Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1667 and Dshi_1668 overlap by 4 nucleotides Dshi_1668 and Dshi_1669 are separated by 5 nucleotides Dshi_1669 and Dshi_1670 overlap by 4 nucleotides Dshi_1670 and Dshi_1671 are separated by 16 nucleotides
Dshi_1667: Dshi_1667 - Nitrite reductase (NAD(P)H) (EC 1.7.1.4) (from data) , at 1,733,528 to 1,735,960
_1667
Dshi_1668: Dshi_1668 - nitrite reductase (NAD(P)H), small subunit (RefSeq), at 1,735,957 to 1,736,298
_1668
Dshi_1669: Dshi_1669 - Nitrate reductase (EC 1.7.99.4) (from data) , at 1,736,304 to 1,738,925
_1669
Dshi_1670: Dshi_1670 - glycosyl transferase family 3 (RefSeq), at 1,738,922 to 1,739,893
_1670
Dshi_1671: Dshi_1671 - uroporphyrin-III C-methyltransferase (RefSeq), at 1,739,910 to 1,741,304
_1671
Position (kb)
1736
1737
1738
1739 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1735.474 kb on + strand, within Dshi_1667 at 1735.484 kb on - strand, within Dshi_1667 at 1735.484 kb on - strand, within Dshi_1667 at 1735.516 kb on + strand, within Dshi_1667 at 1735.516 kb on + strand, within Dshi_1667 at 1735.524 kb on - strand, within Dshi_1667 at 1735.526 kb on - strand, within Dshi_1667 at 1735.526 kb on - strand, within Dshi_1667 at 1735.555 kb on - strand, within Dshi_1667 at 1735.760 kb on - strand at 1735.835 kb on - strand at 1735.936 kb on + strand at 1736.028 kb on - strand, within Dshi_1668 at 1736.112 kb on - strand, within Dshi_1668 at 1736.148 kb on - strand, within Dshi_1668 at 1736.235 kb on + strand, within Dshi_1668 at 1736.254 kb on + strand, within Dshi_1668 at 1736.295 kb on - strand at 1736.310 kb on + strand at 1736.428 kb on - strand at 1736.433 kb on - strand at 1736.448 kb on - strand at 1736.457 kb on + strand at 1736.568 kb on - strand, within Dshi_1669 at 1736.845 kb on - strand, within Dshi_1669 at 1736.864 kb on - strand, within Dshi_1669 at 1736.896 kb on + strand, within Dshi_1669 at 1736.896 kb on + strand, within Dshi_1669 at 1737.008 kb on + strand, within Dshi_1669 at 1737.049 kb on - strand, within Dshi_1669 at 1737.066 kb on + strand, within Dshi_1669 at 1737.066 kb on + strand, within Dshi_1669 at 1737.074 kb on - strand, within Dshi_1669 at 1737.096 kb on + strand, within Dshi_1669 at 1737.274 kb on - strand, within Dshi_1669 at 1737.384 kb on - strand, within Dshi_1669 at 1737.407 kb on - strand, within Dshi_1669 at 1737.451 kb on - strand, within Dshi_1669 at 1737.501 kb on - strand, within Dshi_1669 at 1737.672 kb on - strand, within Dshi_1669 at 1737.684 kb on - strand, within Dshi_1669 at 1737.733 kb on - strand, within Dshi_1669 at 1737.791 kb on + strand, within Dshi_1669 at 1737.804 kb on + strand, within Dshi_1669 at 1737.830 kb on - strand, within Dshi_1669 at 1737.923 kb on + strand, within Dshi_1669 at 1737.992 kb on - strand, within Dshi_1669 at 1738.025 kb on + strand, within Dshi_1669 at 1738.177 kb on - strand, within Dshi_1669 at 1738.228 kb on + strand, within Dshi_1669 at 1738.282 kb on - strand, within Dshi_1669 at 1738.312 kb on + strand, within Dshi_1669 at 1738.398 kb on - strand, within Dshi_1669 at 1738.530 kb on - strand, within Dshi_1669 at 1738.951 kb on + strand at 1738.994 kb on - strand at 1739.018 kb on - strand at 1739.054 kb on + strand, within Dshi_1670 at 1739.056 kb on + strand, within Dshi_1670 at 1739.175 kb on + strand, within Dshi_1670 at 1739.249 kb on - strand, within Dshi_1670 at 1739.280 kb on - strand, within Dshi_1670 at 1739.303 kb on + strand, within Dshi_1670 at 1739.366 kb on - strand, within Dshi_1670 at 1739.607 kb on - strand, within Dshi_1670 at 1739.685 kb on - strand, within Dshi_1670 at 1739.833 kb on - strand at 1739.857 kb on + strand at 1739.910 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 1,735,474 + Dshi_1667 0.80 +0.2 1,735,484 - Dshi_1667 0.80 -0.2 1,735,484 - Dshi_1667 0.80 -0.8 1,735,516 + Dshi_1667 0.82 +1.4 1,735,516 + Dshi_1667 0.82 -0.4 1,735,524 - Dshi_1667 0.82 -0.1 1,735,526 - Dshi_1667 0.82 -0.2 1,735,526 - Dshi_1667 0.82 +1.4 1,735,555 - Dshi_1667 0.83 -0.5 1,735,760 - +0.5 1,735,835 - -0.7 1,735,936 + -1.0 1,736,028 - Dshi_1668 0.21 +0.0 1,736,112 - Dshi_1668 0.45 -1.4 1,736,148 - Dshi_1668 0.56 -0.8 1,736,235 + Dshi_1668 0.81 -2.3 1,736,254 + Dshi_1668 0.87 +0.1 1,736,295 - -0.4 1,736,310 + -0.8 1,736,428 - -0.5 1,736,433 - -0.8 1,736,448 - +0.4 1,736,457 + -1.9 1,736,568 - Dshi_1669 0.10 -0.4 1,736,845 - Dshi_1669 0.21 +0.2 1,736,864 - Dshi_1669 0.21 -0.8 1,736,896 + Dshi_1669 0.23 +0.4 1,736,896 + Dshi_1669 0.23 +0.1 1,737,008 + Dshi_1669 0.27 -0.6 1,737,049 - Dshi_1669 0.28 +0.3 1,737,066 + Dshi_1669 0.29 -0.0 1,737,066 + Dshi_1669 0.29 -0.8 1,737,074 - Dshi_1669 0.29 -0.7 1,737,096 + Dshi_1669 0.30 -0.5 1,737,274 - Dshi_1669 0.37 -0.4 1,737,384 - Dshi_1669 0.41 -0.5 1,737,407 - Dshi_1669 0.42 +0.3 1,737,451 - Dshi_1669 0.44 -0.8 1,737,501 - Dshi_1669 0.46 +0.4 1,737,672 - Dshi_1669 0.52 -0.3 1,737,684 - Dshi_1669 0.53 +0.6 1,737,733 - Dshi_1669 0.55 +0.0 1,737,791 + Dshi_1669 0.57 -0.4 1,737,804 + Dshi_1669 0.57 -0.8 1,737,830 - Dshi_1669 0.58 -0.7 1,737,923 + Dshi_1669 0.62 -1.3 1,737,992 - Dshi_1669 0.64 +0.0 1,738,025 + Dshi_1669 0.66 +0.2 1,738,177 - Dshi_1669 0.71 +0.1 1,738,228 + Dshi_1669 0.73 -0.7 1,738,282 - Dshi_1669 0.75 -0.9 1,738,312 + Dshi_1669 0.77 -0.1 1,738,398 - Dshi_1669 0.80 -0.1 1,738,530 - Dshi_1669 0.85 -2.0 1,738,951 + -0.1 1,738,994 - -0.4 1,739,018 - +0.5 1,739,054 + Dshi_1670 0.14 +1.2 1,739,056 + Dshi_1670 0.14 +0.3 1,739,175 + Dshi_1670 0.26 -0.5 1,739,249 - Dshi_1670 0.34 -0.5 1,739,280 - Dshi_1670 0.37 -0.4 1,739,303 + Dshi_1670 0.39 -0.3 1,739,366 - Dshi_1670 0.46 -0.7 1,739,607 - Dshi_1670 0.70 -0.9 1,739,685 - Dshi_1670 0.78 -2.9 1,739,833 - -2.6 1,739,857 + -1.2 1,739,910 + -1.2
Or see this region's nucleotide sequence