Experiment: LB 10C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0540 and Shew_0541 are separated by 49 nucleotides Shew_0541 and Shew_0542 are separated by 12 nucleotides Shew_0542 and Shew_0543 are separated by 4 nucleotides Shew_0543 and tolC are separated by 67 nucleotides
Shew_0540: Shew_0540 - enoyl-CoA hydratase/isomerase (RefSeq), at 611,424 to 612,161
_0540
Shew_0541: Shew_0541 - hypothetical protein (RefSeq), at 612,211 to 612,678
_0541
Shew_0542: Shew_0542 - hypothetical protein (RefSeq), at 612,691 to 613,662
_0542
Shew_0543: Shew_0543 - hypothetical protein (RefSeq), at 613,667 to 614,425
_0543
Shew_0544: tolC - outer membrane channel protein (RefSeq), at 614,493 to 615,839
tolC
Position (kb) 612
613
614 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 611.817 kb on - strand, within Shew_0540 at 611.892 kb on - strand, within Shew_0540 at 612.080 kb on + strand, within Shew_0540 at 612.560 kb on + strand, within Shew_0541 at 612.689 kb on - strand at 612.704 kb on - strand at 612.785 kb on + strand at 612.785 kb on + strand at 612.819 kb on + strand, within Shew_0542 at 613.228 kb on + strand, within Shew_0542 at 613.295 kb on - strand, within Shew_0542 at 613.425 kb on - strand, within Shew_0542 at 613.490 kb on - strand, within Shew_0542 at 613.564 kb on - strand, within Shew_0542 at 613.581 kb on - strand at 613.702 kb on - strand at 614.025 kb on + strand, within Shew_0543 at 614.249 kb on - strand, within Shew_0543 at 614.414 kb on - strand at 614.637 kb on - strand, within tolC
Per-strain Table
Position Strand Gene LocusTag Fraction LB 10C remove 611,817 - Shew_0540 0.53 -0.2 611,892 - Shew_0540 0.63 -1.6 612,080 + Shew_0540 0.89 -0.0 612,560 + Shew_0541 0.75 +0.1 612,689 - -0.7 612,704 - -0.8 612,785 + -0.5 612,785 + -0.9 612,819 + Shew_0542 0.13 -0.2 613,228 + Shew_0542 0.55 +0.1 613,295 - Shew_0542 0.62 -1.6 613,425 - Shew_0542 0.76 -0.6 613,490 - Shew_0542 0.82 -1.8 613,564 - Shew_0542 0.90 -2.0 613,581 - -1.0 613,702 - -2.5 614,025 + Shew_0543 0.47 -1.9 614,249 - Shew_0543 0.77 -1.4 614,414 - -1.7 614,637 - tolC Shew_0544 0.11 -4.3
Or see this region's nucleotide sequence