Experiment: Recovery broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS19365 and BBR_RS21110 are separated by 293 nucleotides BBR_RS21110 and BBR_RS19370 are separated by 311 nucleotides BBR_RS19370 and BBR_RS19375 are separated by 39 nucleotides
BBR_RS19365: BBR_RS19365 - flavin reductase, at 2,117,950 to 2,118,382
_RS19365
BBR_RS21110: BBR_RS21110 - AraC family transcriptional regulator, at 2,118,676 to 2,118,949
_RS21110
BBR_RS19370: BBR_RS19370 - type I polyketide synthase, at 2,119,261 to 2,128,743
_RS19370
BBR_RS19375: BBR_RS19375 - acyl-CoA carboxylase subunit beta, at 2,128,783 to 2,130,405
_RS19375
Position (kb)
2119
2120
2121
2122
2123
2124
2125
2126
2127
2128
2129 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2118.262 kb on - strand, within BBR_RS19365 at 2118.264 kb on + strand, within BBR_RS19365 at 2118.272 kb on - strand, within BBR_RS19365 at 2118.272 kb on - strand, within BBR_RS19365 at 2118.272 kb on - strand, within BBR_RS19365 at 2118.277 kb on - strand, within BBR_RS19365 at 2118.281 kb on + strand, within BBR_RS19365 at 2118.288 kb on + strand, within BBR_RS19365 at 2118.296 kb on - strand, within BBR_RS19365 at 2118.296 kb on - strand, within BBR_RS19365 at 2118.296 kb on - strand, within BBR_RS19365 at 2118.296 kb on - strand, within BBR_RS19365 at 2118.306 kb on + strand, within BBR_RS19365 at 2118.306 kb on + strand, within BBR_RS19365 at 2118.310 kb on + strand, within BBR_RS19365 at 2118.314 kb on - strand, within BBR_RS19365 at 2118.318 kb on - strand, within BBR_RS19365 at 2118.320 kb on + strand, within BBR_RS19365 at 2118.325 kb on + strand, within BBR_RS19365 at 2118.349 kb on + strand at 2118.377 kb on + strand at 2118.377 kb on + strand at 2118.380 kb on - strand at 2118.380 kb on - strand at 2118.380 kb on - strand at 2118.380 kb on - strand at 2118.380 kb on - strand at 2118.387 kb on - strand at 2118.390 kb on - strand at 2118.401 kb on - strand at 2118.441 kb on + strand at 2118.441 kb on + strand at 2118.444 kb on - strand at 2118.449 kb on - strand at 2118.475 kb on - strand at 2118.515 kb on + strand at 2118.515 kb on + strand at 2118.515 kb on + strand at 2118.515 kb on + strand at 2118.515 kb on + strand at 2118.515 kb on + strand at 2118.515 kb on + strand at 2118.515 kb on + strand at 2118.515 kb on + strand at 2118.517 kb on - strand at 2118.523 kb on - strand at 2118.523 kb on - strand at 2118.524 kb on - strand at 2118.532 kb on + strand at 2118.540 kb on - strand at 2118.540 kb on - strand at 2118.540 kb on - strand at 2118.560 kb on - strand at 2118.597 kb on + strand at 2118.610 kb on - strand at 2118.619 kb on + strand at 2118.620 kb on - strand at 2118.620 kb on - strand at 2118.632 kb on + strand at 2118.641 kb on + strand at 2118.642 kb on + strand at 2118.642 kb on + strand at 2118.656 kb on + strand at 2118.682 kb on - strand at 2118.717 kb on - strand, within BBR_RS21110 at 2118.752 kb on + strand, within BBR_RS21110 at 2118.752 kb on + strand, within BBR_RS21110 at 2118.758 kb on - strand, within BBR_RS21110 at 2118.764 kb on + strand, within BBR_RS21110 at 2118.769 kb on - strand, within BBR_RS21110 at 2118.772 kb on - strand, within BBR_RS21110 at 2118.772 kb on - strand, within BBR_RS21110 at 2118.794 kb on + strand, within BBR_RS21110 at 2118.805 kb on + strand, within