Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0228 and Dshi_0229 are separated by 4 nucleotides Dshi_0229 and Dshi_0230 are separated by 346 nucleotides Dshi_0230 and Dshi_0231 are separated by 12 nucleotides
Dshi_0228: Dshi_0228 - pyruvate kinase (RefSeq), at 225,543 to 226,991
_0228
Dshi_0229: Dshi_0229 - hypothetical protein (RefSeq), at 226,996 to 227,220
_0229
Dshi_0230: Dshi_0230 - ribosomal protein L35 (RefSeq), at 227,567 to 227,767
_0230
Dshi_0231: Dshi_0231 - ribosomal protein L20 (RefSeq), at 227,780 to 228,145
_0231
Position (kb) 226
227
228 Strain fitness (log2 ratio) -1
0
1 at 226.496 kb on + strand, within Dshi_0228 at 226.889 kb on - strand at 227.010 kb on + strand at 227.016 kb on + strand at 227.209 kb on + strand at 227.217 kb on - strand at 227.242 kb on + strand at 227.274 kb on + strand at 227.326 kb on - strand at 227.467 kb on - strand at 228.121 kb on + strand at 228.124 kb on - strand at 228.129 kb on - strand at 228.129 kb on - strand at 228.177 kb on + strand at 228.177 kb on + strand at 228.185 kb on - strand at 228.209 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 226,496 + Dshi_0228 0.66 -0.2 226,889 - +0.3 227,010 + -0.2 227,016 + -0.0 227,209 + -1.5 227,217 - -0.2 227,242 + -0.6 227,274 + +0.5 227,326 - +0.3 227,467 - -0.1 228,121 + +0.8 228,124 - -0.8 228,129 - +0.8 228,129 - +0.3 228,177 + -0.1 228,177 + -0.2 228,185 - -0.3 228,209 - -0.3
Or see this region's nucleotide sequence