Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_R0007 and Dshi_R0008 are separated by 175 nucleotides Dshi_R0008 and Dshi_6009 are separated by 452 nucleotides Dshi_6009 and Dshi_6010 are separated by 196 nucleotides Dshi_6010 and Dshi_R0011 are separated by 66 nucleotides Dshi_R0011 and Dshi_0391 are separated by 310 nucleotides
Dshi_R0007: Dshi_R0007 - tRNA-Ile (RefSeq), at 375,220 to 375,296
_R0007
Dshi_R0008: Dshi_R0008 - tRNA-Ala (RefSeq), at 375,472 to 375,547
_R0008
Dshi_6009: Dshi_6009 - 23SrRNA (RefSeq), at 376,000 to 378,915
_6009
Dshi_6010: Dshi_6010 - 5SrRNA (RefSeq), at 379,112 to 379,229
_6010
Dshi_R0011: Dshi_R0011 - tRNA-Met (RefSeq), at 379,296 to 379,372
_R0011
Dshi_0391: Dshi_0391 - hypothetical protein (RefSeq), at 379,683 to 382,190
_0391
Position (kb) 375
376
377
378
379 Strain fitness (log2 ratio) -1
0
1 at 379.508 kb on + strand at 379.535 kb on + strand at 379.543 kb on - strand at 379.600 kb on - strand at 379.639 kb on + strand at 379.652 kb on + strand at 379.711 kb on - strand at 379.771 kb on + strand at 379.786 kb on + strand at 379.845 kb on + strand at 379.888 kb on + strand at 379.903 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 379,508 + -0.3 379,535 + +0.0 379,543 - +1.2 379,600 - -0.3 379,639 + +0.0 379,652 + -0.5 379,711 - -0.5 379,771 + +0.2 379,786 + -0.3 379,845 + +0.4 379,888 + -0.6 379,903 - -0.0
Or see this region's nucleotide sequence