BBR_RS21110 at 2118.830 kb on + strand, within BBR_RS21110 at 2118.835 kb on + strand, within BBR_RS21110 at 2118.877 kb on + strand, within BBR_RS21110 at 2118.886 kb on - strand, within BBR_RS21110 at 2118.937 kb on - strand at 2118.956 kb on + strand at 2118.961 kb on + strand at 2118.969 kb on - strand at 2118.969 kb on - strand at 2118.969 kb on - strand at 2118.969 kb on - strand at 2118.972 kb on + strand at 2118.980 kb on + strand at 2119.006 kb on - strand at 2119.015 kb on - strand at 2119.031 kb on + strand at 2119.038 kb on + strand at 2119.085 kb on + strand at 2119.098 kb on + strand at 2119.106 kb on - strand at 2119.137 kb on - strand at 2119.147 kb on - strand at 2119.147 kb on - strand at 2119.160 kb on - strand at 2119.193 kb on + strand at 2119.199 kb on + strand at 2119.233 kb on + strand at 2119.233 kb on + strand at 2119.233 kb on + strand at 2119.233 kb on + strand at 2119.241 kb on - strand at 2119.243 kb on - strand at 2119.248 kb on - strand at 2119.248 kb on - strand at 2119.258 kb on + strand at 2119.259 kb on + strand at 2119.271 kb on + strand at 2119.277 kb on - strand at 2119.468 kb on - strand at 2119.515 kb on - strand at 2119.549 kb on - strand at 2119.777 kb on - strand at 2120.345 kb on - strand, within BBR_RS19370 at 2120.373 kb on - strand, within BBR_RS19370 at 2120.444 kb on + strand, within BBR_RS19370 at 2120.500 kb on - strand, within BBR_RS19370 at 2121.098 kb on + strand, within BBR_RS19370 at 2121.541 kb on - strand, within BBR_RS19370 at 2121.754 kb on + strand, within BBR_RS19370 at 2121.973 kb on - strand, within BBR_RS19370 at 2122.267 kb on - strand, within BBR_RS19370 at 2122.338 kb on - strand, within BBR_RS19370 at 2122.444 kb on - strand, within BBR_RS19370 at 2122.727 kb on + strand, within BBR_RS19370 at 2122.735 kb on - strand, within BBR_RS19370 at 2122.735 kb on - strand, within BBR_RS19370 at 2123.930 kb on + strand, within BBR_RS19370 at 2123.946 kb on - strand, within BBR_RS19370 at 2123.953 kb on - strand, within BBR_RS19370 at 2124.450 kb on + strand, within BBR_RS19370 at 2124.667 kb on + strand, within BBR_RS19370 at 2124.672 kb on + strand, within BBR_RS19370 at 2125.231 kb on - strand, within BBR_RS19370 at 2125.343 kb on + strand, within BBR_RS19370 at 2125.430 kb on + strand, within BBR_RS19370 at 2125.528 kb on - strand, within BBR_RS19370 at 2126.137 kb on + strand, within BBR_RS19370 at 2126.164 kb on + strand, within BBR_RS19370 at 2127.912 kb on + strand at 2127.977 kb on + strand at 2127.979 kb on + strand at 2128.188 kb on + strand at 2128.213 kb on - strand at 2128.283 kb on - strand at 2128.469 kb on - strand at 2128.523 kb on - strand at 2128.773 kb on - strand at 2129.021 kb on - strand, within BBR_RS19375 at 2129.086 kb on + strand, within BBR_RS19375 at 2129.677 kb on + strand, within BBR_RS19375
