Experiment: Min media with glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00010 and SMc00450 overlap by 4 nucleotides SMc00450 and SMc00130 overlap by 1 nucleotides SMc00130 and SMc00012 are separated by 11 nucleotides SMc00012 and SMc00013 are separated by 61 nucleotides
SMc00010: SMc00010 - cytochrome C oxidase polypeptide I transmembrane protein, at 992,942 to 994,630
SMc00010
SMc00450: SMc00450 - protoheme IX farnesyltransferase, at 994,627 to 995,679
SMc00450
SMc00130: SMc00130 - hypothetical protein, at 995,679 to 995,819
SMc00130
SMc00012: SMc00012 - cytochrome C oxidase assembly protein, at 995,831 to 996,427
SMc00012
SMc00013: SMc00013 - cytochrome C oxidase subunit III transmembrane protein, at 996,489 to 997,367
SMc00013
Position (kb) 994
995
996 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 994.200 kb on - strand, within SMc00010 at 994.220 kb on + strand, within SMc00010 at 994.298 kb on + strand, within SMc00010 at 994.722 kb on + strand at 995.143 kb on + strand, within SMc00450 at 995.155 kb on + strand, within SMc00450 at 995.420 kb on - strand, within SMc00450 at 995.927 kb on + strand, within SMc00012 at 995.972 kb on + strand, within SMc00012 at 996.321 kb on + strand, within SMc00012 at 996.472 kb on + strand at 996.519 kb on + strand at 996.519 kb on + strand at 996.519 kb on + strand at 996.519 kb on + strand at 996.519 kb on + strand at 996.520 kb on - strand at 996.607 kb on - strand, within SMc00013
Per-strain Table
Position Strand Gene LocusTag Fraction Min media with glucose remove 994,200 - SMc00010 0.74 -1.6 994,220 + SMc00010 0.76 -0.3 994,298 + SMc00010 0.80 -1.2 994,722 + -1.3 995,143 + SMc00450 0.49 -2.9 995,155 + SMc00450 0.50 -0.4 995,420 - SMc00450 0.75 -2.0 995,927 + SMc00012 0.16 -2.4 995,972 + SMc00012 0.24 -0.4 996,321 + SMc00012 0.82 -1.3 996,472 + -0.9 996,519 + -0.2 996,519 + -2.0 996,519 + -1.0 996,519 + +0.6 996,519 + +1.3 996,520 - +1.1 996,607 - SMc00013 0.13 +0.3
Or see this region's nucleotide sequence