Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3130 and Shew_3131 are separated by 81 nucleotides Shew_3131 and Shew_3132 overlap by 1 nucleotides Shew_3132 and Shew_3133 are separated by 95 nucleotides
Shew_3130: Shew_3130 - glutamate--tRNA ligase (RefSeq), at 3,721,930 to 3,722,889
_3130
Shew_3131: Shew_3131 - poly(A) polymerase (RefSeq), at 3,722,971 to 3,724,413
_3131
Shew_3132: Shew_3132 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (RefSeq), at 3,724,413 to 3,724,901
_3132
Shew_3133: Shew_3133 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (RefSeq), at 3,724,997 to 3,725,791
_3133
Position (kb)
3722
3723
3724
3725 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3722.022 kb on + strand at 3722.030 kb on - strand, within Shew_3130 at 3722.156 kb on + strand, within Shew_3130 at 3722.380 kb on + strand, within Shew_3130 at 3722.386 kb on - strand, within Shew_3130 at 3722.737 kb on + strand, within Shew_3130 at 3722.822 kb on - strand at 3722.901 kb on + strand at 3722.991 kb on + strand at 3723.576 kb on + strand, within Shew_3131 at 3723.992 kb on + strand, within Shew_3131 at 3724.239 kb on + strand, within Shew_3131 at 3724.310 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 3,722,022 + +0.2 3,722,030 - Shew_3130 0.10 +0.5 3,722,156 + Shew_3130 0.24 +0.2 3,722,380 + Shew_3130 0.47 +0.3 3,722,386 - Shew_3130 0.47 +0.4 3,722,737 + Shew_3130 0.84 -0.0 3,722,822 - -0.2 3,722,901 + +0.3 3,722,991 + -1.1 3,723,576 + Shew_3131 0.42 -2.4 3,723,992 + Shew_3131 0.71 -2.9 3,724,239 + Shew_3131 0.88 -2.2 3,724,310 + -0.5
Or see this region's nucleotide sequence