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth remove 2,118,262 - BBR_RS19365 0.72 +0.0 2,118,264 + BBR_RS19365 0.73 -0.4 2,118,272 - BBR_RS19365 0.74 -0.9 2,118,272 - BBR_RS19365 0.74 -0.3 2,118,272 - BBR_RS19365 0.74 -1.0 2,118,277 - BBR_RS19365 0.76 +1.0 2,118,281 + BBR_RS19365 0.76 -0.6 2,118,288 + BBR_RS19365 0.78 -0.5 2,118,296 - BBR_RS19365 0.80 +0.0 2,118,296 - BBR_RS19365 0.80 +0.0 2,118,296 - BBR_RS19365 0.80 -0.5 2,118,296 - BBR_RS19365 0.80 -1.7 2,118,306 + BBR_RS19365 0.82 +0.2 2,118,306 + BBR_RS19365 0.82 -0.3 2,118,310 + BBR_RS19365 0.83 +0.0 2,118,314 - BBR_RS19365 0.84 +0.3 2,118,318 - BBR_RS19365 0.85 +0.2 2,118,320 + BBR_RS19365 0.85 -0.3 2,118,325 + BBR_RS19365 0.87 -0.4 2,118,349 + +1.8 2,118,377 + -0.9 2,118,377 + -0.8 2,118,380 - +0.4 2,118,380 - +0.5 2,118,380 - -1.3 2,118,380 - +0.3 2,118,380 - +0.2 2,118,387 - +0.5 2,118,390 - +0.9 2,118,401 - -1.0 2,118,441 + +0.5 2,118,441 + +0.1 2,118,444 - +0.9 2,118,449 - -0.1 2,118,475 - -0.2 2,118,515 + +1.1 2,118,515 + +1.2 2,118,515 + -1.9 2,118,515 + +0.2 2,118,515 + +1.0 2,118,515 + +0.1 2,118,515 + -0.3 2,118,515 + +0.2 2,118,515 + +0.4 2,118,517 - -1.7 2,118,523 - +0.6 2,118,523 - +0.8 2,118,524 - -0.6 2,118,532 + -0.0 2,118,540 - +0.3 2,118,540 - -0.2 2,118,540 - -0.4 2,118,560 - -0.2 2,118,597 + +1.3 2,118,610 - -0.2 2,118,619 + +0.1 2,118,620 - +0.1 2,118,620 - +0.1 2,118,632 + +0.1 2,118,641 + +0.3 2,118,642 + -0.5 2,118,642 + -0.7 2,118,656 + +0.1 2,118,682 - -0.9 2,118,717 - BBR_RS21110 0.15 +0.3 2,118,752 + BBR_RS21110 0.28 +0.1 2,118,752 + BBR_RS21110 0.28 +0.2 2,118,758 - BBR_RS21110 0.30 -1.1 2,118,764 + BBR_RS21110 0.32 +0.3 2,118,769 - BBR_RS21110 0.34 -0.3 2,118,772 - BBR_RS21110 0.35 +0.8 2,118,772 - BBR_RS21110 0.35 -0.0 2,118,794 + BBR_RS21110 0.43 -0.8 2,118,805 + BBR_RS21110 0.47 -0.7 2,118,830 + BBR_RS21110 0.56 +0.2 2,118,835 + BBR_RS21110 0.58 -0.2 2,118,877 + BBR_RS21110 0.73 -0.2 2,118,886 - BBR_RS21110 0.77 +0.0 2,118,937 - -0.0 2,118,956 + -0.9 2,118,961 + -0.8 2,118,969 - -0.2 2,118,969 - +0.5 2,118,969 - -0.7 2,118,969 - -0.0 2,118,972 + -1.2 2,118,980 + -0.2 2,119,006 - +0.1 2,119,015 - +0.1 2,119,031 + -0.3 2,119,038 + -0.1 2,119,085 + -0.4 2,119,098 + -0.0 2,119,106 - +0.4 2,119,137 - +0.4 2,119,147 - +0.7 2,119,147 - +0.4 2,119,160 - +0.1 2,119,193 + -0.3 2,119,199 + -0.3 2,119,233 + -0.0 2,119,233 + +0.5 2,119,233 + +0.7 2,119,233 + +0.1 2,119,241 - -1.5 2,119,243 - +0.2 2,119,248 - +0.5 2,119,248 - -0.5 2,119,258 + +0.8 2,119,259 + -0.9 2,119,271 + -0.8 2,119,277 - +0.2 2,119,468 - +3.2 2,119,515 - +0.8 2,119,549 - +1.0 2,119,777 - -0.7 2,120,345 - BBR_RS19370 0.11 +0.5 2,120,373 - BBR_RS19370 0.12 +1.8 2,120,444 + BBR_RS19370 0.12 +0.5 2,120,500 - BBR_RS19370 0.13 +0.6 2,121,098 + BBR_RS19370 0.19 -0.5 2,121,541 - BBR_RS19370 0.24 +1.2 2,121,754 + BBR_RS19370 0.26 +1.6 2,121,973 - BBR_RS19370 0.29 -0.2 2,122,267 - BBR_RS19370 0.32 -0.2 2,122,338 - BBR_RS19370 0.32 +3.0 2,122,444 - BBR_RS19370 0.34 -0.5 2,122,727 + BBR_RS19370 0.37 +0.5 2,122,735 - BBR_RS19370 0.37 +0.6 2,122,735 - BBR_RS19370 0.37 -0.8 2,123,930 + BBR_RS19370 0.49 +0.4 2,123,946 - BBR_RS19370 0.49 +3.5 2,123,953 - BBR_RS19370 0.49 +0.2 2,124,450 + BBR_RS19370 0.55 -0.3 2,124,667 + BBR_RS19370 0.57 +0.6 2,124,672 + BBR_RS19370 0.57 -2.1 2,125,231 - BBR_RS19370 0.63 +1.2 2,125,343 + BBR_RS19370 0.64 +0.8 2,125,430 + BBR_RS19370 0.65 -0.4 2,125,528 - BBR_RS19370 0.66 -0.5 2,126,137 + BBR_RS19370 0.73 +0.8 2,126,164 + BBR_RS19370 0.73 +1.6 2,127,912 + +1.0 2,127,977 + +1.0 2,127,979 + +0.6 2,128,188 + +1.0 2,128,213 - -0.8 2,128,283 - -1.3 2,128,469 - +1.8 2,128,523 - +1.4 2,128,773 - +0.0 2,129,021 - BBR_RS19375 0.15 +0.2 2,129,086 + BBR_RS19375 0.19 -0.5 2,129,677 + BBR_RS19375 0.55 -0.0
Or see this region's nucleotide sequence