 0.59 +0.7 30,051 + MMP_RS00135 0.59 -0.7 30,231 - MMP_RS00135 0.80 +0.4 30,253 - MMP_RS00135 0.82 +0.1 30,262 + MMP_RS00135 0.83 +0.7 30,262 + MMP_RS00135 0.83 +0.4 30,479 - MMP_RS00140 0.20 +0.6 30,489 - MMP_RS00140 0.23 +0.0 30,520 + MMP_RS00140 0.32 +0.3 30,534 + MMP_RS00140 0.37 -1.5 30,571 + MMP_RS00140 0.48 -0.3 30,703 + MMP_RS00140 0.87 +0.5 30,723 - -0.3 30,780 - -0.9 30,780 - -0.8 30,780 - -0.5 30,841 - MMP_RS00145 0.12 -1.1 30,890 + MMP_RS00145 0.18 -0.3 30,926 + MMP_RS00145 0.22 -0.7 31,128 + MMP_RS00145 0.47 +0.8 31,278 + MMP_RS00145 0.65 -2.3 31,319 + MMP_RS00145 0.70 -1.4 31,319 + MMP_RS00145 0.70 -1.7 31,337 + MMP_RS00145 0.72 -1.9 31,372 - MMP_RS00145 0.76 -2.1 31,377 - MMP_RS00145 0.77 -0.3 31,378 + MMP_RS00145 0.77 -0.7 31,378 + MMP_RS00145 0.77 +0.0 31,382 + MMP_RS00145 0.77 +0.2 31,451 + MMP_RS00145 0.86 -0.5 31,498 + -1.8 31,501 - -0.7 31,615 + -0.5 31,615 + -0.5 31,623 - -0.2 31,623 - -2.1 31,624 + -1.0 31,624 + -2.5 31,627 - -3.1 31,632 - -2.0 31,637 - -0.9 31,637 - -1.1 31,637 - -0.7 31,638 + -0.1 31,638 + -0.9 31,638 + -3.0 31,638 + -1.4 31,638 + -0.4 31,639 - -0.7 31,639 - -1.3 31,639 - -0.9 31,639 - -1.5 31,639 - -0.1 31,640 + -0.5 31,640 + -1.6 31,641 - -0.7 31,642 + -0.1 31,642 + -0.9 31,642 + -1.2 31,643 - +0.0 31,644 + -0.6 31,658 + +0.5 31,667 - -1.2 31,667 - +0.7 31,667 - +0.4 31,667 - -0.6 31,667 - -1.3 31,667 - -0.1 31,667 - +0.2 31,668 + +0.7 31,668 + +0.6 31,668 + -0.0 31,671 - +2.4 31,671 - -0.3 31,672 + +0.5 31,675 - +0.2 31,675 - +0.4 31,681 - +0.5 31,685 - +1.4 31,729 - +0.6 31,729 - +0.3 31,730 + +0.7 31,730 + +0.1 31,834 - -1.4 32,007 - MMP_RS00150 0.23 +0.2 32,061 + MMP_RS00150 0.28 +0.1 32,065 + MMP_RS00150 0.28 +1.5 32,176 - MMP_RS00150 0.38 +0.6 32,225 - MMP_RS00150 0.43 +0.8 32,304 + MMP_RS00150 0.50 +0.1 32,304 + MMP_RS00150 0.50 +0.1 32,304 + MMP_RS00150 0.50 +1.1 32,402 - MMP_RS00150 0.59 +0.8 32,474 - MMP_RS00150 0.65 -0.3 32,475 + MMP_RS00150 0.65 +1.6
Or see this region's nucleotide sequence