Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2482 and dinG are separated by 162 nucleotides dinG and Shew_2484 are separated by 38 nucleotides Shew_2484 and Shew_2485 overlap by 4 nucleotides
Shew_2482: Shew_2482 - DNA polymerase II (RefSeq), at 2,919,819 to 2,922,206
_2482
Shew_2483: dinG - ATP-dependent DNA helicase DinG (RefSeq), at 2,922,369 to 2,924,441
dinG
Shew_2484: Shew_2484 - hypothetical protein (RefSeq), at 2,924,480 to 2,925,253
_2484
Shew_2485: Shew_2485 - primosomal replication protein N'', putative (RefSeq), at 2,925,250 to 2,925,912
_2485
Position (kb)
2922
2923
2924
2925 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2921.399 kb on - strand, within Shew_2482 at 2921.496 kb on - strand, within Shew_2482 at 2921.822 kb on - strand, within Shew_2482 at 2921.822 kb on - strand, within Shew_2482 at 2922.188 kb on - strand at 2922.363 kb on + strand at 2922.371 kb on - strand at 2922.668 kb on - strand, within dinG at 2922.951 kb on - strand, within dinG at 2923.422 kb on + strand, within dinG at 2923.744 kb on - strand, within dinG at 2923.754 kb on - strand, within dinG at 2923.754 kb on - strand, within dinG at 2923.968 kb on - strand, within dinG at 2925.232 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 2,921,399 - Shew_2482 0.66 +0.7 2,921,496 - Shew_2482 0.70 -1.2 2,921,822 - Shew_2482 0.84 -1.4 2,921,822 - Shew_2482 0.84 -0.5 2,922,188 - -0.3 2,922,363 + -0.4 2,922,371 - +0.2 2,922,668 - dinG Shew_2483 0.14 +0.2 2,922,951 - dinG Shew_2483 0.28 -0.3 2,923,422 + dinG Shew_2483 0.51 +0.3 2,923,744 - dinG Shew_2483 0.66 -0.8 2,923,754 - dinG Shew_2483 0.67 -0.4 2,923,754 - dinG Shew_2483 0.67 -0.4 2,923,968 - dinG Shew_2483 0.77 -0.8 2,925,232 + -3.6
Or see this region's nucleotide sequence