Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1823 and Shew_1824 are separated by 224 nucleotides Shew_1824 and uvrC are separated by 31 nucleotides uvrC and Shew_1826 are separated by 47 nucleotides Shew_1826 and Shew_R0061 are separated by 140 nucleotides Shew_R0061 and Shew_R0062 are separated by 12 nucleotides Shew_R0062 and Shew_R0063 are separated by 39 nucleotides
Shew_1823: Shew_1823 - putative AcnD-accessory protein PrpF (RefSeq), at 2,097,828 to 2,098,997
_1823
Shew_1824: Shew_1824 - response regulator (RefSeq), at 2,099,222 to 2,099,911
_1824
Shew_1825: uvrC - excinuclease ABC subunit C (RefSeq), at 2,099,943 to 2,101,772
uvrC
Shew_1826: Shew_1826 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (RefSeq), at 2,101,820 to 2,102,377
_1826
Shew_R0061: Shew_R0061 - tRNA-Gly (RefSeq), at 2,102,518 to 2,102,593
_R0061
Shew_R0062: Shew_R0062 - tRNA-Cys (RefSeq), at 2,102,606 to 2,102,679
_R0062
Shew_R0063: Shew_R0063 - tRNA-Leu (RefSeq), at 2,102,719 to 2,102,805
_R0063
Position (kb)
2099
2100
2101
2102 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2099.961 kb on - strand at 2100.461 kb on + strand, within uvrC at 2100.544 kb on + strand, within uvrC at 2100.978 kb on + strand, within uvrC at 2101.073 kb on + strand, within uvrC at 2101.496 kb on - strand, within uvrC
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 2,099,961 - -2.5 2,100,461 + uvrC Shew_1825 0.28 -1.9 2,100,544 + uvrC Shew_1825 0.33 -1.4 2,100,978 + uvrC Shew_1825 0.57 -2.0 2,101,073 + uvrC Shew_1825 0.62 -2.8 2,101,496 - uvrC Shew_1825 0.85 -2.5
Or see this region's nucleotide sequence